##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632174.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3917340
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00615009164382	33.0	31.0	34.0	30.0	34.0
2	32.1791498823181	33.0	31.0	34.0	30.0	34.0
3	32.20661239514568	34.0	31.0	34.0	30.0	34.0
4	35.90997922059356	37.0	35.0	37.0	35.0	37.0
5	35.56403171539871	37.0	35.0	37.0	33.0	37.0
6	35.55591472785104	37.0	35.0	37.0	33.0	37.0
7	35.546031235481216	37.0	35.0	37.0	33.0	37.0
8	35.50425926776843	37.0	35.0	37.0	33.0	37.0
9	37.180286878340915	39.0	37.0	39.0	34.0	39.0
10	36.994316040986995	39.0	37.0	39.0	33.0	39.0
11	37.14757539554902	39.0	37.0	39.0	33.0	39.0
12	37.085991259374985	39.0	37.0	39.0	33.0	39.0
13	37.148318501840535	39.0	37.0	39.0	33.0	39.0
14	38.323624704518885	40.0	38.0	41.0	34.0	41.0
15	38.31106184298529	40.0	38.0	41.0	34.0	41.0
16	38.19547933036193	40.0	38.0	41.0	34.0	41.0
17	38.22678552282927	40.0	38.0	41.0	34.0	41.0
18	38.23595909469181	40.0	38.0	41.0	34.0	41.0
19	38.29414015633057	40.0	38.0	41.0	34.0	41.0
20	38.2643122629131	40.0	38.0	41.0	34.0	41.0
21	38.22931147155978	40.0	38.0	41.0	34.0	41.0
22	38.20585244068679	40.0	38.0	41.0	34.0	41.0
23	38.15595557189317	40.0	38.0	41.0	34.0	41.0
24	38.085739813240615	40.0	38.0	41.0	34.0	41.0
25	38.00163963301628	40.0	37.0	41.0	33.0	41.0
26	37.879695916106336	40.0	37.0	41.0	33.0	41.0
27	37.79061761297207	40.0	37.0	41.0	33.0	41.0
28	37.70374514338812	39.0	37.0	41.0	33.0	41.0
29	37.546378665114595	39.0	37.0	40.0	32.0	41.0
30	37.489916627099	39.0	37.0	40.0	32.0	41.0
31	37.38720866710574	39.0	37.0	40.0	32.0	41.0
32	37.2945950568498	39.0	36.0	40.0	32.0	41.0
33	37.140663051968936	39.0	36.0	40.0	31.0	41.0
34	37.12481428724594	39.0	36.0	40.0	31.0	41.0
35	37.08926005912175	39.0	36.0	40.0	31.0	41.0
36	37.066244441381144	39.0	36.0	40.0	31.0	41.0
37	37.03772611006448	39.0	36.0	40.0	31.0	41.0
38	36.96257970970097	39.0	36.0	40.0	31.0	41.0
39	36.76826673201713	39.0	35.0	40.0	31.0	41.0
40	36.77400634103754	39.0	35.0	40.0	31.0	41.0
41	36.76767679088361	39.0	35.0	40.0	31.0	41.0
42	36.649014382208335	39.0	35.0	40.0	30.0	41.0
43	35.73335732920808	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	3.0
13	3.0
14	7.0
15	27.0
16	37.0
17	90.0
18	176.0
19	361.0
20	736.0
21	1311.0
22	2358.0
23	3827.0
24	6151.0
25	9578.0
26	14237.0
27	20663.0
28	29384.0
29	40419.0
30	55158.0
31	74058.0
32	99082.0
33	134785.0
34	190284.0
35	279504.0
36	436604.0
37	727895.0
38	1138903.0
39	651694.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.47328544369394	18.386379533050487	12.509687696242858	26.630647327012717
2	19.320967799578284	20.425212006106182	33.57684040701088	26.67697978730465
3	20.245983243731715	21.59789551072922	29.72736601877805	28.428755226761016
4	15.62305033517642	15.600892442320555	33.58309465096213	35.19296257154089
5	16.17196873388575	34.76810795080335	33.14920328590319	15.91072002940771
6	35.35935098817055	34.121853094191465	15.376607595970734	15.142188321667255
7	30.27197026553733	29.910015469680957	20.32555254330745	19.492461721474267
8	28.027207237564266	32.64980828827725	19.58139962321371	19.741584850944776
9	27.25106832697698	14.20300510039976	18.903567216529584	39.64235935609368
10	17.526101895674106	26.26379124610067	32.28665369868329	23.923453159541936
11	36.95854840274267	20.773509575375126	21.056431149708732	21.211510872173463
12	22.711687012105152	24.35969305702339	28.182057212292015	24.746562718579444
13	31.398653167710744	18.776057222503027	23.686379022499963	26.13891058728627
14	23.591774009914893	19.692086977387717	24.11427141887097	32.60186759382642
15	26.14802391418667	26.439701430052025	21.35977474510765	26.05249991065366
16	26.163314902459323	25.321519194147047	22.881649282421236	25.633516620972397
17	24.92420877432135	25.438741595061952	24.491312982789342	25.145736647827356
18	25.13131359544997	24.359616474444394	25.221604456084997	25.28746547402064
19	25.84692674110493	24.476455962464325	25.11436331796576	24.56225397846498
20	26.120964736275127	24.183502070282387	24.674268764008232	25.021264429434257
21	26.177508207099713	24.55217060556398	24.05851930136266	25.211801885973646
22	26.404039475766723	24.40183900299693	24.031230375714134	25.162891145522217
23	25.979925153292793	24.31246713330985	24.149065437261	25.558542276136357
24	25.949190011589497	24.196572163764188	24.159250920267326	25.694986904378993
25	25.968105908601245	23.712876594832206	24.405055471314718	25.91396202525183
26	25.55823594582038	24.244410748109686	24.4632071762982	25.734146129771734
27	25.585856729311217	24.336488535587925	24.27210811417952	25.805546620921337
28	25.41461808267855	24.472371558251265	24.64039373656614	25.472616622504045
29	25.227909755088913	24.728540284989304	24.799021785190973	25.244528174730814
30	25.213154844869223	25.003497271107435	24.34361071543445	25.439737168588888
31	25.498476006678	24.541193769241374	24.350758422807314	25.60957180127331
32	25.011385276743916	24.409624898528083	24.77464299754425	25.80434682718375
33	24.997268554682513	24.1903689748656	25.02943323786038	25.782929232591506
34	25.527832661959394	23.685255811341367	24.982641282094484	25.80427024460476
35	25.046281405239267	23.75486937564776	25.55448339944963	25.644365819663346
36	24.798026211664038	23.577708342906156	25.58843500947071	26.035830435959095
37	25.014065667008733	22.40614804944171	26.136357834653108	26.443428448896444
38	24.268789535756405	22.721489582216503	26.89386165101829	26.1158592310088
39	24.072457330739734	22.525872147937122	27.471600626956043	25.930069894367097
40	23.739450749743447	22.223294378328152	28.150122276851132	25.887132595077272
41	23.086226878443025	22.225821603434984	28.701210515298648	25.986741002823344
42	23.067438619062937	22.183420382198126	28.63621743325828	26.112923565480656
43	22.24920481755477	21.91663220450612	29.11056992755288	26.723593050386228
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	150.0
1	173.0
2	196.0
3	495.0
4	794.0
5	794.0
6	1003.0
7	1212.0
8	1304.0
9	1396.0
10	1963.0
11	2530.0
12	2530.0
13	4222.5
14	5915.0
15	9176.0
16	12437.0
17	12783.5
18	13130.0
19	13130.0
20	17025.0
21	20920.0
22	23920.0
23	26920.0
24	33108.0
25	39296.0
26	39296.0
27	44366.0
28	49436.0
29	59794.0
30	70152.0
31	81943.0
32	93734.0
33	93734.0
34	107002.0
35	120270.0
36	136478.5
37	152687.0
38	166819.0
39	180951.0
40	180951.0
41	196334.0
42	211717.0
43	219303.5
44	226890.0
45	237666.5
46	248443.0
47	248443.0
48	256484.5
49	264526.0
50	276823.0
51	289120.0
52	301066.0
53	313012.0
54	313012.0
55	299630.5
56	286249.0
57	277091.5
58	267934.0
59	252090.5
60	236247.0
61	236247.0
62	221429.0
63	206611.0
64	187053.0
65	167495.0
66	147565.0
67	127635.0
68	127635.0
69	111636.0
70	95637.0
71	82197.0
72	68757.0
73	57246.5
74	45736.0
75	45736.0
76	36785.5
77	27835.0
78	23099.5
79	18364.0
80	14676.0
81	10988.0
82	10988.0
83	8514.0
84	6040.0
85	4655.5
86	3271.0
87	2448.0
88	1625.0
89	1625.0
90	1213.5
91	802.0
92	501.0
93	200.0
94	127.5
95	55.0
96	55.0
97	37.5
98	20.0
99	12.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3917340.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.04454980779307
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.29642125204646	49.181996934273705
2	9.588241269019711	11.32266778355543
3	2.8652940320667746	5.0753998857101665
4	1.2927210082243443	3.0531251983073107
5	0.7174427668205929	2.1180542589889684
6	0.44820598221489194	1.58784722646228
7	0.3089978477425409	1.2771247168074724
8	0.23367126323258636	1.1037611632469095
9	0.16505134808833963	0.8770844288740824
>10	0.9289955007834187	10.166635638784143
>50	0.08374186188516604	3.4684663889274367
>100	0.06480594119437025	7.478282065251831
>500	0.005197237880451484	2.0288615439694695
>1k	0.0011693784864257478	1.1241106846950988
>5k	4.33103143120647E-5	0.13658208214560533
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5341	0.13634251813730747	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4487	0.11454201065008399	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.552752633164341E-5	0.0
2	0.0	0.0	0.0	5.105505266328682E-5	0.0
3	0.0	0.0	0.0	7.658257899493023E-5	0.0
4	0.0	0.0	0.0	7.658257899493023E-5	0.0
5	0.0	1.2763763165821705E-4	0.0	7.658257899493023E-5	0.0
6	0.0	1.2763763165821705E-4	0.0	7.658257899493023E-5	0.0
7	0.0	1.2763763165821705E-4	0.0	7.658257899493023E-5	0.0
8	0.0	1.2763763165821705E-4	0.0	7.658257899493023E-5	0.0
9	0.0	1.5316515798986045E-4	0.0	1.2763763165821705E-4	0.0
10	0.0	2.042202106531473E-4	0.0	1.7869268432150389E-4	0.0
11	0.0	2.8080278964807753E-4	0.0	2.042202106531473E-4	0.0
12	0.0	2.8080278964807753E-4	0.0	2.552752633164341E-4	0.0
13	0.0	3.063303159797209E-4	0.0	2.552752633164341E-4	0.0
14	0.0	3.5738536864300777E-4	0.0	2.8080278964807753E-4	0.0
15	0.0	3.5738536864300777E-4	0.0	3.063303159797209E-4	0.0
16	0.0	3.5738536864300777E-4	0.0	3.8291289497465115E-4	0.0
17	0.0	3.5738536864300777E-4	0.0	4.850230003012248E-4	0.0
18	0.0	4.33967947637938E-4	0.0	6.126606319594418E-4	0.0
19	0.0	4.594954739695814E-4	0.0	6.892432109543721E-4	0.0
20	0.0	4.594954739695814E-4	0.0	8.67935895275876E-4	0.0
21	0.0	4.850230003012248E-4	0.0	9.700460006024496E-4	0.0
22	0.0	4.850230003012248E-4	0.0	0.001301903842913814	0.0
23	2.552752633164341E-5	5.871331056277985E-4	0.0	0.001991147053868186	0.0
24	2.552752633164341E-5	5.871331056277985E-4	0.0	0.0034972711074351473	0.0
25	2.552752633164341E-5	5.871331056277985E-4	0.0	0.004952340108338822	0.0
26	2.552752633164341E-5	5.871331056277985E-4	0.0	0.00770931295215631	0.0
27	2.552752633164341E-5	5.871331056277985E-4	0.0	0.030096953545007583	2.552752633164341E-5
28	2.552752633164341E-5	7.913533162809457E-4	0.0	0.10440758269642156	2.552752633164341E-5
29	2.552752633164341E-5	8.168808426125892E-4	0.0	0.21496729923876917	2.552752633164341E-5
30	2.552752633164341E-5	8.168808426125892E-4	0.0	0.3635885575415971	2.552752633164341E-5
31	5.105505266328682E-5	8.168808426125892E-4	0.0	0.8324781612012233	2.552752633164341E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1840	0.0	27.046196	1
CTTATAC	3545	0.0	18.99577	37
TAGACCG	95	3.6108759E-6	17.526316	5
GTATCAA	3025	0.0	16.390083	2
TCTTATA	6340	0.0	16.369873	37
TATACAC	1200	0.0	14.954166	37
TCGTTTA	295	0.0	13.79661	30
TCTATAC	355	0.0	13.549296	3
CGAATTA	440	0.0	13.454545	15
TACCGAC	125	4.850206E-5	13.32	7
CCTAGTA	225	4.0745363E-10	13.155556	2
ATCAACG	4835	0.0	12.817994	2
TATCAAC	4845	0.0	12.791537	1
AATAACG	305	0.0	12.737705	2
CAACGCA	4945	0.0	12.719919	4
TAGAACA	865	0.0	12.618497	4
TCAACGC	5015	0.0	12.542374	3
CATTATA	420	0.0	11.892858	2
CTATACA	280	8.367351E-11	11.892857	4
GTACTAG	140	1.3999554E-4	11.892857	1
>>END_MODULE