##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632176.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	139071
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78233420339251	34.0	31.0	34.0	31.0	34.0
2	32.943438962831934	34.0	33.0	34.0	31.0	34.0
3	32.946437431240156	34.0	33.0	34.0	31.0	34.0
4	36.39200120801605	37.0	37.0	37.0	35.0	37.0
5	36.31555104946394	37.0	37.0	37.0	35.0	37.0
6	36.35442328019501	37.0	37.0	37.0	35.0	37.0
7	36.33917207757189	37.0	37.0	37.0	35.0	37.0
8	36.32381301637293	37.0	37.0	37.0	35.0	37.0
9	38.1181626651135	39.0	39.0	39.0	37.0	39.0
10	38.064441903775766	39.0	38.0	39.0	37.0	39.0
11	38.16961120578697	39.0	39.0	39.0	37.0	39.0
12	38.134291117486754	39.0	38.0	39.0	37.0	39.0
13	38.14413501017466	39.0	39.0	39.0	37.0	39.0
14	39.563884634467286	41.0	39.0	41.0	37.0	41.0
15	39.59379022226057	41.0	39.0	41.0	37.0	41.0
16	39.50331844884987	41.0	39.0	41.0	37.0	41.0
17	39.55316349202925	41.0	39.0	41.0	37.0	41.0
18	39.55903818912642	41.0	39.0	41.0	37.0	41.0
19	39.61669219319628	41.0	39.0	41.0	37.0	41.0
20	39.60246205175773	41.0	39.0	41.0	37.0	41.0
21	39.566904674590674	41.0	39.0	41.0	37.0	41.0
22	39.56229551811665	41.0	39.0	41.0	37.0	41.0
23	39.54236325330227	41.0	39.0	41.0	37.0	41.0
24	39.50577043380719	41.0	39.0	41.0	37.0	41.0
25	39.44585859021651	41.0	39.0	41.0	37.0	41.0
26	39.37760568342789	41.0	39.0	41.0	37.0	41.0
27	39.33121930524696	40.0	39.0	41.0	36.0	41.0
28	39.26855347268661	40.0	39.0	41.0	36.0	41.0
29	39.1658577273479	40.0	39.0	41.0	36.0	41.0
30	39.11640816561325	40.0	39.0	41.0	36.0	41.0
31	39.066656599866256	40.0	39.0	41.0	36.0	41.0
32	39.01791890473211	40.0	39.0	41.0	35.0	41.0
33	38.91484924966384	40.0	38.0	41.0	35.0	41.0
34	38.92465718949314	40.0	38.0	41.0	35.0	41.0
35	38.90786720452143	40.0	38.0	41.0	35.0	41.0
36	38.89063859467466	40.0	38.0	41.0	35.0	41.0
37	38.88826570600629	40.0	38.0	41.0	35.0	41.0
38	38.83445865780788	40.0	38.0	41.0	35.0	41.0
39	38.69338683118695	40.0	38.0	41.0	35.0	41.0
40	38.7128301371242	40.0	38.0	41.0	35.0	41.0
41	38.72570126050722	40.0	38.0	41.0	35.0	41.0
42	38.65112065060293	40.0	38.0	41.0	35.0	41.0
43	37.83871547626752	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	2.0
20	7.0
21	11.0
22	21.0
23	39.0
24	53.0
25	94.0
26	146.0
27	205.0
28	318.0
29	484.0
30	690.0
31	970.0
32	1395.0
33	1901.0
34	2953.0
35	4430.0
36	7109.0
37	13400.0
38	34249.0
39	70592.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.1669506942497	18.21299911555968	12.927928899626808	26.692121290563815
2	18.06343522373464	20.577259097870872	35.701188601505706	25.658117076888782
3	19.19019781262808	21.535762308461145	31.37534065333535	27.898699225575424
4	15.123210446462599	15.42161917294044	33.93015078628902	35.52501959430794
5	14.94488426774813	36.26349131019408	34.30693674454056	14.484687677517238
6	35.1662100653623	34.59887395646828	15.679760697773078	14.555155280396345
7	29.68052289837565	30.854743260636653	20.483781665480223	18.980952175507472
8	27.18611356788978	34.56939261240662	19.91716461375844	18.327329205945166
9	27.003473046141902	14.876573836385731	18.359686778695774	39.760266338776596
10	16.52681004666681	27.421964320383115	32.75880665271696	23.29241898023312
11	36.50868980592647	21.17551466517103	21.507000021571717	20.80879550733079
12	22.605000323575727	24.877220987840744	28.074149175600954	24.443629512982575
13	30.825980973747225	18.799030710931827	24.350871137764162	26.02411717755679
14	23.029963112367064	19.68778537581523	24.162478158638393	33.11977335317931
15	25.845790998842315	27.163822795550473	21.7982181763272	25.19216802928001
16	26.065822493546463	25.539472643469885	22.644548468048693	25.750156394934958
17	25.106600225783954	25.759504138174027	23.912965319872583	25.220930316169436
18	23.856159803265957	24.921083475347125	25.410042352467443	25.81271436891947
19	25.881743857454104	24.74491446814936	25.659555191233256	23.713786483163275
20	25.920572944754838	24.26817956295705	25.316564920076797	24.494682572211314
21	25.76166130969073	25.49848638465244	23.204694005220354	25.535158300436468
22	25.82278116933077	24.657908550308836	23.81014014424287	25.70917013611752
23	24.90094987452452	25.215177858791556	24.243012561928797	25.640859704755126
24	26.6094297157567	24.511220887172737	24.081224698175753	24.798124698894807
25	25.950773345988736	23.930222692006243	24.57521697550172	25.543786986503296
26	25.87742951442069	23.770591999769902	24.225036132622904	26.1269423531865
27	25.169877256940698	23.948918178484373	24.568026403779363	26.313178160795562
28	24.793810355861396	24.772957697866556	24.790934127172452	25.642297819099596
29	25.12026231205643	25.327350777660328	24.3515901949364	25.200796715346836
30	24.65862760748107	25.7026986215674	24.196273845733472	25.442399925218055
31	25.51790092830281	24.595350576324325	24.827606042956475	25.059142452416395
32	24.118615671132012	25.34245097827728	24.89160213128546	25.647331219305247
33	24.863558901568265	24.22072178958949	25.225963716375087	25.689755592467158
34	24.921083475347125	24.278246363368353	25.371213265166716	25.42945689611781
35	24.78805789848351	24.41918156912656	24.91533101796924	25.87742951442069
36	24.752824097043955	24.401205139820668	25.23243523092521	25.613535532210168
37	25.465409754729603	22.807055388973975	25.24250203133651	26.485032824959916
38	24.03808126784161	23.470745158947587	26.836651782183203	25.654521791027605
39	24.13371587174896	22.630886381776214	27.56721386917474	25.668183877300084
40	23.375110555040234	22.211676050362765	28.78098237590871	25.632231018688294
41	22.67762509797154	22.374902028460284	29.275693710406912	25.67177916316126
42	22.90700433591475	21.385479359463872	29.20666422187228	26.5008520827491
43	21.548705337561387	21.433656190003667	30.25576863616426	26.76186983627068
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	3.0
4	5.0
5	5.0
6	7.5
7	10.0
8	10.0
9	10.0
10	21.5
11	33.0
12	33.0
13	56.5
14	80.0
15	145.0
16	210.0
17	245.5
18	281.0
19	281.0
20	322.5
21	364.0
22	497.5
23	631.0
24	804.0
25	977.0
26	977.0
27	1177.0
28	1377.0
29	2044.0
30	2711.0
31	3034.5
32	3358.0
33	3358.0
34	3815.5
35	4273.0
36	4694.5
37	5116.0
38	6033.0
39	6950.0
40	6950.0
41	7984.5
42	9019.0
43	8789.0
44	8559.0
45	9255.5
46	9952.0
47	9952.0
48	10423.5
49	10895.0
50	11572.5
51	12250.0
52	13053.5
53	13857.0
54	13857.0
55	12114.5
56	10372.0
57	9604.5
58	8837.0
59	7982.0
60	7127.0
61	7127.0
62	6651.5
63	6176.0
64	5454.5
65	4733.0
66	4184.0
67	3635.0
68	3635.0
69	3139.0
70	2643.0
71	2236.5
72	1830.0
73	1496.0
74	1162.0
75	1162.0
76	929.0
77	696.0
78	562.0
79	428.0
80	333.5
81	239.0
82	239.0
83	186.0
84	133.0
85	107.5
86	82.0
87	59.5
88	37.0
89	37.0
90	25.0
91	13.0
92	9.0
93	5.0
94	3.0
95	1.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	139071.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.55913166655881
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.65158061858156	66.30066656599867
2	3.8565887578126565	5.519482854081728
3	1.0882453425510963	2.336216752593999
4	0.6099399103679736	1.7458708141884363
5	0.3496854840330393	1.2511594796902301
6	0.22006069253803331	0.9448411243177945
7	0.15675556180791414	0.7852104320814547
8	0.1276151048051609	0.7305620869915367
9	0.09043590104302739	0.5824363095109691
>10	0.7104242448602263	10.514773029603584
>50	0.09847464780240761	4.819840225496329
>100	0.04019373379690106	4.468940325445276
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	342	0.24591755290463146	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	302	0.21715526601520088	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	284	0.2042122369149571	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	232	0.16682126395869734	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	229	0.16466409244199007	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	198	0.14237332010268136	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	187	0.13446369120808796	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	184	0.13230651969138066	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	178	0.12799217665796608	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	171	0.12295877645231573	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	158	0.11361103321325078	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	155	0.11145386169654349	No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA	153	0.11001574735207197	No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA	153	0.11001574735207197	No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC	152	0.1092966901798362	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	149	0.10713951866312892	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	148	0.10642046149089315	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	145	0.10426328997418584	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	142	0.10210611845747855	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	7.190571722357644E-4	0.0
10	0.0	0.0	0.0	0.005033400205650351	0.0
11	0.0	0.0	0.0	0.005033400205650351	0.0
12	0.0	0.0	0.0	0.005033400205650351	0.0
13	0.0	0.0	0.0	0.005033400205650351	0.0
14	0.0	0.0	0.0	0.005033400205650351	0.0
15	0.0	0.0	0.0	0.005033400205650351	0.0
16	0.0	0.0	0.0	0.0057524573778861155	0.0
17	0.0	0.0	0.0	0.0057524573778861155	0.0
18	0.0	0.0	0.0	0.0057524573778861155	0.0
19	0.0	0.0	0.0	0.0057524573778861155	0.0
20	0.0	0.0	0.0	0.006471514550121881	0.0
21	0.0	0.0	0.0	0.007190571722357645	0.0
22	0.0	0.0	0.0	0.00790962889459341	0.0
23	0.0	0.0	0.0	0.010066800411300703	0.0
24	0.0	0.0	0.0	0.010785857583536467	0.0
25	0.0	0.0	0.0	0.012943029100243761	0.0
26	0.0	0.0	0.0	0.012943029100243761	0.0
27	0.0	0.0	0.0	0.028043229717194813	0.0
28	0.0	0.0	0.0	0.0870059178405275	0.0
29	0.0	0.0	0.0	0.18695486478129877	0.0
30	0.0	0.0	0.0	0.2883419260665416	0.0
31	0.0	0.0	0.0	0.7017998001021061	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGATCG	20	0.0018363782	37.0	16
CCGGAGG	25	0.005479949	29.599998	28
GGTATCA	60	4.26553E-8	27.749998	1
AAAGTAA	35	8.8300795E-4	26.428572	17
TGAAGGT	40	0.0019227087	23.125	4
CAGGCTT	40	0.0019227087	23.125	4
CAAAGTA	40	0.0019227087	23.125	16
ACTATTC	50	2.6855865E-4	22.199999	8
TTCCTCG	100	5.2204996E-10	22.199999	19
CTTTCAC	110	7.2759576E-11	21.863636	28
GAAATCT	85	5.0986273E-8	21.764706	8
TCGCCAT	45	0.003809171	20.555557	13
TTGCTTT	45	0.003809171	20.555557	20
GCCACAA	45	0.003809171	20.555557	12
AAATCTC	100	1.2654709E-8	20.349998	9
ATCTCGC	110	1.715307E-9	20.181818	11
CTATATC	55	5.1119126E-4	20.181818	1
AATCTCG	110	1.715307E-9	20.181818	10
TGTAAAC	55	5.1119126E-4	20.181818	2
GGCCTTT	120	2.3283064E-10	20.041666	25
>>END_MODULE