FastQCFastQC Report
Fri 10 Feb 2017
ERR1632177.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632177.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64107
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAAAG250.0054581929.63
GGTATCA400.001911470923.1250021
CTTATAC1151.2732926E-1020.91304437
TCTTATA1751.2187229E-1015.85714237
CTCTTAT2652.910383E-1112.56603737
TCTCTTA2951.8735591E-1011.28813636
TGTCTCT5650.08.84070837
GTCTCTT4003.8235157E-98.787537
GCTGTCT2452.8274782E-48.30612336
CTGTCTC6850.08.1021937
CCTGTCT3851.0044106E-46.727272536