FastQCFastQC Report
Fri 10 Feb 2017
ERR1632173.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632173.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498838
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49991.0021289476743953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39720.7962504861297656No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35220.706040838909626No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12680.2541907392780823No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT6510.13050328964513527TruSeq Adapter, Index 6 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12950.031.8571431
GGCTATA250.005493398329.630
ACTCTTA451.3217458E-424.66666614
CCCATAG851.9699655E-923.9411771
AAGGCGT400.001929663523.12523
TAGACAG502.699132E-422.25
CCCTCGA450.003822818220.55555526
CGTCTTC450.003822818220.55555537
AAGTTTC450.003822818220.55555519
GCTCGAA450.003822818220.55555532
TCGAATA450.003822818220.55555534
CTCGATG656.892123E-519.92307728
GGCCTAA951.6713602E-719.47368410
ATTAGAG1103.8386133E-818.53
CAACGGT500.00702943718.58
CTCGAAT500.00702943718.533
AATAGAC500.00702943718.52
GCCGTCT1409.276846E-1118.536
CTAATTA500.00702943718.528
GTAAACA500.00702943718.521