FastQCFastQC Report
Fri 10 Feb 2017
ERR1632176.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632176.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139071
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA3420.24591755290463146No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC3020.21715526601520088No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG2840.2042122369149571No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG2320.16682126395869734No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA2290.16466409244199007No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA1980.14237332010268136No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT1870.13446369120808796No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC1840.13230651969138066No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA1780.12799217665796608No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT1710.12295877645231573No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC1580.11361103321325078No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG1550.11145386169654349No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA1530.11001574735207197No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA1530.11001574735207197No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC1520.1092966901798362No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA1490.10713951866312892No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC1480.10642046149089315No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC1450.10426328997418584No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT1420.10210611845747855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGATCG200.001836378237.016
CCGGAGG250.00547994929.59999828
GGTATCA604.26553E-827.7499981
AAAGTAA358.8300795E-426.42857217
TGAAGGT400.001922708723.1254
CAGGCTT400.001922708723.1254
CAAAGTA400.001922708723.12516
ACTATTC502.6855865E-422.1999998
TTCCTCG1005.2204996E-1022.19999919
CTTTCAC1107.2759576E-1121.86363628
GAAATCT855.0986273E-821.7647068
TCGCCAT450.00380917120.55555713
TTGCTTT450.00380917120.55555720
GCCACAA450.00380917120.55555712
AAATCTC1001.2654709E-820.3499989
ATCTCGC1101.715307E-920.18181811
CTATATC555.1119126E-420.1818181
AATCTCG1101.715307E-920.18181810
TGTAAAC555.1119126E-420.1818182
GGCCTTT1202.3283064E-1020.04166625
Produced by FastQC (version 0.11.5)