##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632170.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2011652
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74999652027289	34.0	31.0	34.0	31.0	34.0
2	32.904929878527696	34.0	31.0	34.0	31.0	34.0
3	32.917899815673884	34.0	33.0	34.0	31.0	34.0
4	36.36992084117929	37.0	37.0	37.0	35.0	37.0
5	36.28259609514966	37.0	37.0	37.0	35.0	37.0
6	36.317952111001304	37.0	37.0	37.0	35.0	37.0
7	36.30820141853561	37.0	37.0	37.0	35.0	37.0
8	36.290271876050134	37.0	37.0	37.0	35.0	37.0
9	38.075663683380625	39.0	39.0	39.0	37.0	39.0
10	38.005697307486585	39.0	38.0	39.0	35.0	39.0
11	38.115605979563064	39.0	38.0	39.0	37.0	39.0
12	38.08353979714185	39.0	38.0	39.0	37.0	39.0
13	38.11495924742451	39.0	38.0	39.0	37.0	39.0
14	39.4987134951771	40.0	39.0	41.0	37.0	41.0
15	39.52183976154922	40.0	39.0	41.0	37.0	41.0
16	39.407657487477955	40.0	39.0	41.0	37.0	41.0
17	39.46398035047812	40.0	39.0	41.0	37.0	41.0
18	39.48771805461382	41.0	39.0	41.0	37.0	41.0
19	39.543649199762186	41.0	39.0	41.0	37.0	41.0
20	39.527317349124004	41.0	39.0	41.0	37.0	41.0
21	39.499198171453116	41.0	39.0	41.0	37.0	41.0
22	39.476083338470076	41.0	39.0	41.0	37.0	41.0
23	39.44042408925599	41.0	39.0	41.0	37.0	41.0
24	39.38955445574086	41.0	39.0	41.0	37.0	41.0
25	39.32603551707751	40.0	39.0	41.0	36.0	41.0
26	39.25281808185511	40.0	39.0	41.0	36.0	41.0
27	39.181510022608286	40.0	39.0	41.0	36.0	41.0
28	39.11554632709832	40.0	39.0	41.0	36.0	41.0
29	38.97740613187569	40.0	39.0	41.0	35.0	41.0
30	38.93609182900422	40.0	39.0	41.0	35.0	41.0
31	38.85891595564243	40.0	38.0	41.0	35.0	41.0
32	38.80248770662122	40.0	38.0	41.0	35.0	41.0
33	38.65729360744304	40.0	38.0	41.0	35.0	41.0
34	38.67087398814507	40.0	38.0	41.0	35.0	41.0
35	38.64106167468329	40.0	38.0	41.0	35.0	41.0
36	38.61439304611334	40.0	38.0	41.0	35.0	41.0
37	38.59095857534007	40.0	38.0	41.0	35.0	41.0
38	38.52842439944881	40.0	38.0	41.0	35.0	41.0
39	38.35264349897497	40.0	38.0	41.0	34.0	41.0
40	38.352197099697165	40.0	38.0	41.0	34.0	41.0
41	38.36077263860747	40.0	38.0	41.0	34.0	41.0
42	38.22851417640825	40.0	38.0	41.0	34.0	41.0
43	37.39020963864525	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	3.0
12	3.0
13	0.0
14	2.0
15	4.0
16	7.0
17	11.0
18	28.0
19	49.0
20	97.0
21	202.0
22	429.0
23	683.0
24	1132.0
25	1760.0
26	2661.0
27	4053.0
28	5905.0
29	8423.0
30	11897.0
31	16433.0
32	22712.0
33	32131.0
34	47134.0
35	72021.0
36	115802.0
37	216124.0
38	508281.0
39	943663.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.24202197994484	17.686707243598793	12.398913927458626	26.67235684899774
2	19.288972446526536	20.166708754794566	33.13152573109067	27.412793067588233
3	19.963940085064415	21.148638034809203	30.007029048761915	28.88039283136447
4	14.823587777607658	15.21371489701002	34.48111303545544	35.481584289926886
5	15.862684002998531	34.69362494109319	33.78427282651273	15.659418229395541
6	35.554260876135636	34.33088824508414	15.220525220067884	14.894325658712344
7	30.34903651327367	29.795411930095266	20.344721651657444	19.510829904973622
8	28.914593577815644	31.235869822414614	19.766987530646453	20.08254906912329
9	27.591849882584064	13.721309649979222	18.835713135273892	39.85112733216282
10	17.735970237396927	25.73223400468868	32.14502309544593	24.38677266246846
11	37.169898173242686	20.699653816862956	20.578211340728913	21.552236669165442
12	23.680437769554572	24.667437509072148	27.510374557826108	24.141750163547172
13	32.43627625454105	18.380465408529904	23.097036664393244	26.08622167253581
14	23.784879293237598	19.6087096575352	24.04536172260411	32.56104932662309
15	26.52784875316407	26.362859977769514	21.20381656469409	25.905474704372327
16	25.922028263337793	25.136504723481	22.75463151678322	26.186835496397986
17	25.014565143474123	24.93527707575664	24.300972534016818	25.749185246752422
18	25.06571713198903	23.60229304074462	25.533392455553944	25.798597371712408
19	26.183753452386398	23.925659110024995	25.12974411081042	24.76084332677819
20	26.953667930636115	23.615665134923933	24.156066755084876	25.274600179355076
21	27.132277352146396	23.750280863688154	23.820074247434448	25.297367536731002
22	27.276536896043652	23.971442376713266	23.81475523599509	24.937265491247988
23	26.557128171274158	23.64325439986638	23.750280863688154	26.04933656517131
24	25.849252256354475	23.72855742444518	24.498422192307615	25.92376812689272
25	25.870776854048316	23.536526198368307	24.148411355443187	26.444285592140194
26	25.56908451362363	23.74819302742224	24.452788056781195	26.229934402172937
27	25.666666003861504	23.572914201859966	24.410534227590063	26.349885566688474
28	25.509481759270493	23.95598244626804	24.477991223134023	26.056544571327443
29	25.28394573216441	24.388363394861535	24.872542567004633	25.455148305969423
30	25.616060829606713	24.378321896630233	24.03363007120516	25.9719872025579
31	25.31491530344215	24.658937032846637	24.32756759121359	25.698580072497627
32	24.76273232149497	24.069421550049412	24.7931053681253	26.37474076033032
33	24.50413888684524	24.16486549363409	25.526234159785094	25.80476145973558
34	25.868042782747715	22.811649330997607	25.315611248864116	26.004696637390563
35	24.78704070087669	23.134766848341563	25.84875515248164	26.229437298300102
36	24.38677266246846	23.50376705314836	25.9180514323551	26.191408852028086
37	24.531678441400402	22.283675307657585	26.62881054973723	26.55583570120478
38	24.160242427616705	22.444736962456727	26.92220125548554	26.472819354441025
39	23.81192174391992	21.935901438220924	28.037503504582308	26.214673313276847
40	23.06189142058368	21.528425393656555	29.0421007211983	26.36758246456147
41	22.286608220507325	21.492932177136005	29.68137630166649	26.53908330069018
42	22.221587033940263	21.541350094350314	30.123301644618454	26.11376122709097
43	21.528027710558288	21.113840763710623	30.360519612736198	26.99761191299489
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	69.0
1	84.5
2	100.0
3	278.0
4	456.0
5	456.0
6	608.5
7	761.0
8	770.5
9	780.0
10	1167.0
11	1554.0
12	1554.0
13	2482.0
14	3410.0
15	5714.5
16	8019.0
17	7823.0
18	7627.0
19	7627.0
20	8894.5
21	10162.0
22	10803.0
23	11444.0
24	14453.0
25	17462.0
26	17462.0
27	20977.0
28	24492.0
29	29296.5
30	34101.0
31	40290.5
32	46480.0
33	46480.0
34	53521.5
35	60563.0
36	69252.5
37	77942.0
38	83374.0
39	88806.0
40	88806.0
41	95229.5
42	101653.0
43	107381.5
44	113110.0
45	117421.0
46	121732.0
47	121732.0
48	128155.5
49	134579.0
50	139331.0
51	144083.0
52	148783.5
53	153484.0
54	153484.0
55	151872.5
56	150261.0
57	147602.0
58	144943.0
59	139902.0
60	134861.0
61	134861.0
62	125029.0
63	115197.0
64	104713.0
65	94229.0
66	82673.5
67	71118.0
68	71118.0
69	62729.0
70	54340.0
71	45652.5
72	36965.0
73	27922.0
74	18879.0
75	18879.0
76	14817.0
77	10755.0
78	8887.5
79	7020.0
80	5749.0
81	4478.0
82	4478.0
83	3566.0
84	2654.0
85	2112.5
86	1571.0
87	1233.5
88	896.0
89	896.0
90	686.0
91	476.0
92	291.0
93	106.0
94	65.0
95	24.0
96	24.0
97	14.5
98	5.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2011652.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.57134901178084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.20016797248384	57.834039970214334
2	7.363681480998969	9.656930568043414
3	1.7326928111251905	3.4084501514548062
4	0.7274024702942522	1.9078704500678383
5	0.4105231358607072	1.345927790946658
6	0.2547275633760838	1.0021697976632231
7	0.1768693448744841	0.811829307957488
8	0.1431374754032075	0.7508573885063132
9	0.11010989310351156	0.6498048807306214
>10	0.7423288620510843	9.542326676862608
>50	0.07782379137206571	3.5370523159744858
>100	0.05589756116779551	6.973008121284602
>500	0.003953396233139185	1.709967674821284
>1k	6.842416557356257E-4	0.869764905472344
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4096	0.20361374631397477	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2897	0.14401099196083617	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2885	0.14341446731343194	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.971038728368525E-5	0.0
3	0.0	0.0	0.0	4.971038728368525E-5	0.0
4	0.0	0.0	0.0	9.94207745673705E-5	0.0
5	0.0	0.0	0.0	1.4913116185105574E-4	0.0
6	0.0	0.0	0.0	1.4913116185105574E-4	0.0
7	0.0	0.0	0.0	1.4913116185105574E-4	0.0
8	0.0	0.0	0.0	1.4913116185105574E-4	0.0
9	0.0	0.0	0.0	1.98841549134741E-4	0.0
10	0.0	0.0	0.0	2.982623237021115E-4	0.0
11	0.0	0.0	0.0	2.982623237021115E-4	0.0
12	0.0	0.0	0.0	5.468142601205377E-4	0.0
13	0.0	0.0	0.0	5.468142601205377E-4	0.0
14	0.0	0.0	0.0	5.468142601205377E-4	0.0
15	0.0	0.0	0.0	5.468142601205377E-4	0.0
16	0.0	0.0	0.0	6.959454219715935E-4	0.0
17	0.0	0.0	0.0	9.94207745673705E-4	0.0
18	0.0	0.0	0.0	0.0010936285202410754	0.0
19	0.0	0.0	0.0	0.0013421804566595018	0.0
20	0.0	0.0	0.0	0.001590732393077928	0.0
21	0.0	0.0	0.0	0.002137546653198466	0.0
22	0.0	0.0	0.0	0.003181464786155856	0.0
23	0.0	9.94207745673705E-5	0.0	0.004623066017382728	0.0
24	0.0	9.94207745673705E-5	0.0	0.0068600334451485645	0.0
25	0.0	9.94207745673705E-5	0.0	0.009345552809332826	0.0
26	0.0	9.94207745673705E-5	0.0	0.012477307208204998	0.0
27	0.0	9.94207745673705E-5	0.0	0.03867468130670713	0.0
28	0.0	9.94207745673705E-5	0.0	0.13531167418619125	0.0
29	0.0	1.98841549134741E-4	0.0	0.30044958074259365	0.0
30	0.0	1.98841549134741E-4	0.0	0.533193614004808	0.0
31	0.0	1.98841549134741E-4	0.0	1.1585502860335684	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGTCG	30	3.60043E-4	30.833334	5
GGTATCA	1665	0.0	24.0	1
CTTATAC	2595	0.0	18.1079	37
CGTATAC	65	0.0015804911	17.076923	3
CGAATTA	110	7.8147605E-7	16.818182	15
GTACTAT	220	0.0	15.977273	1
CCGTATA	70	0.0025934004	15.857142	2
TATACAC	900	0.0	15.827778	37
TCTTATA	4360	0.0	15.529817	37
TTACTCG	120	1.9371582E-6	15.416667	19
TATGCCG	160	1.0977601E-8	15.03125	33
TCTATCG	100	1.0940115E-4	14.8	31
GGCTACG	150	8.110146E-8	14.8	1
CGAACGA	125	2.9612347E-6	14.799999	16
GTATCAA	2715	0.0	14.581952	2
CGTATGC	205	9.276846E-11	14.439024	31
ATGCGTT	180	3.3342076E-9	14.388889	36
GTCCTAT	235	3.6379788E-12	14.170212	1
TACGACG	170	2.4394467E-8	14.1470585	5
GCTACGA	170	2.4394467E-8	14.1470585	2
>>END_MODULE