##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632180.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	741553
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79476854655028	34.0	31.0	34.0	31.0	34.0
2	32.95480565785588	34.0	31.0	34.0	31.0	34.0
3	32.949942890123836	34.0	33.0	34.0	31.0	34.0
4	36.39074348023674	37.0	37.0	37.0	35.0	37.0
5	36.30768940318494	37.0	37.0	37.0	35.0	37.0
6	36.336627321310814	37.0	37.0	37.0	35.0	37.0
7	36.326383953675595	37.0	37.0	37.0	35.0	37.0
8	36.314752957644295	37.0	37.0	37.0	35.0	37.0
9	38.10297443338507	39.0	39.0	39.0	37.0	39.0
10	38.03675664450147	39.0	38.0	39.0	35.0	39.0
11	38.14054423621778	39.0	39.0	39.0	37.0	39.0
12	38.10964961371608	39.0	38.0	39.0	37.0	39.0
13	38.136808832274966	39.0	39.0	39.0	37.0	39.0
14	39.53941795124556	41.0	39.0	41.0	37.0	41.0
15	39.56910564720256	41.0	39.0	41.0	37.0	41.0
16	39.44061719121897	41.0	39.0	41.0	37.0	41.0
17	39.48947276863555	41.0	39.0	41.0	37.0	41.0
18	39.50168228029554	41.0	39.0	41.0	37.0	41.0
19	39.545333914096496	41.0	39.0	41.0	37.0	41.0
20	39.52817263230005	41.0	39.0	41.0	37.0	41.0
21	39.50070864793211	41.0	39.0	41.0	37.0	41.0
22	39.4838467378596	41.0	39.0	41.0	37.0	41.0
23	39.4416676892953	41.0	39.0	41.0	37.0	41.0
24	39.38972264962855	41.0	39.0	41.0	37.0	41.0
25	39.326454076782106	40.0	39.0	41.0	36.0	41.0
26	39.252610400065805	40.0	39.0	41.0	36.0	41.0
27	39.18523962548867	40.0	39.0	41.0	36.0	41.0
28	39.11038995189825	40.0	39.0	41.0	36.0	41.0
29	38.97518855698784	40.0	39.0	41.0	35.0	41.0
30	38.93515365725713	40.0	39.0	41.0	35.0	41.0
31	38.868827986671214	40.0	38.0	41.0	35.0	41.0
32	38.80641033075182	40.0	38.0	41.0	35.0	41.0
33	38.65164863468963	40.0	38.0	41.0	35.0	41.0
34	38.64657684615934	40.0	38.0	41.0	35.0	41.0
35	38.617073897617566	40.0	38.0	41.0	35.0	41.0
36	38.58257467773713	40.0	38.0	41.0	35.0	41.0
37	38.543557911572066	40.0	38.0	41.0	35.0	41.0
38	38.47290618472314	40.0	38.0	41.0	34.0	41.0
39	38.27399390198678	40.0	38.0	41.0	34.0	41.0
40	38.27058079462965	40.0	38.0	41.0	34.0	41.0
41	38.27910075207032	40.0	37.0	41.0	34.0	41.0
42	38.127278832396335	40.0	37.0	41.0	34.0	41.0
43	37.240682729353125	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	1.0
14	1.0
15	1.0
16	0.0
17	2.0
18	12.0
19	15.0
20	34.0
21	72.0
22	144.0
23	253.0
24	459.0
25	636.0
26	1008.0
27	1502.0
28	2182.0
29	3051.0
30	4382.0
31	5919.0
32	8314.0
33	11742.0
34	17704.0
35	26958.0
36	43990.0
37	79608.0
38	185224.0
39	348337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.8520240630137	16.893060914054693	11.549005937539192	27.70590908539241
2	21.56339465958603	18.837763450488367	31.172552737295916	28.426289152629685
3	21.890411069741475	19.757724667016383	27.463310107301837	30.8885541559403
4	16.43860924303455	14.456957223556511	30.993334259317944	38.111099274090996
5	18.238615446232433	32.68208745699903	31.477318546348005	17.601978550420537
6	38.57054047384341	30.98632194866719	14.191837940106774	16.251299637382626
7	32.35749838514577	26.92727289890271	19.213326626687504	21.501902089264018
8	29.152063304982924	29.925709962740356	18.530974859517794	22.39125187275893
9	27.545839609576117	13.101693338170028	18.155546535446557	41.196920516807296
10	19.52267740808816	23.844013846616495	30.654990270418974	25.97831847487637
11	39.5095158404052	19.213461478815404	19.141045886133558	22.13597679464583
12	24.077847436393622	22.618612560396897	26.53916847480895	26.76437152840053
13	32.93304726701935	17.126894503831824	21.657656296987536	28.28240193216129
14	24.98648107417811	18.698461202368545	21.88191538568383	34.43314233776952
15	28.42156932815321	24.75750216100535	19.427876362175056	27.39305214866638
16	28.464182600569345	22.956417140784275	21.39820080290957	27.18119945573681
17	26.919046919100857	23.337778958483074	22.498189610182955	27.24498451223311
18	27.204663725991264	22.014744731664493	23.408576325630133	27.372015216714114
19	27.551638251075783	22.698849576496894	22.828847027791674	26.92066514463565
20	27.83779446647778	22.09633026904348	22.787717128782432	27.2781581356963
21	28.389474521713215	22.42078448876884	22.199626999014228	26.990113990503712
22	28.392845824910694	22.06909013920785	21.97361483265525	27.564449203226204
23	27.630796450152584	22.086620915834743	22.332591197122795	27.949991436889874
24	27.718180629031234	22.720560769088653	22.039827227453735	27.521431374426374
25	27.835367128175598	22.03659077638416	22.18560237771272	27.942439717727524
26	27.69566032367208	22.615645813583114	22.402984007886154	27.285709854858652
27	27.586295247945863	22.197739069223644	22.39394891531691	27.822016767513585
28	27.204394021735467	22.272447148079774	22.92809819392545	27.595060636259312
29	26.80631054017717	22.526778261297576	23.216816599757536	27.45009459876772
30	26.622372237722725	22.904768775798896	23.156807402842414	27.316051583635964
31	27.57645104260923	22.5068201463685	22.20151492880482	27.715213882217455
32	26.87521997753364	22.381812223805987	22.662034945580423	28.080932853079954
33	26.807254505072464	22.16766704470213	23.138602365576027	27.886476084649374
34	27.52358900847276	21.86721650374282	23.059444166499222	27.549750321285195
35	27.015331338420857	21.83067157708215	23.70255396445028	27.451443120046715
36	26.40971043202576	22.25033139910431	23.53102205776256	27.808936111107364
37	26.63464378136155	21.138475604575802	24.446128597686208	27.780752016376443
38	25.825800718222435	20.990542820270434	25.350986375889516	27.832670085617618
39	25.736663461681093	20.739178453866415	25.809213906490836	27.714944177961655
40	25.227866383117593	20.69508180804339	26.498443132183404	27.578608676655612
41	24.659599516150564	20.885088456253296	27.138046774809084	27.317265252787053
42	24.910829030426683	20.942130906354635	27.159218558889247	26.98782150432943
43	23.790882108224228	20.44236892036038	27.59168933306183	28.17505963835356
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	9.0
2	11.0
3	26.5
4	42.0
5	42.0
6	59.0
7	76.0
8	71.5
9	67.0
10	94.5
11	122.0
12	122.0
13	226.0
14	330.0
15	496.5
16	663.0
17	673.5
18	684.0
19	684.0
20	892.5
21	1101.0
22	1194.0
23	1287.0
24	1712.5
25	2138.0
26	2138.0
27	2704.5
28	3271.0
29	4298.0
30	5325.0
31	6900.5
32	8476.0
33	8476.0
34	10705.0
35	12934.0
36	15658.5
37	18383.0
38	21612.0
39	24841.0
40	24841.0
41	27448.0
42	30055.0
43	33218.5
44	36382.0
45	39739.5
46	43097.0
47	43097.0
48	46878.5
49	50660.0
50	53355.5
51	56051.0
52	57916.0
53	59781.0
54	59781.0
55	60473.5
56	61166.0
57	61490.0
58	61814.0
59	60778.0
60	59742.0
61	59742.0
62	56509.0
63	53276.0
64	49199.5
65	45123.0
66	39777.0
67	34431.0
68	34431.0
69	29983.0
70	25535.0
71	21850.0
72	18165.0
73	14388.5
74	10612.0
75	10612.0
76	8583.5
77	6555.0
78	5303.5
79	4052.0
80	3355.0
81	2658.0
82	2658.0
83	1960.0
84	1262.0
85	1019.5
86	777.0
87	578.0
88	379.0
89	379.0
90	281.0
91	183.0
92	107.5
93	32.0
94	17.5
95	3.0
96	3.0
97	3.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	741553.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.33001664916513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.83284055587717	61.772851386383074
2	7.149921987267595	10.057082648095236
3	2.066749811273275	4.360636459095249
4	0.928779529505812	2.6128431909419003
5	0.5050345625153159	1.7759544595052996
6	0.3228245098956174	1.3622551893430408
7	0.22259965878256616	1.0958806395793448
8	0.14757731949415834	0.8303292269650655
9	0.11903956314182178	0.7534849011906302
>10	0.6126615960112214	8.013101092411903
>50	0.05522145739547034	2.774484085399164
>100	0.03655704334905336	4.510582026835841
>500	1.9240549102647646E-4	0.08051469425433315
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	1.3485212789915218E-4	0.0	1.3485212789915218E-4	0.0
11	0.0	1.3485212789915218E-4	0.0	1.3485212789915218E-4	0.0
12	0.0	1.3485212789915218E-4	0.0	1.3485212789915218E-4	0.0
13	0.0	1.3485212789915218E-4	0.0	1.3485212789915218E-4	0.0
14	0.0	1.3485212789915218E-4	0.0	2.6970425579830436E-4	0.0
15	0.0	1.3485212789915218E-4	0.0	4.0455638369745655E-4	0.0
16	0.0	1.3485212789915218E-4	0.0	0.0010788170231932175	0.0
17	0.0	1.3485212789915218E-4	0.0	0.0017530776626889784	0.0
18	0.0	1.3485212789915218E-4	0.0	0.0018879297905881305	0.0
19	0.0	1.3485212789915218E-4	0.0	0.0024273383021847393	0.0
20	0.0	1.3485212789915218E-4	0.0	0.002966746813781348	0.0
21	0.0	1.3485212789915218E-4	0.0	0.0031015989416805	0.0
22	0.0	1.3485212789915218E-4	0.0	0.003775859581176261	0.0
23	0.0	1.3485212789915218E-4	0.0	0.00431526809277287	0.0
24	0.0	1.3485212789915218E-4	0.0	0.006068345755461849	0.0
25	0.0	1.3485212789915218E-4	0.0	0.007551719162352522	0.0
26	0.0	1.3485212789915218E-4	0.0	0.012136691510923697	0.0
27	0.0	1.3485212789915218E-4	0.0	0.034387292614283804	0.0
28	0.0	1.3485212789915218E-4	0.0	0.1074771459356243	0.0
29	0.0	1.3485212789915218E-4	0.0	0.18919753544251053	0.0
30	0.0	1.3485212789915218E-4	0.0	0.30072024521510937	0.0
31	0.0	1.3485212789915218E-4	0.0	0.5988783000001349	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	465	0.0	19.096775	1
ATACAGT	50	0.007032586	18.5	6
CTTATAC	550	0.0	17.154547	37
GTATCAA	540	0.0	16.444445	2
TATGTAC	80	3.3805406E-4	16.1875	3
CATTAGG	70	0.0025913313	15.857142	2
TACATCG	70	0.0025913313	15.857142	5
GTGTACA	70	0.0025913313	15.857142	1
GGGTAAC	245	0.0	15.102041	25
GTCATAC	75	0.0041025802	14.8	1
TTAATTG	75	0.0041025802	14.8	3
GTGATTA	75	0.0041025802	14.8	14
CGGGTAA	250	0.0	14.799999	24
GCGGGTA	240	0.0	14.645833	23
GGTAACC	255	0.0	14.509804	26
TAACCCG	230	1.8189894E-12	14.47826	28
ATACTCT	90	8.269137E-4	14.388887	6
TCGTCAC	155	1.2079181E-7	14.322581	19
ACCCGTT	235	3.6379788E-12	14.170214	30
GTAACCC	250	0.0	14.059999	27
>>END_MODULE