##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632175.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	543222
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71393463445884	34.0	31.0	34.0	31.0	34.0
2	32.87520571699968	34.0	31.0	34.0	31.0	34.0
3	32.88082588702225	34.0	31.0	34.0	31.0	34.0
4	36.34705516345067	37.0	37.0	37.0	35.0	37.0
5	36.26028400911598	37.0	37.0	37.0	35.0	37.0
6	36.29110934387783	37.0	37.0	37.0	35.0	37.0
7	36.281481604206014	37.0	37.0	37.0	35.0	37.0
8	36.26622080843559	37.0	37.0	37.0	35.0	37.0
9	38.032078965873986	39.0	38.0	39.0	35.0	39.0
10	37.966001008795665	39.0	38.0	39.0	35.0	39.0
11	38.08022686857307	39.0	38.0	39.0	37.0	39.0
12	38.04721458261999	39.0	38.0	39.0	35.0	39.0
13	38.0685539245465	39.0	38.0	39.0	35.0	39.0
14	39.45551542463302	40.0	39.0	41.0	37.0	41.0
15	39.48049600347556	40.0	39.0	41.0	37.0	41.0
16	39.369487980972785	40.0	39.0	41.0	37.0	41.0
17	39.419898310451345	40.0	39.0	41.0	37.0	41.0
18	39.4475499887707	40.0	39.0	41.0	37.0	41.0
19	39.51235958779284	41.0	39.0	41.0	37.0	41.0
20	39.49450684986985	41.0	39.0	41.0	37.0	41.0
21	39.47199119328746	40.0	39.0	41.0	37.0	41.0
22	39.4487281442946	40.0	39.0	41.0	37.0	41.0
23	39.418839811347844	40.0	39.0	41.0	37.0	41.0
24	39.374450961117184	40.0	39.0	41.0	37.0	41.0
25	39.316218783480785	40.0	39.0	41.0	36.0	41.0
26	39.23830404512336	40.0	39.0	41.0	36.0	41.0
27	39.178626786102186	40.0	39.0	41.0	36.0	41.0
28	39.141297664674845	40.0	39.0	41.0	36.0	41.0
29	39.007275110360034	40.0	39.0	41.0	36.0	41.0
30	38.97383206129354	40.0	39.0	41.0	35.0	41.0
31	38.90722577509747	40.0	38.0	41.0	35.0	41.0
32	38.84661887773323	40.0	38.0	41.0	35.0	41.0
33	38.729791871463235	40.0	38.0	41.0	35.0	41.0
34	38.74064931096311	40.0	38.0	41.0	35.0	41.0
35	38.72924881540144	40.0	38.0	41.0	35.0	41.0
36	38.716460305363185	40.0	38.0	41.0	35.0	41.0
37	38.7003950502741	40.0	38.0	41.0	35.0	41.0
38	38.64752900287544	40.0	38.0	41.0	35.0	41.0
39	38.48032480275099	40.0	38.0	41.0	35.0	41.0
40	38.500498875229646	40.0	38.0	41.0	35.0	41.0
41	38.52747679585878	40.0	38.0	41.0	35.0	41.0
42	38.42267249853651	40.0	38.0	41.0	35.0	41.0
43	37.62029520159346	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.0
17	3.0
18	7.0
19	6.0
20	22.0
21	59.0
22	96.0
23	171.0
24	289.0
25	440.0
26	735.0
27	1074.0
28	1583.0
29	2268.0
30	3315.0
31	4480.0
32	6082.0
33	8846.0
34	12605.0
35	18841.0
36	29915.0
37	56061.0
38	137308.0
39	259012.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.667679144070014	18.994444260357643	13.084889787232475	26.252986808339866
2	18.59350320863293	20.54353468747584	35.23899989322965	25.62396221066157
3	19.62549381284263	21.98327755503275	30.463051938249926	27.928176693874697
4	15.078365751018922	16.037089808586543	34.403429905268936	34.4811145351256
5	15.445434831431717	35.130756854472025	34.1587785472606	15.265029766835658
6	34.51958867645272	34.49584147917426	15.854659789183795	15.129910055189221
7	29.104123176160023	30.098560073045643	21.196858742834422	19.600458007959915
8	26.759041423211873	33.44194454569219	20.42443052748232	19.374583503613625
9	26.321651184966736	14.674663397285087	19.950222929115537	39.05346248863264
10	17.001704643773632	26.779659144879993	33.60467727742986	22.61395893391652
11	35.4302660790616	21.509438130267185	21.576629812489184	21.48366597818203
12	21.75316905427247	25.49141971422365	28.998457352610902	23.756953878892975
13	31.037402756147582	19.794117322199764	23.997003066886098	25.17147685476656
14	23.266730728873277	20.963988940065022	24.700582818810727	31.068697512250974
15	25.690969806082965	27.382175243270705	22.205286236566266	24.721568714080064
16	25.130609585031532	26.183770171311178	23.66141282937731	25.02420741427998
17	23.50475496205971	26.43688952214748	25.3717632938283	24.686592221964503
18	23.614102521620993	25.195776312446842	26.39602225241246	24.7940989135197
19	24.50121681375202	25.49749457864372	26.47904539948677	23.52224320811749
20	25.2955513583765	25.064338336812575	26.074238524949283	23.56587177986164
21	25.209398735691853	25.30788517401725	24.861474682542312	24.62124140774858
22	25.358509044184512	25.213816818906448	25.122325679004163	24.30534845790487
23	25.001196564203955	25.16429746954284	25.075751718450284	24.758754247802926
24	25.07280632964055	25.208478301688814	25.121221158200512	24.59749421047012
25	24.976528932922452	24.963458770079267	25.44687070847646	24.61314158852182
26	24.481335439286333	25.023471067077548	25.292421882766163	25.202771610869956
27	24.180721693893105	25.339364016921255	25.666486261602074	24.813428027583566
28	24.239445383287126	25.14588878948202	25.709378486143784	24.90528734108707
29	24.114266358873536	25.46178173932573	25.909112664803708	24.51483923699703
30	24.575403794397136	25.753007057887935	25.309541955222727	24.362047192492202
31	24.612957501721212	25.44595027447342	25.320955336860436	24.620136886944934
32	23.689762196670973	25.503937616665013	25.907271796797627	24.89902838986639
33	23.97288769600642	24.886142313823814	26.211935451804237	24.92903453836553
34	24.226375220443945	24.14187937896477	26.426764748114028	25.204980652477253
35	23.946379196718837	24.25969493135403	26.72940344831395	25.064522423613184
36	24.024616086977332	24.302587155895747	26.589865653452915	25.082931103674007
37	23.93551807548295	23.19751409184459	27.01326529485183	25.853702537820634
38	23.163089860130846	23.39780053090633	28.25382624415064	25.18528336481218
39	23.012138683632106	22.924145192941374	28.86425807496751	25.19945804845901
40	22.355501065862576	22.512158933180174	30.009277974750653	25.1230620262066
41	21.924553865638728	22.478655135469477	30.053642893697237	25.543148105194565
42	21.658548438759844	22.460246455408654	30.390153565209065	25.491051540622433
43	20.898085865447275	22.44828081336912	30.462683764648705	26.1909495565349
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10.0
1	12.0
2	14.0
3	61.5
4	109.0
5	109.0
6	139.5
7	170.0
8	185.0
9	200.0
10	291.5
11	383.0
12	383.0
13	657.5
14	932.0
15	1379.0
16	1826.0
17	1835.5
18	1845.0
19	1845.0
20	2370.0
21	2895.0
22	3317.5
23	3740.0
24	4780.5
25	5821.0
26	5821.0
27	6827.5
28	7834.0
29	9981.0
30	12128.0
31	14018.5
32	15909.0
33	15909.0
34	18102.0
35	20295.0
36	22498.5
37	24702.0
38	27000.0
39	29298.0
40	29298.0
41	31700.0
42	34102.0
43	34483.0
44	34864.0
45	36159.5
46	37455.0
47	37455.0
48	38165.5
49	38876.0
50	40273.0
51	41670.0
52	42488.5
53	43307.0
54	43307.0
55	41470.0
56	39633.0
57	37692.0
58	35751.0
59	32755.0
60	29759.0
61	29759.0
62	26943.0
63	24127.0
64	21147.5
65	18168.0
66	15444.5
67	12721.0
68	12721.0
69	10772.0
70	8823.0
71	7439.0
72	6055.0
73	5031.0
74	4007.0
75	4007.0
76	3216.5
77	2426.0
78	1965.0
79	1504.0
80	1191.5
81	879.0
82	879.0
83	713.0
84	547.0
85	398.5
86	250.0
87	181.5
88	113.0
89	113.0
90	80.0
91	47.0
92	30.0
93	13.0
94	10.0
95	7.0
96	7.0
97	5.5
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	543222.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.09739997027506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.44015128642826	71.53580819674804
2	6.6391367267338115	10.502769062636142
3	1.3078946719605187	3.1035320396115855
4	0.46872694705380874	1.4830033123184423
5	0.26500164814545457	1.048047067807156
6	0.16420929288000144	0.7793116870659309
7	0.11100686062118356	0.6146247837798834
8	0.07955254640068743	0.5033919665047232
9	0.05861930793623091	0.4172971361231498
>10	0.4181385222283385	6.2553345135521505
>50	0.0340396196547887	1.7925042810485938
>100	0.012823126683315488	1.5896470815981094
>500	6.994432736318615E-4	0.3747288712060903
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	770	0.14174683646833156	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	706	0.1299652812294053	No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	556	0.10235226113817188	RNA PCR Primer, Index 29 (95% over 23bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.8408680060822278E-4	0.0
10	0.0	0.0	0.0	1.8408680060822278E-4	0.0
11	0.0	0.0	0.0	3.6817360121644557E-4	0.0
12	0.0	0.0	0.0	5.522604018246683E-4	0.0
13	0.0	0.0	0.0	5.522604018246683E-4	0.0
14	0.0	0.0	0.0	5.522604018246683E-4	0.0
15	0.0	0.0	0.0	5.522604018246683E-4	0.0
16	0.0	0.0	0.0	5.522604018246683E-4	0.0
17	0.0	0.0	0.0	9.204340030411139E-4	0.0
18	0.0	0.0	0.0	9.204340030411139E-4	0.0
19	0.0	0.0	0.0	9.204340030411139E-4	0.0
20	0.0	0.0	0.0	9.204340030411139E-4	0.0
21	0.0	0.0	0.0	0.0011045208036493366	0.0
22	0.0	0.0	0.0	0.0014726944048657823	0.0
23	0.0	0.0	0.0	0.002024954806690451	0.0
24	0.0	0.0	0.0	0.0046021700152055695	0.0
25	0.0	0.0	0.0	0.006074864420071352	0.0
26	0.0	0.0	0.0	0.009020253229802917	0.0
27	0.0	0.0	0.0	0.030742495701573207	0.0
28	1.8408680060822278E-4	0.0	0.0	0.10032730633148142	0.0
29	1.8408680060822278E-4	0.0	0.0	0.2050726958775602	0.0
30	1.8408680060822278E-4	0.0	0.0	0.3447945775392013	0.0
31	1.8408680060822278E-4	0.0	0.0	0.8595012720397922	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	305	0.0	31.540985	1
TAGGACG	25	0.0054938225	29.6	4
TAGACTT	55	1.9002646E-5	23.545454	5
CTTAGAC	45	0.00382325	20.555555	3
GCCGTCT	95	1.6720514E-7	19.473684	36
ATTAGAC	50	0.0070302216	18.5	3
TTAGACT	60	9.22827E-4	18.5	4
CCGTCTT	100	2.8680006E-7	18.5	37
GTTAATG	50	0.0070302216	18.5	3
GTATCAA	535	0.0	17.981308	2
TAGACAG	85	2.7188356E-5	17.411764	5
CTTATAC	510	0.0	17.411764	37
GTGTAGA	85	2.7188356E-5	17.411764	1
TATATCC	100	5.8663154E-6	16.650002	2
CTCTTAT	1485	0.0	15.946128	37
TCTTATA	840	0.0	15.857142	37
GTCTCGC	85	5.357684E-4	15.235294	1
CTTAAGC	75	0.004100702	14.8	3
GTCCTAA	100	1.0916781E-4	14.8	1
CTGACCG	75	0.004100702	14.8	9
>>END_MODULE