##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632172.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1099962
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.341129966307925	33.0	31.0	34.0	31.0	34.0
2	32.50449470072603	34.0	31.0	34.0	31.0	34.0
3	32.51184313639926	34.0	31.0	34.0	31.0	34.0
4	36.10747098536131	37.0	35.0	37.0	35.0	37.0
5	35.93025395422751	37.0	35.0	37.0	35.0	37.0
6	35.95167378509439	37.0	35.0	37.0	35.0	37.0
7	35.94163162000142	37.0	35.0	37.0	35.0	37.0
8	35.92057271069364	37.0	35.0	37.0	35.0	37.0
9	37.63080542782387	39.0	37.0	39.0	35.0	39.0
10	37.51040308665208	39.0	37.0	39.0	35.0	39.0
11	37.63683018140627	39.0	37.0	39.0	35.0	39.0
12	37.586385711506395	39.0	37.0	39.0	35.0	39.0
13	37.624483391244425	39.0	37.0	39.0	35.0	39.0
14	38.896181868100896	40.0	38.0	41.0	36.0	41.0
15	38.907826815835456	40.0	38.0	41.0	36.0	41.0
16	38.79554566430477	40.0	38.0	41.0	36.0	41.0
17	38.84270911176932	40.0	38.0	41.0	36.0	41.0
18	38.858316923675545	40.0	38.0	41.0	36.0	41.0
19	38.91618619552312	40.0	38.0	41.0	36.0	41.0
20	38.88615243072034	40.0	38.0	41.0	35.0	41.0
21	38.855691378429434	40.0	38.0	41.0	35.0	41.0
22	38.82847316543663	40.0	38.0	41.0	35.0	41.0
23	38.79171098637953	40.0	38.0	41.0	35.0	41.0
24	38.7304025048138	40.0	38.0	41.0	35.0	41.0
25	38.65259527147301	40.0	38.0	41.0	35.0	41.0
26	38.5451961067746	40.0	38.0	41.0	35.0	41.0
27	38.45847765650086	40.0	38.0	41.0	34.0	41.0
28	38.386496988077766	40.0	38.0	41.0	34.0	41.0
29	38.24002010978561	40.0	38.0	41.0	34.0	41.0
30	38.17354235873603	40.0	38.0	41.0	34.0	41.0
31	38.07382891408976	40.0	38.0	41.0	34.0	41.0
32	37.985354039503186	40.0	37.0	41.0	33.0	41.0
33	37.829263192728476	40.0	37.0	41.0	33.0	41.0
34	37.8140308483384	40.0	37.0	41.0	33.0	41.0
35	37.77395128195338	40.0	37.0	41.0	33.0	41.0
36	37.73342806387857	40.0	37.0	41.0	33.0	41.0
37	37.69596949712808	40.0	37.0	41.0	33.0	41.0
38	37.618014985972245	40.0	37.0	41.0	33.0	41.0
39	37.41777897781923	40.0	37.0	41.0	32.0	41.0
40	37.41234788110862	40.0	36.0	41.0	32.0	41.0
41	37.39417270778445	39.0	36.0	41.0	32.0	41.0
42	37.25895712760986	39.0	36.0	41.0	32.0	41.0
43	36.38925708342652	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	0.0
14	1.0
15	3.0
16	7.0
17	14.0
18	31.0
19	71.0
20	128.0
21	244.0
22	393.0
23	721.0
24	1290.0
25	1908.0
26	2817.0
27	4180.0
28	6184.0
29	8337.0
30	11652.0
31	15618.0
32	20569.0
33	27588.0
34	39415.0
35	57323.0
36	90270.0
37	161542.0
38	329417.0
39	320237.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.95539300448561	18.280176951567416	12.377973057251069	26.386456986695904
2	19.467581607364618	20.43334224273202	33.25342148183301	26.845654668070352
3	20.470070784263456	21.724568666917584	29.705026173631456	28.100334375187508
4	15.439078804540522	15.514808693391227	33.40960869557312	35.636503806495135
5	16.017371509197588	34.87175011500398	33.101688967437056	16.00918940836138
6	35.151759788065405	34.07372254677889	15.243981155712651	15.530536509443055
7	30.15467807069699	29.631296353874042	20.283882534123908	19.93014304130506
8	28.59071495197107	31.918739010984016	19.290484580376415	20.200061456668504
9	27.24721399466527	13.579650933395882	18.918380816791853	40.254754255147
10	17.925437424201927	25.90789500000909	32.13338278958728	24.033284786201705
11	36.918729919760864	20.829537747667647	20.563528558259286	21.688203774312203
12	22.69505673832369	24.922860971560837	27.680319865595354	24.701762424520123
13	32.00610566546844	18.834377914873425	23.26207632627309	25.897440093385043
14	23.744547538914983	19.726045081557363	24.17038043132399	32.35902694820367
15	26.515279618750466	26.33200056001935	21.255006991150605	25.897712830079584
16	26.03817222776787	24.844040066838673	23.086070246063045	26.031717459330412
17	24.66257925273782	25.39778646898711	24.527301852245806	25.41233242602926
18	24.710944559902977	24.137288379053096	25.46151594327804	25.69025111776589
19	25.654704435244124	24.32102199894178	25.689523819913777	24.334749745900314
20	25.837710757280707	24.000647295088378	25.31541998723592	24.846221960394995
21	26.28336251615965	24.223927735685415	24.387206103483575	25.105503644671362
22	26.57109972889973	23.96146412330608	24.438116953131107	25.029319194663092
23	26.03553577305398	24.064104032684767	24.43157127246214	25.468788921799117
24	25.616703122471502	24.139924833766983	24.415661631947287	25.827710411814227
25	25.686250979579295	23.75082048288941	24.899132879135824	25.663795658395472
26	25.24314476318273	24.150197915928004	24.860767917437148	25.74588940345212
27	25.192961211387306	24.254110596547882	24.85294946552699	25.699978726537825
28	25.121776934112273	24.27965693360316	25.27287306288763	25.325693069396944
29	25.03441027962784	24.488845978315616	25.2557815633631	25.220962178693444
30	24.897587371200096	24.9300430378504	24.863858933308606	25.3085106576409
31	25.32732948956419	24.541484160361904	24.843312768986564	25.287873581087343
32	24.676670648622405	24.616668575823528	25.200325102139892	25.50633567341417
33	24.69776228633353	24.45166287562661	25.31742005632922	25.533154781710643
34	25.147596007862088	24.02864826239452	25.406604955443918	25.417150774299476
35	24.80922068216902	24.336386166067555	25.694069431489453	25.160323720273976
36	24.84849476618283	24.40729770664805	25.16232378936727	25.581883737801853
37	24.720581256443406	23.332987866853582	25.881712277333218	26.064718599369797
38	24.18292631927285	23.854187690120206	26.490915140704864	25.471970849902085
39	23.850823937554207	23.559541147785104	27.046570699715083	25.54306421494561
40	23.691727532405665	23.05115994916188	27.826143084942935	25.43096943348952
41	23.223529540111386	22.861971595382386	28.23233893534504	25.68215992916119
42	23.01924975590066	22.725603248112208	28.373707455348455	25.881439540638677
43	22.501686421894572	22.147765104612706	28.867997258087097	26.482551215405625
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	31.5
2	46.0
3	137.0
4	228.0
5	228.0
6	338.5
7	449.0
8	472.5
9	496.0
10	733.5
11	971.0
12	971.0
13	1786.0
14	2601.0
15	4681.0
16	6761.0
17	6471.5
18	6182.0
19	6182.0
20	7042.0
21	7902.0
22	7620.0
23	7338.0
24	8713.5
25	10089.0
26	10089.0
27	11725.0
28	13361.0
29	16414.0
30	19467.0
31	22588.5
32	25710.0
33	25710.0
34	30021.5
35	34333.0
36	38679.5
37	43026.0
38	46691.5
39	50357.0
40	50357.0
41	54779.5
42	59202.0
43	61629.0
44	64056.0
45	67794.5
46	71533.0
47	71533.0
48	73026.5
49	74520.0
50	76558.0
51	78596.0
52	80399.5
53	82203.0
54	82203.0
55	80246.0
56	78289.0
57	75273.0
58	72257.0
59	68419.0
60	64581.0
61	64581.0
62	60818.0
63	57055.0
64	51467.5
65	45880.0
66	40954.0
67	36028.0
68	36028.0
69	31555.5
70	27083.0
71	23622.0
72	20161.0
73	17638.0
74	15115.0
75	15115.0
76	12624.5
77	10134.0
78	8104.0
79	6074.0
80	4830.0
81	3586.0
82	3586.0
83	2849.0
84	2112.0
85	1636.0
86	1160.0
87	891.5
88	623.0
89	623.0
90	442.0
91	261.0
92	172.5
93	84.0
94	50.5
95	17.0
96	17.0
97	15.0
98	13.0
99	9.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1099962.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.4253070775326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.1065504135183	59.60292460509563
2	8.113063335938559	11.102777002021336
3	1.9091958912909022	3.9191194539823053
4	0.807052733585152	2.2089132449330444
5	0.4355720210931803	1.4902074648841184
6	0.28891021578733245	1.1861262139850655
7	0.19234262269036256	0.9212772115180232
8	0.14218231172426107	0.778309467258082
9	0.11523824378394719	0.7096690996192933
>10	0.7829155690215901	10.748958624111662
>50	0.08101668864162684	3.754307599468599
>100	0.02542744197011985	2.6668437456002456
>500	1.3312773865178264E-4	0.08034408242847016
>1k	3.9938321595534796E-4	0.8302221850941917
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3679	0.3344660997379909	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2892	0.262918173536904	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2543	0.23118980473870915	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.8182446302690458E-4	9.091223151345229E-5	0.0	0.0	0.0
3	2.7273669454035685E-4	1.8182446302690458E-4	0.0	0.0	0.0
4	2.7273669454035685E-4	1.8182446302690458E-4	0.0	9.091223151345229E-5	0.0
5	4.545611575672614E-4	3.6364892605380916E-4	0.0	9.091223151345229E-5	0.0
6	4.545611575672614E-4	3.6364892605380916E-4	0.0	9.091223151345229E-5	0.0
7	4.545611575672614E-4	3.6364892605380916E-4	0.0	1.8182446302690458E-4	0.0
8	4.545611575672614E-4	3.6364892605380916E-4	0.0	1.8182446302690458E-4	0.0
9	4.545611575672614E-4	3.6364892605380916E-4	0.0	1.8182446302690458E-4	0.0
10	5.454733890807137E-4	3.6364892605380916E-4	0.0	2.7273669454035685E-4	0.0
11	5.454733890807137E-4	3.6364892605380916E-4	0.0	2.7273669454035685E-4	0.0
12	5.454733890807137E-4	3.6364892605380916E-4	0.0	2.7273669454035685E-4	9.091223151345229E-5
13	7.272978521076183E-4	4.545611575672614E-4	0.0	2.7273669454035685E-4	9.091223151345229E-5
14	7.272978521076183E-4	5.454733890807137E-4	0.0	3.6364892605380916E-4	9.091223151345229E-5
15	9.091223151345228E-4	5.454733890807137E-4	0.0	4.545611575672614E-4	9.091223151345229E-5
16	9.091223151345228E-4	5.454733890807137E-4	0.0	0.001000034546647975	1.8182446302690458E-4
17	9.091223151345228E-4	5.454733890807137E-4	0.0	0.001272771241188332	1.8182446302690458E-4
18	9.091223151345228E-4	5.454733890807137E-4	0.0	0.001272771241188332	1.8182446302690458E-4
19	0.0011818590096748798	5.454733890807137E-4	0.0	0.0013636834727017843	1.8182446302690458E-4
20	0.0011818590096748798	5.454733890807137E-4	0.0	0.0016364201672421411	1.8182446302690458E-4
21	0.0011818590096748798	5.454733890807137E-4	0.0	0.00200006909329595	1.8182446302690458E-4
22	0.0011818590096748798	5.454733890807137E-4	0.0	0.002272805787836307	1.8182446302690458E-4
23	0.0011818590096748798	6.36385620594166E-4	0.0	0.00318192810297083	1.8182446302690458E-4
24	0.0011818590096748798	7.272978521076183E-4	0.0	0.00436378711264571	1.8182446302690458E-4
25	0.0011818590096748798	7.272978521076183E-4	0.0	0.005363821659293684	1.8182446302690458E-4
26	0.0011818590096748798	7.272978521076183E-4	0.0	0.008454837530751063	1.8182446302690458E-4
27	0.0011818590096748798	7.272978521076183E-4	0.0	0.02500086366619938	1.8182446302690458E-4
28	0.0011818590096748798	7.272978521076183E-4	0.0	0.07854816802762277	1.8182446302690458E-4
29	0.001272771241188332	8.182100836210706E-4	0.0	0.1530052856371402	1.8182446302690458E-4
30	0.0013636834727017843	8.182100836210706E-4	0.0	0.24528120062329425	1.8182446302690458E-4
31	0.0013636834727017843	8.182100836210706E-4	0.0	0.5038355870475526	1.8182446302690458E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	950	0.0	30.18421	1
TTAGACG	60	9.2364696E-4	18.5	4
TAGATTC	50	0.0070346952	18.5	5
GTATCAA	1595	0.0	17.978056	2
ATAATAC	105	4.7968024E-7	17.619047	3
CGTATTA	75	2.0671648E-4	17.266666	15
CGAATTA	75	2.0671648E-4	17.266666	15
GTTCTAA	65	0.0015798755	17.076923	1
GCGCAAG	175	7.2759576E-12	16.914286	1
GCTATAC	70	0.0025923995	15.857142	3
ACTCGCA	70	0.0025923995	15.857142	21
TATACTG	70	0.0025923995	15.857142	5
TAAGACT	130	2.5884583E-7	15.653846	4
TTATGCT	180	2.0190782E-10	15.416666	4
ACAGCGT	85	5.363891E-4	15.235293	8
TATTAAC	85	5.363891E-4	15.235293	2
GTGTTAG	165	1.6443664E-8	14.575759	1
TTTAGAC	115	2.2095626E-5	14.478261	3
ACAGTAC	90	8.273615E-4	14.388888	3
TTACACT	130	4.4432527E-6	14.230769	4
>>END_MODULE