FastQCFastQC Report
Fri 10 Feb 2017
ERR1632172.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632172.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1099962
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36790.3344660997379909No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28920.262918173536904No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25430.23118980473870915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9500.030.184211
TTAGACG609.2364696E-418.54
TAGATTC500.007034695218.55
GTATCAA15950.017.9780562
ATAATAC1054.7968024E-717.6190473
CGTATTA752.0671648E-417.26666615
CGAATTA752.0671648E-417.26666615
GTTCTAA650.001579875517.0769231
GCGCAAG1757.2759576E-1216.9142861
GCTATAC700.002592399515.8571423
ACTCGCA700.002592399515.85714221
TATACTG700.002592399515.8571425
TAAGACT1302.5884583E-715.6538464
TTATGCT1802.0190782E-1015.4166664
ACAGCGT855.363891E-415.2352938
TATTAAC855.363891E-415.2352932
GTGTTAG1651.6443664E-814.5757591
TTTAGAC1152.2095626E-514.4782613
ACAGTAC908.273615E-414.3888883
TTACACT1304.4432527E-614.2307694
Produced by FastQC (version 0.11.5)