##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632181.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1525589
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.233985693394487	31.0	31.0	34.0	30.0	34.0
2	31.39885119779967	31.0	31.0	34.0	30.0	34.0
3	31.417750127983357	31.0	31.0	34.0	28.0	34.0
4	35.30179950170066	37.0	35.0	37.0	33.0	37.0
5	34.404955725296915	35.0	35.0	37.0	30.0	37.0
6	34.348710563592164	35.0	35.0	37.0	30.0	37.0
7	34.357203676743865	35.0	35.0	37.0	30.0	37.0
8	34.288479400415184	35.0	35.0	37.0	30.0	37.0
9	35.86933636778975	37.0	35.0	39.0	31.0	39.0
10	35.51166664153976	37.0	35.0	39.0	30.0	39.0
11	35.74653461712165	37.0	35.0	39.0	30.0	39.0
12	35.6517613852748	37.0	35.0	39.0	30.0	39.0
13	35.787128774525776	37.0	35.0	39.0	30.0	39.0
14	36.72466634198332	38.0	36.0	40.0	31.0	41.0
15	36.671701224903956	38.0	36.0	40.0	31.0	41.0
16	36.54451362719579	38.0	36.0	40.0	31.0	41.0
17	36.55837778064734	38.0	36.0	40.0	31.0	41.0
18	36.55201040385058	38.0	36.0	40.0	31.0	41.0
19	36.62639347819105	38.0	36.0	40.0	31.0	41.0
20	36.583398280926254	38.0	35.0	40.0	31.0	41.0
21	36.5361083489721	38.0	35.0	40.0	31.0	41.0
22	36.513272578656505	38.0	35.0	40.0	30.0	41.0
23	36.449212730296296	38.0	35.0	40.0	30.0	41.0
24	36.34366464362289	38.0	35.0	40.0	30.0	41.0
25	36.23366581694021	38.0	35.0	40.0	30.0	41.0
26	36.05742634484124	38.0	35.0	40.0	30.0	41.0
27	35.94763334030332	38.0	34.0	40.0	29.0	41.0
28	35.82813654267303	38.0	34.0	40.0	29.0	41.0
29	35.635651541797955	38.0	34.0	40.0	28.0	41.0
30	35.542109965396975	38.0	34.0	40.0	27.0	41.0
31	35.40656559532089	38.0	34.0	40.0	27.0	41.0
32	35.28237225097978	38.0	34.0	40.0	27.0	41.0
33	35.09938325459871	38.0	33.0	40.0	27.0	40.0
34	35.07344507596738	38.0	33.0	40.0	27.0	40.0
35	35.01839092966716	38.0	33.0	40.0	26.0	40.0
36	34.98015127272155	38.0	33.0	40.0	26.0	40.0
37	34.94503499959688	37.0	33.0	40.0	26.0	40.0
38	34.8450854063578	37.0	33.0	40.0	26.0	40.0
39	34.643913268907944	37.0	33.0	39.0	25.0	40.0
40	34.6168161936144	37.0	33.0	39.0	26.0	40.0
41	34.59186845211915	37.0	33.0	39.0	26.0	40.0
42	34.44994294007102	37.0	33.0	39.0	25.0	40.0
43	33.49689726394199	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	3.0
12	5.0
13	10.0
14	21.0
15	41.0
16	79.0
17	138.0
18	285.0
19	519.0
20	963.0
21	1566.0
22	2520.0
23	4018.0
24	6138.0
25	9159.0
26	13324.0
27	18810.0
28	26209.0
29	36047.0
30	47843.0
31	63234.0
32	83534.0
33	109539.0
34	142441.0
35	183784.0
36	228343.0
37	255007.0
38	220691.0
39	71317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.23962023847839	18.96736276939595	12.552070052943487	26.24094693918218
2	20.431977419868655	20.93611057761953	32.629233692691805	26.00267830982001
3	21.316029415524103	22.021330777817617	29.15634551638744	27.506294290270837
4	16.64714415219302	15.838866169066504	32.63972144529097	34.874268233449506
5	16.425852572350745	34.73255247645336	32.81709556112426	16.02449939007164
6	35.89498875516276	33.407031644827015	15.011382488992774	15.68659711101745
7	30.071533027571647	29.203605951537405	20.46711139107584	20.25774962981511
8	27.679604401971957	32.18678162991474	19.589024304711163	20.544589663402135
9	26.92730479834346	13.973553820852144	19.0348121282993	40.0643292525051
10	17.857430802136093	26.212498910256954	32.367564265342764	23.562506022264188
11	36.99056561105252	20.855617076420977	20.56412310261807	21.589694209908437
12	22.514058504616905	24.535966108827477	27.800803492946002	25.14917189360962
13	31.642991657648288	18.9111877445367	23.459922692153654	25.985897905661353
14	23.84167688676308	20.05291071186276	23.620778597643273	32.48463380373089
15	26.67428776688872	26.57491631101168	20.99045024577393	25.76034567632567
16	26.391970576610085	25.07457775324809	22.87726248681657	25.65618918332526
17	25.08591763574593	25.424475399337567	24.1311388584999	25.358468106416606
18	24.963604221058226	24.29966393307765	25.146156664737358	25.590575181126763
19	25.974885765432237	24.474743853029878	25.188894256578937	24.36147612495895
20	26.101656474974583	24.046646901622914	24.848828878551167	25.002867744851333
21	26.12571275749891	24.410703013721257	24.046188062446703	25.417396166333134
22	26.357492089940344	24.118684652288394	24.051825229468747	25.47199802830251
23	25.831203554823745	24.280392687676695	24.2128777803196	25.675525977179962
24	26.084810522362183	24.231427992729365	24.04481154491806	25.63894993999039
25	26.167794864803035	23.76111783711078	24.332044869227555	25.73904242885862
26	25.853031189920745	24.20239002772044	24.275279908284602	25.669298874074208
27	25.792726612475576	24.121109945076952	24.145035130693785	25.94112831175369
28	25.47035931695889	24.29868070627148	24.675191024581324	25.555768952188302
29	25.455938657135047	24.39005525079166	24.74880193813668	25.405204153936612
30	25.290887650605764	24.945906138547144	24.412735015787344	25.350471195059743
31	25.834218783696	24.360296252791542	24.44216627151874	25.363318691993715
32	25.142682596688886	24.31480562589269	24.726777657678443	25.81573411973998
33	25.15946300084754	24.20016138029312	24.71635545353303	25.92402016532631
34	25.74487624124191	23.80621517328717	24.59522191101273	25.853686674458192
35	25.375576252843985	24.032619532521537	25.09502887081645	25.49677534381803
36	25.139732916270376	24.15021345853962	24.964390802503164	25.745662822686842
37	25.572090517170743	23.009867008742198	25.33618163214339	26.081860841943673
38	24.711504867955917	23.61527252752871	26.011002963445595	25.66221964106978
39	24.667980694669403	23.295199427893095	26.357492089940344	25.679327787497158
40	24.393136028117667	22.947202686962214	27.064956551207437	25.594704733712685
41	23.928528587974874	23.096390967685267	27.25144190211125	25.72363854222861
42	24.197998281319542	22.802275055732572	27.09995942550713	25.899767237440752
43	23.478538453017162	22.733186985485606	27.50635983872459	26.281914722772648
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	48.0
1	47.0
2	46.0
3	139.0
4	232.0
5	232.0
6	319.0
7	406.0
8	424.0
9	442.0
10	644.5
11	847.0
12	847.0
13	1465.0
14	2083.0
15	3418.0
16	4753.0
17	4703.0
18	4653.0
19	4653.0
20	6112.0
21	7571.0
22	8188.0
23	8805.0
24	10600.0
25	12395.0
26	12395.0
27	14356.5
28	16318.0
29	20674.5
30	25031.0
31	29306.5
32	33582.0
33	33582.0
34	39006.0
35	44430.0
36	50705.5
37	56981.0
38	63946.0
39	70911.0
40	70911.0
41	77488.0
42	84065.0
43	86275.0
44	88485.0
45	94576.5
46	100668.0
47	100668.0
48	104645.0
49	108622.0
50	114112.0
51	119602.0
52	123670.5
53	127739.0
54	127739.0
55	119602.5
56	111466.0
57	107180.0
58	102894.0
59	96360.0
60	89826.0
61	89826.0
62	84080.0
63	78334.0
64	70918.0
65	63502.0
66	56326.5
67	49151.0
68	49151.0
69	43077.5
70	37004.0
71	32142.5
72	27281.0
73	22863.0
74	18445.0
75	18445.0
76	15021.5
77	11598.0
78	9637.5
79	7677.0
80	6071.5
81	4466.0
82	4466.0
83	3529.0
84	2592.0
85	2022.5
86	1453.0
87	1081.0
88	709.0
89	709.0
90	530.5
91	352.0
92	224.0
93	96.0
94	59.5
95	23.0
96	23.0
97	14.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1525589.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.9670847057215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.99899353055433	56.520709820120395
2	7.799509004728103	10.134227243464164
3	2.0570641548102397	4.009243835719807
4	0.9014325128173107	2.3425376966677445
5	0.4953648040942108	1.6091203593910863
6	0.314417931493413	1.2256089832998183
7	0.21962650840658965	0.9987945782690936
8	0.154302396159468	0.8019661473270363
9	0.12328724965874549	0.7208651872543637
>10	0.7860712477683914	9.957339290258655
>50	0.09124951782406246	4.150554730264732
>100	0.05695826575909915	6.563008434337646
>500	0.0014188389979132355	0.6065301435167629
>1k	3.0403692812426477E-4	0.35949355010871964
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2074	0.1359474930666123	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2012	0.13188348893443777	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	6.55484537447504E-5	0.0
13	0.0	0.0	0.0	6.55484537447504E-5	0.0
14	0.0	0.0	0.0	1.310969074895008E-4	0.0
15	0.0	0.0	0.0	1.9664536123425116E-4	0.0
16	0.0	0.0	0.0	3.932907224685023E-4	0.0
17	0.0	0.0	0.0	4.588391762132527E-4	0.0
18	0.0	0.0	0.0	4.588391762132527E-4	0.0
19	0.0	0.0	0.0	5.899360837027535E-4	0.0
20	0.0	0.0	0.0	7.210329911922543E-4	0.0
21	0.0	0.0	0.0	9.176783524265054E-4	0.0
22	0.0	6.55484537447504E-5	0.0	0.0013109690748950077	0.0
23	0.0	6.55484537447504E-5	0.0	0.0020975505198320126	0.0
24	0.0	6.55484537447504E-5	0.0	0.003211874233492769	0.0
25	0.0	6.55484537447504E-5	0.0	0.003998455678429774	0.0
26	0.0	6.55484537447504E-5	0.0	0.006620393828219789	0.0
27	0.0	6.55484537447504E-5	0.0	0.019730084577169866	0.0
28	0.0	6.55484537447504E-5	0.0	0.05597837949801683	0.0
29	0.0	6.55484537447504E-5	0.0	0.10638514042772988	0.0
30	0.0	6.55484537447504E-5	0.0	0.17232688489494877	0.0
31	0.0	6.55484537447504E-5	0.0	0.35704242754765536	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1060	0.0	25.132076	1
GTATCAA	1585	0.0	16.807571	2
CGAATTA	170	1.4861143E-9	15.235293	15
ATATAGG	75	0.0041052243	14.8	1
TCTTATA	1140	0.0	14.442984	37
TCTGTCG	90	8.276204E-4	14.388888	8
CTCTTAT	1855	0.0	14.261456	37
CTTATAC	810	0.0	14.160494	37
TAGCACT	210	1.364242E-10	14.095238	4
CCCTTAC	105	1.6561919E-4	14.095238	1
TGCGTTA	160	1.7842831E-7	13.875001	37
ACGCGTA	120	3.3017517E-5	13.874999	17
TTATAGT	110	2.4571855E-4	13.454545	4
ATGCGTT	180	5.1610186E-8	13.361111	36
TATGCCG	140	9.562937E-6	13.214285	33
CTATAAG	85	0.009406576	13.058823	1
GGACCTA	85	0.009406576	13.058823	6
CTAGATA	115	3.5783622E-4	12.869565	4
TTTAGGC	230	5.7480065E-10	12.869565	3
TTTAGAC	115	3.5783622E-4	12.869565	3
>>END_MODULE