FastQCFastQC Report
Fri 10 Feb 2017
ERR1632170.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632170.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011652
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40960.20361374631397477No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28970.14401099196083617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28850.14341446731343194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG303.60043E-430.8333345
GGTATCA16650.024.01
CTTATAC25950.018.107937
CGTATAC650.001580491117.0769233
CGAATTA1107.8147605E-716.81818215
GTACTAT2200.015.9772731
CCGTATA700.002593400415.8571422
TATACAC9000.015.82777837
TCTTATA43600.015.52981737
TTACTCG1201.9371582E-615.41666719
TATGCCG1601.0977601E-815.0312533
TCTATCG1001.0940115E-414.831
GGCTACG1508.110146E-814.81
CGAACGA1252.9612347E-614.79999916
GTATCAA27150.014.5819522
CGTATGC2059.276846E-1114.43902431
ATGCGTT1803.3342076E-914.38888936
GTCCTAT2353.6379788E-1214.1702121
TACGACG1702.4394467E-814.14705855
GCTACGA1702.4394467E-814.14705852
Produced by FastQC (version 0.11.5)