FastQCFastQC Report
Fri 10 Feb 2017
ERR1632174.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632174.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3917340
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53410.13634251813730747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44870.11454201065008399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18400.027.0461961
CTTATAC35450.018.9957737
TAGACCG953.6108759E-617.5263165
GTATCAA30250.016.3900832
TCTTATA63400.016.36987337
TATACAC12000.014.95416637
TCGTTTA2950.013.7966130
TCTATAC3550.013.5492963
CGAATTA4400.013.45454515
TACCGAC1254.850206E-513.327
CCTAGTA2254.0745363E-1013.1555562
ATCAACG48350.012.8179942
TATCAAC48450.012.7915371
AATAACG3050.012.7377052
CAACGCA49450.012.7199194
TAGAACA8650.012.6184974
TCAACGC50150.012.5423743
CATTATA4200.011.8928582
CTATACA2808.367351E-1111.8928574
GTACTAG1401.3999554E-411.8928571
Produced by FastQC (version 0.11.5)