FastQCFastQC Report
Fri 10 Feb 2017
ERR1632179.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632179.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113216
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37870.1792055331778673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32600.15426724007389683No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27510.1301807292770829No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA22760.10770314061600897No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC21850.10339690784094008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14900.023.3422811
TACGTTA801.6177168E-518.519
AATTACG1005.8824844E-616.65000216
CTTATAC14500.016.58620637
TCTTATA25650.016.15594537
AACCGTG700.002593458615.8571427
TCTATCG855.3667935E-415.23529431
GTATTAA4350.014.8850581
ACGCTAA1001.09405315E-414.834
AGTCGGT2900.014.67241511
TCGCCAT8100.014.38888913
AGAGGTC9000.014.3888898
CTAGACT1051.6566683E-414.0952384
AACGCTA1051.6566683E-414.09523833
ATTATAC1854.9039954E-914.03
GTATCAA24550.013.9409372
ATTACGT1203.3029333E-513.87500117
CAGTCGG3200.013.87510
ACTAGGG1356.5727345E-613.7037043
ATAGTAC2304.0017767E-1113.6739123
Produced by FastQC (version 0.11.5)