FastQCFastQC Report
Fri 10 Feb 2017
ERR1632171.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632171.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12607
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT960.7614817165067026TruSeq Adapter, Index 3 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT480.3807408582533513No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC380.3014198461172365No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC360.2855556436900135No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC360.2855556436900135RNA PCR Primer, Index 3 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG220.17450622669945268No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG130.10311731577694933No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTTA400.001827788323.12537
GTCTCTT500.006665176718.536
Produced by FastQC (version 0.11.5)