FastQCFastQC Report
Fri 10 Feb 2017
ERR1632180.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632180.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741553
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4650.019.0967751
ATACAGT500.00703258618.56
CTTATAC5500.017.15454737
GTATCAA5400.016.4444452
TATGTAC803.3805406E-416.18753
CATTAGG700.002591331315.8571422
TACATCG700.002591331315.8571425
GTGTACA700.002591331315.8571421
GGGTAAC2450.015.10204125
GTCATAC750.004102580214.81
TTAATTG750.004102580214.83
GTGATTA750.004102580214.814
CGGGTAA2500.014.79999924
GCGGGTA2400.014.64583323
GGTAACC2550.014.50980426
TAACCCG2301.8189894E-1214.4782628
ATACTCT908.269137E-414.3888876
TCGTCAC1551.2079181E-714.32258119
ACCCGTT2353.6379788E-1214.17021430
GTAACCC2500.014.05999927
Produced by FastQC (version 0.11.5)