FastQCFastQC Report
Fri 10 Feb 2017
ERR1632181.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632181.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525589
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20740.1359474930666123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20120.13188348893443777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10600.025.1320761
GTATCAA15850.016.8075712
CGAATTA1701.4861143E-915.23529315
ATATAGG750.004105224314.81
TCTTATA11400.014.44298437
TCTGTCG908.276204E-414.3888888
CTCTTAT18550.014.26145637
CTTATAC8100.014.16049437
TAGCACT2101.364242E-1014.0952384
CCCTTAC1051.6561919E-414.0952381
TGCGTTA1601.7842831E-713.87500137
ACGCGTA1203.3017517E-513.87499917
TTATAGT1102.4571855E-413.4545454
ATGCGTT1805.1610186E-813.36111136
TATGCCG1409.562937E-613.21428533
CTATAAG850.00940657613.0588231
GGACCTA850.00940657613.0588236
CTAGATA1153.5783622E-412.8695654
TTTAGGC2305.7480065E-1012.8695653
TTTAGAC1153.5783622E-412.8695653
Produced by FastQC (version 0.11.5)