FastQCFastQC Report
Fri 10 Feb 2017
ERR1632178.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632178.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473093
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30210.20507870175202786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24830.16855690713349394No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20500.13916297205946943No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA14860.10087618364896173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11950.026.163181
CGAACGA1551.8189894E-1219.09677316
ACTGCGT500.007035799318.58
GTACTAT1501.2732926E-1118.51
TATACGT500.007035799318.54
ATAACGA1653.6379788E-1217.93939412
CTTATAC9000.017.67777837
CTACTAG650.001580219817.0769231
CGTCGGA650.001580219817.07692312
TAACGAA1851.8189894E-1217.013
GTATCAA18650.016.7640742
TACTCCG1005.8800506E-616.6500025
TCGAACG1751.3096724E-1015.8571433
TACTATA1455.3481926E-815.3103452
ACGAACG1954.1836756E-1115.17948715
TATACAG1101.4523415E-515.1363645
TCTTATA15650.014.89456937
TCCGATA2251.8189894E-1214.88
GCAATAC1508.105053E-814.837
CGCAATA1508.105053E-814.836
Produced by FastQC (version 0.11.5)