FastQCFastQC Report
Fri 10 Feb 2017
ERR1632175.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632175.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543222
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7700.14174683646833156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7060.1299652812294053No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT5560.10235226113817188RNA PCR Primer, Index 29 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3050.031.5409851
TAGGACG250.005493822529.64
TAGACTT551.9002646E-523.5454545
CTTAGAC450.0038232520.5555553
GCCGTCT951.6720514E-719.47368436
ATTAGAC500.007030221618.53
TTAGACT609.22827E-418.54
CCGTCTT1002.8680006E-718.537
GTTAATG500.007030221618.53
GTATCAA5350.017.9813082
TAGACAG852.7188356E-517.4117645
CTTATAC5100.017.41176437
GTGTAGA852.7188356E-517.4117641
TATATCC1005.8663154E-616.6500022
CTCTTAT14850.015.94612837
TCTTATA8400.015.85714237
GTCTCGC855.357684E-415.2352941
CTTAAGC750.00410070214.83
GTCCTAA1001.0916781E-414.81
CTGACCG750.00410070214.89
Produced by FastQC (version 0.11.5)