##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632179.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2113216
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.781250473212395	34.0	31.0	34.0	31.0	34.0
2	32.93603588085648	34.0	31.0	34.0	31.0	34.0
3	32.935415972621826	34.0	33.0	34.0	31.0	34.0
4	36.38622601759593	37.0	37.0	37.0	35.0	37.0
5	36.304634263605806	37.0	37.0	37.0	35.0	37.0
6	36.336846304400495	37.0	37.0	37.0	35.0	37.0
7	36.32512861912838	37.0	37.0	37.0	35.0	37.0
8	36.311453254186986	37.0	37.0	37.0	35.0	37.0
9	38.09167354402011	39.0	39.0	39.0	37.0	39.0
10	38.030509422605164	39.0	38.0	39.0	35.0	39.0
11	38.1376470744117	39.0	39.0	39.0	37.0	39.0
12	38.112620290590264	39.0	38.0	39.0	37.0	39.0
13	38.13030234486205	39.0	39.0	39.0	37.0	39.0
14	39.53651401465823	41.0	39.0	41.0	37.0	41.0
15	39.565040677337294	41.0	39.0	41.0	37.0	41.0
16	39.44183983085496	41.0	39.0	41.0	37.0	41.0
17	39.49268224355674	41.0	39.0	41.0	37.0	41.0
18	39.50967530058451	41.0	39.0	41.0	37.0	41.0
19	39.572083970592686	41.0	39.0	41.0	37.0	41.0
20	39.55538525167328	41.0	39.0	41.0	37.0	41.0
21	39.525433273266906	41.0	39.0	41.0	37.0	41.0
22	39.50948696205215	41.0	39.0	41.0	37.0	41.0
23	39.46910207002029	41.0	39.0	41.0	37.0	41.0
24	39.41742538386989	41.0	39.0	41.0	37.0	41.0
25	39.357950157485085	41.0	39.0	41.0	36.0	41.0
26	39.27840410066931	40.0	39.0	41.0	36.0	41.0
27	39.21223339213786	40.0	39.0	41.0	36.0	41.0
28	39.14933352766589	40.0	39.0	41.0	36.0	41.0
29	39.01255953011902	40.0	39.0	41.0	35.0	41.0
30	38.96709044413822	40.0	39.0	41.0	35.0	41.0
31	38.89394931705988	40.0	38.0	41.0	35.0	41.0
32	38.82771945697932	40.0	38.0	41.0	35.0	41.0
33	38.69111297661952	40.0	38.0	41.0	35.0	41.0
34	38.69444675792725	40.0	38.0	41.0	35.0	41.0
35	38.667267804143066	40.0	38.0	41.0	35.0	41.0
36	38.63680759562676	40.0	38.0	41.0	35.0	41.0
37	38.610726967806414	40.0	38.0	41.0	35.0	41.0
38	38.53874095217905	40.0	38.0	41.0	35.0	41.0
39	38.360360701414336	40.0	38.0	41.0	34.0	41.0
40	38.36809251870135	40.0	38.0	41.0	34.0	41.0
41	38.366293838396075	40.0	38.0	41.0	34.0	41.0
42	38.23743526454466	40.0	38.0	41.0	34.0	41.0
43	37.39893839531785	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	1.0
12	0.0
13	2.0
14	4.0
15	4.0
16	7.0
17	11.0
18	44.0
19	62.0
20	131.0
21	298.0
22	478.0
23	801.0
24	1284.0
25	1943.0
26	2895.0
27	4246.0
28	5957.0
29	8729.0
30	12356.0
31	17238.0
32	23409.0
33	32972.0
34	49356.0
35	73416.0
36	118918.0
37	221388.0
38	517979.0
39	1019282.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.853593764196376	17.85657500227142	12.487980405221236	26.801850828310975
2	18.9805017565644	20.257702004906267	34.15893122141797	26.60286501711136
3	20.15387920591175	21.34859853417729	29.950416805475633	28.54710545443532
4	15.35716178563857	15.376374208788878	33.26844014052515	35.998023865047394
5	15.827014370513947	35.046535706714316	33.34661482782641	15.779835094945335
6	35.350905917804894	33.68046617099246	15.556005633120323	15.412622278082317
7	30.234770132348043	29.71404721523971	20.290306338774645	19.760876313637603
8	27.886406311517607	32.70299865229111	19.72860322844423	19.681991807747053
9	27.13556020775917	14.259592961628154	18.817811335897513	39.78703549471516
10	17.650916896332415	26.317281338017505	32.09525197613495	23.936549789515126
11	36.669370286804565	20.91825918410612	20.85697817923014	21.555392349859172
12	22.763408946364212	24.6239854326297	27.82044050395227	24.79216511705382
13	31.508042717829127	18.670121748084437	23.761697810351613	26.060137723734822
14	23.78455396892698	19.86621339228929	23.851182273842333	32.4980503649414
15	26.54276704321754	26.445096005330264	21.297870165662193	25.714266785790002
16	26.250085178230716	25.150386898452403	22.877642418001756	25.72188550531512
17	25.009227641660864	25.468669553893214	24.17670507889397	25.345397725551955
18	24.564076743693025	24.260747599866743	25.482345392047	25.69283026439323
19	25.81283692722372	24.440331702958904	25.421868848238894	24.324962521578485
20	26.131545473818107	23.813798494806022	25.256102547018383	24.79855348435749
21	26.18047563524032	24.472604788152275	23.936171219600837	25.410748357006575
22	26.403879205911746	24.013683409552076	23.98921832884097	25.593219055695204
23	25.56113525545898	24.327517868499953	24.3882310185045	25.72311585753657
24	26.12714459856446	24.193125548926375	24.282751976134954	25.396977876374212
25	25.896074987128625	23.662843741482178	24.54514824797844	25.895933023410763
26	25.636376025924466	23.974170174747872	24.472131575759413	25.917322223568252
27	25.57589948211636	23.974832672097886	24.371337336079225	26.077930509706533
28	25.08598269178352	24.249674429873707	24.880987083194526	25.78335579514825
29	25.008281216875133	24.725962703292044	24.876444244223023	25.389311835609803
30	24.837262258093826	25.26831142675429	24.59989892183288	25.294527393319
31	25.605049365516823	24.4812645749417	24.571222250825283	25.342463808716193
32	24.466263742087886	24.63723537962991	24.93711007298828	25.959390805293918
33	24.78998834004664	24.120298161664497	25.29424346588328	25.795470032405582
34	25.276639964868714	23.81498152578818	25.116126321209002	25.792252188134107
35	25.02214633998607	24.06554748781005	25.39163057633484	25.520675595869047
36	24.698705669463035	24.065310881613616	25.469615978678945	25.766367470244404
37	25.241906175232444	22.84167827614404	25.635997456010173	26.280418092613345
38	24.20784245434447	23.315553166358765	26.79035176716436	25.68625261213241
39	24.21489331899815	22.803253429843423	27.223104500439142	25.758748750719285
40	23.753369272237197	22.47001726278809	28.0487654835095	25.727847981465217
41	23.00876957206457	22.63748712862291	28.68263348375178	25.67110981556074
42	23.316121021230202	22.115060646900268	28.562153608528423	26.006664723341107
43	22.157649762258096	22.12286865138254	29.111789802840786	26.60769178351858
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	54.0
1	62.0
2	70.0
3	221.5
4	373.0
5	373.0
6	498.5
7	624.0
8	655.5
9	687.0
10	1035.0
11	1383.0
12	1383.0
13	2390.5
14	3398.0
15	5872.5
16	8347.0
17	8353.0
18	8359.0
19	8359.0
20	9720.0
21	11081.0
22	11991.0
23	12901.0
24	15294.5
25	17688.0
26	17688.0
27	20848.0
28	24008.0
29	31770.5
30	39533.0
31	44483.0
32	49433.0
33	49433.0
34	56446.0
35	63459.0
36	70200.0
37	76941.0
38	87583.0
39	98225.0
40	98225.0
41	108549.5
42	118874.0
43	119326.0
44	119778.0
45	127785.0
46	135792.0
47	135792.0
48	143042.0
49	150292.0
50	157881.0
51	165470.0
52	174797.0
53	184124.0
54	184124.0
55	169504.5
56	154885.0
57	147652.0
58	140419.0
59	130184.5
60	119950.0
61	119950.0
62	112112.5
63	104275.0
64	94741.0
65	85207.0
66	75742.5
67	66278.0
68	66278.0
69	57984.0
70	49690.0
71	43275.5
72	36861.0
73	30714.0
74	24567.0
75	24567.0
76	20016.0
77	15465.0
78	13250.0
79	11035.0
80	8679.5
81	6324.0
82	6324.0
83	4966.5
84	3609.0
85	2800.0
86	1991.0
87	1535.0
88	1079.0
89	1079.0
90	789.5
91	500.0
92	310.5
93	121.0
94	84.0
95	47.0
96	47.0
97	31.0
98	15.0
99	9.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2113216.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.557179609718666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.83734906202504	50.26393066247117
2	8.303458632785771	9.724542370838108
3	2.2209822676585214	3.9016337267184094
4	1.0485408527853424	2.455983801787155
5	0.5687526421449846	1.6652275309792952
6	0.3657224447212804	1.2849404929709656
7	0.2544304318040182	1.0429109945328323
8	0.17618157431639653	0.8253356872934584
9	0.14487798775790778	0.7635281715570986
>10	0.8885848076318058	9.913234304286787
>50	0.0973421086097661	3.9877723900935003
>100	0.08616673169328012	10.144493232875783
>500	0.0057483231987047745	2.289060266924397
>1k	0.0018621328671860502	1.737406366671049
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3787	0.1792055331778673	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3260	0.15426724007389683	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2751	0.1301807292770829	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2276	0.10770314061600897	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2185	0.10339690784094008	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.7321239286471425E-5	0.0
10	0.0	0.0	0.0	9.464247857294285E-5	0.0
11	0.0	0.0	0.0	9.464247857294285E-5	0.0
12	0.0	0.0	0.0	1.4196371785941427E-4	0.0
13	0.0	0.0	0.0	1.4196371785941427E-4	0.0
14	0.0	0.0	0.0	1.892849571458857E-4	0.0
15	0.0	0.0	0.0	3.785699142917714E-4	0.0
16	0.0	0.0	0.0	6.151761107241285E-4	0.0
17	0.0	0.0	0.0	8.044610678700142E-4	0.0
18	0.0	0.0	0.0	8.991035464429571E-4	0.0
19	0.0	0.0	0.0	9.937460250159E-4	0.0
20	0.0	0.0	0.0	0.0013249947000212	0.0
21	0.0	0.0	0.0	0.0016562433750264998	0.0
22	0.0	0.0	0.0	0.0025080256821829855	0.0
23	0.0	0.0	0.0	0.0033598079893394713	0.0
24	0.0	0.0	0.0	0.004684802689360671	0.0
25	0.0	0.0	0.0	0.0060571186286683424	0.0
26	0.0	0.0	0.0	0.008849071746570156	0.0
27	0.0	0.0	0.0	0.024370438232532782	0.0
28	0.0	4.7321239286471425E-5	0.0	0.08219699264060086	0.0
29	0.0	4.7321239286471425E-5	0.0	0.16562433750265	0.0
30	0.0	4.7321239286471425E-5	0.0	0.2798104879009055	0.0
31	0.0	4.7321239286471425E-5	0.0	0.6293251612707835	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1490	0.0	23.342281	1
TACGTTA	80	1.6177168E-5	18.5	19
AATTACG	100	5.8824844E-6	16.650002	16
CTTATAC	1450	0.0	16.586206	37
TCTTATA	2565	0.0	16.155945	37
AACCGTG	70	0.0025934586	15.857142	7
TCTATCG	85	5.3667935E-4	15.235294	31
GTATTAA	435	0.0	14.885058	1
ACGCTAA	100	1.09405315E-4	14.8	34
AGTCGGT	290	0.0	14.672415	11
TCGCCAT	810	0.0	14.388889	13
AGAGGTC	900	0.0	14.388889	8
CTAGACT	105	1.6566683E-4	14.095238	4
AACGCTA	105	1.6566683E-4	14.095238	33
ATTATAC	185	4.9039954E-9	14.0	3
GTATCAA	2455	0.0	13.940937	2
ATTACGT	120	3.3029333E-5	13.875001	17
CAGTCGG	320	0.0	13.875	10
ACTAGGG	135	6.5727345E-6	13.703704	3
ATAGTAC	230	4.0017767E-11	13.673912	3
>>END_MODULE