##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632173.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	498838
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.713295699204956	34.0	31.0	34.0	31.0	34.0
2	32.86113126906932	34.0	31.0	34.0	31.0	34.0
3	32.850137720061426	34.0	31.0	34.0	31.0	34.0
4	36.323048765330626	37.0	37.0	37.0	35.0	37.0
5	36.26172224249155	37.0	35.0	37.0	35.0	37.0
6	36.32614395855969	37.0	37.0	37.0	35.0	37.0
7	36.318065183486425	37.0	37.0	37.0	35.0	37.0
8	36.310882490908874	37.0	37.0	37.0	35.0	37.0
9	38.110641129986085	39.0	39.0	39.0	37.0	39.0
10	38.033108945188616	39.0	38.0	39.0	35.0	39.0
11	38.14511324317714	39.0	38.0	39.0	37.0	39.0
12	38.091003492115675	39.0	38.0	39.0	37.0	39.0
13	38.13656738259716	39.0	39.0	39.0	37.0	39.0
14	39.4444829784419	40.0	39.0	41.0	37.0	41.0
15	39.46963342808687	40.0	39.0	41.0	37.0	41.0
16	39.365788091524706	40.0	39.0	41.0	37.0	41.0
17	39.452120728573206	40.0	39.0	41.0	37.0	41.0
18	39.51050441225408	40.0	39.0	41.0	37.0	41.0
19	39.592847778236624	41.0	39.0	41.0	37.0	41.0
20	39.57899959505892	41.0	39.0	41.0	37.0	41.0
21	39.55791659817416	41.0	39.0	41.0	37.0	41.0
22	39.53319314085936	41.0	39.0	41.0	37.0	41.0
23	39.49353297062373	41.0	39.0	41.0	37.0	41.0
24	39.427014381422424	41.0	39.0	41.0	37.0	41.0
25	39.36342058944988	40.0	39.0	41.0	36.0	41.0
26	39.25893777138069	40.0	39.0	41.0	36.0	41.0
27	39.163975078081464	40.0	39.0	41.0	36.0	41.0
28	39.13485339929997	40.0	39.0	41.0	35.0	41.0
29	38.99756834884271	40.0	39.0	41.0	35.0	41.0
30	38.902886307779276	40.0	38.0	41.0	35.0	41.0
31	38.77114012966133	40.0	38.0	41.0	35.0	41.0
32	38.66380267742233	40.0	38.0	41.0	35.0	41.0
33	38.51350538651827	40.0	38.0	41.0	35.0	41.0
34	38.518579178009695	40.0	38.0	41.0	35.0	41.0
35	38.44933024348586	40.0	38.0	41.0	35.0	41.0
36	38.40882009790754	40.0	38.0	41.0	35.0	41.0
37	38.359178731371706	40.0	38.0	41.0	35.0	41.0
38	38.279667948311875	40.0	38.0	41.0	35.0	41.0
39	38.103111631431446	40.0	38.0	41.0	34.0	41.0
40	38.04448538403249	40.0	38.0	41.0	34.0	41.0
41	37.99213171410358	40.0	38.0	41.0	34.0	41.0
42	37.82589137154748	40.0	37.0	41.0	34.0	41.0
43	37.02708895473079	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	1.0
15	3.0
16	1.0
17	4.0
18	5.0
19	8.0
20	24.0
21	47.0
22	85.0
23	174.0
24	278.0
25	443.0
26	686.0
27	972.0
28	1574.0
29	2234.0
30	3219.0
31	4395.0
32	5842.0
33	8121.0
34	12284.0
35	18244.0
36	29295.0
37	62434.0
38	127212.0
39	221250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.55061162140815	19.11241725770691	14.072304034576355	23.26466708630858
2	19.578500435010966	21.39592412767271	34.841571812893164	24.18400362442316
3	19.964998656878585	23.70990181181065	30.582674134689018	25.742425396621748
4	13.85580088124802	16.352603450418773	35.62118363075788	34.170412037575325
5	13.862015323612075	36.586426856013375	33.96313031485171	15.588427505522834
6	32.10801903624022	36.11753715635136	15.558758554881544	16.215685252526875
7	27.53659504688897	30.996034784839964	21.06695961414327	20.40041055412779
8	28.60287307703102	31.869264169930922	19.62861690568882	19.89924584734924
9	27.02520658009213	12.491229617631374	20.286545932747703	40.197017869528786
10	18.788263925362543	26.777029817295393	31.995758141921826	22.43894811542024
11	35.00755756377822	21.875438519118433	19.912476595608194	23.204527321495156
12	22.06006759709565	29.37767371371066	27.036031737758552	21.526226951435135
13	33.0081108496145	19.53239328198734	23.47154787726677	23.98794799113139
14	24.324730674086577	20.73959882767552	26.389529266014218	28.546141232223686
15	26.336004875330264	26.47492773204928	22.759493061875798	24.42957433074465
16	24.625429498153707	24.647681211134678	24.856366195037268	25.870523095674347
17	21.35663281466127	27.48587717856298	25.630364968186065	25.52712503858968
18	22.398253541229817	24.503546241465166	27.327910062986383	25.770290154318637
19	24.220688880959347	24.370837827110204	28.83541350097627	22.57305979095418
20	25.974765354684287	24.042875642994318	28.287540243525953	21.69481875879544
21	26.676195478291547	23.821962240246332	25.588668064582087	23.91317421688003
22	26.268848804621943	23.78407418841387	27.355774820683266	22.591302186280917
23	24.920715743387632	24.406119822467414	26.58618629695412	24.086978137190833
24	22.734434826536873	25.49184304323247	27.519354980975784	24.25436714925487
25	24.255169012785714	25.06745676953239	27.383238646614732	23.29413557106716
26	23.52807925619139	25.016738901206402	27.165131766224704	24.2900500763775
27	22.675297391137004	25.95973041348093	27.32029235944335	24.04467983593872
28	22.943721208087595	24.97263640700989	28.386770855468107	23.696871529434404
29	22.72300827122232	25.124990477870572	28.802737562094304	23.3492636888128
30	23.37131493591106	26.35204214594718	26.805696438523128	23.470946479618636
31	24.08798046660439	26.15718930795168	26.867840862163668	22.886989363280264
32	22.503297663770603	25.9960147382517	27.96960135354564	23.53108624443206
33	22.04743824648483	25.799157241429082	28.29415561765543	23.859248894430657
34	23.4142146348113	23.88350526623874	28.82318508213087	23.87909501681909
35	22.239885493887794	24.967023362293972	29.0851939908347	23.707897152983534
36	22.612150638082902	25.388202181870668	27.46743431735353	24.532212862692898
37	21.864412895569302	24.173378932639455	29.15956683332064	24.802641338470604
38	21.62124777984035	24.584935389846002	29.457058203264385	24.336758627049264
39	21.235350955620863	24.0601157089075	30.4648001956547	24.239733139816934
40	20.75142631475549	23.015487994098287	32.40972018972091	23.82336550142531
41	20.344079641085884	22.938108163371677	31.73996367558205	24.97784851996039
42	19.31669199218985	23.490592136124352	32.6482745901475	24.544441281538294
43	19.181978919007776	22.830257518472933	32.632638251296015	25.355125311223283
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	49.0
1	55.0
2	61.0
3	224.0
4	387.0
5	387.0
6	496.0
7	605.0
8	633.5
9	662.0
10	995.0
11	1328.0
12	1328.0
13	2457.5
14	3587.0
15	6011.0
16	8435.0
17	7428.0
18	6421.0
19	6421.0
20	6586.0
21	6751.0
22	4779.0
23	2807.0
24	2723.5
25	2640.0
26	2640.0
27	3171.5
28	3703.0
29	4791.5
30	5880.0
31	7313.0
32	8746.0
33	8746.0
34	11208.0
35	13670.0
36	15912.0
37	18154.0
38	20879.5
39	23605.0
40	23605.0
41	27184.5
42	30764.0
43	34187.0
44	37610.0
45	40281.5
46	42953.0
47	42953.0
48	43891.0
49	44829.0
50	44928.0
51	45027.0
52	43673.5
53	42320.0
54	42320.0
55	41691.5
56	41063.0
57	37110.5
58	33158.0
59	30159.5
60	27161.0
61	27161.0
62	23124.0
63	19087.0
64	14895.5
65	10704.0
66	8443.5
67	6183.0
68	6183.0
69	4674.5
70	3166.0
71	2609.5
72	2053.0
73	1803.5
74	1554.0
75	1554.0
76	1276.0
77	998.0
78	996.0
79	994.0
80	882.5
81	771.0
82	771.0
83	606.5
84	442.0
85	316.5
86	191.0
87	168.5
88	146.0
89	146.0
90	133.5
91	121.0
92	82.0
93	43.0
94	24.5
95	6.0
96	6.0
97	4.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	498838.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.64739077686096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.78866064022147	46.023378015894714
2	7.0366902575578445	7.550001440458722
3	2.0867721746082153	3.358496469404605
4	1.034935269926544	2.220863074180215
5	0.6727427418404835	1.8045446381906656
6	0.4445918719204159	1.4310716333478402
7	0.35374684383724325	1.328431661719246
8	0.29244689751458386	1.2551210393956391
9	0.2505538696421534	1.2097405219812558
>10	1.7861090533867516	19.214698122114847
>50	0.1934122518189535	7.185070803003976
>100	0.05746033887328922	4.514896853483808
>500	3.75557770413655E-4	0.13116149099102856
>1k	0.00150223108165462	2.772524235833401
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4999	1.0021289476743953	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3972	0.7962504861297656	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3522	0.706040838909626	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1268	0.2541907392780823	No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	651	0.13050328964513527	TruSeq Adapter, Index 6 (95% over 21bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.0046588271142133E-4	0.0
2	0.0	0.0	0.0	2.0046588271142133E-4	0.0
3	0.0	0.0	0.0	2.0046588271142133E-4	0.0
4	0.0	0.0	0.0	2.0046588271142133E-4	0.0
5	0.0	2.0046588271142133E-4	0.0	2.0046588271142133E-4	0.0
6	0.0	2.0046588271142133E-4	0.0	2.0046588271142133E-4	0.0
7	0.0	2.0046588271142133E-4	0.0	2.0046588271142133E-4	0.0
8	0.0	2.0046588271142133E-4	0.0	2.0046588271142133E-4	0.0
9	0.0	2.0046588271142133E-4	0.0	4.0093176542284266E-4	0.0
10	0.0	2.0046588271142133E-4	0.0	4.0093176542284266E-4	0.0
11	0.0	2.0046588271142133E-4	0.0	4.0093176542284266E-4	0.0
12	0.0	2.0046588271142133E-4	0.0	6.013976481342641E-4	0.0
13	0.0	2.0046588271142133E-4	0.0	8.018635308456853E-4	0.0
14	0.0	2.0046588271142133E-4	0.0	8.018635308456853E-4	0.0
15	0.0	2.0046588271142133E-4	0.0	8.018635308456853E-4	0.0
16	0.0	2.0046588271142133E-4	0.0	0.0020046588271142134	0.0
17	0.0	2.0046588271142133E-4	0.0	0.0032074541233827413	0.0
18	0.0	2.0046588271142133E-4	0.0	0.003407920006094163	0.0
19	0.0	2.0046588271142133E-4	0.0	0.004009317654228427	0.0
20	0.0	2.0046588271142133E-4	0.0	0.005011647067785533	0.0
21	0.0	2.0046588271142133E-4	0.0	0.006815840012188326	0.0
22	0.0	2.0046588271142133E-4	0.0	0.010624691783705332	0.0
23	0.0	2.0046588271142133E-4	0.0	0.013030282376242387	0.0
24	0.0	2.0046588271142133E-4	0.0	0.0206479859192764	0.0
25	0.0	2.0046588271142133E-4	0.0	0.024657303573504825	0.0
26	0.0	2.0046588271142133E-4	0.0	0.02986941652400178	0.0
27	0.0	2.0046588271142133E-4	0.0	0.051920663622258126	0.0
28	0.0	2.0046588271142133E-4	0.0	0.11787393903431576	0.0
29	0.0	2.0046588271142133E-4	0.0	0.26341216988280763	0.0
30	0.0	2.0046588271142133E-4	0.0	0.4903395491121366	0.0
31	0.0	2.0046588271142133E-4	0.0	1.0813129713454068	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1295	0.0	31.857143	1
GGCTATA	25	0.0054933983	29.6	30
ACTCTTA	45	1.3217458E-4	24.666666	14
CCCATAG	85	1.9699655E-9	23.941177	1
AAGGCGT	40	0.0019296635	23.125	23
TAGACAG	50	2.699132E-4	22.2	5
CCCTCGA	45	0.0038228182	20.555555	26
CGTCTTC	45	0.0038228182	20.555555	37
AAGTTTC	45	0.0038228182	20.555555	19
GCTCGAA	45	0.0038228182	20.555555	32
TCGAATA	45	0.0038228182	20.555555	34
CTCGATG	65	6.892123E-5	19.923077	28
GGCCTAA	95	1.6713602E-7	19.473684	10
ATTAGAG	110	3.8386133E-8	18.5	3
CAACGGT	50	0.007029437	18.5	8
CTCGAAT	50	0.007029437	18.5	33
AATAGAC	50	0.007029437	18.5	2
GCCGTCT	140	9.276846E-11	18.5	36
CTAATTA	50	0.007029437	18.5	28
GTAAACA	50	0.007029437	18.5	21
>>END_MODULE