FastQCFastQC Report
Fri 10 Feb 2017
ERR1632169.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632169.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596658
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2700.028.0925941
AATACTA502.6999923E-422.25
CAATACT502.6999923E-422.24
TAATACT705.0951403E-621.1428574
TAGACTG806.948576E-720.81255
GTATCAA3700.020.5000022
GTTATTC555.139111E-420.1818183
GATTTAT701.217951E-418.51
GCGTTAT609.2297216E-418.51
AACGATG852.7195634E-517.41176418
AAACGAT752.0651676E-417.26666617
TAAACGA650.0015787317.07692316
TTAGACT803.379155E-416.18754
CCCCTAT700.002590535415.8571421
CTAAGTC700.002590535415.8571424
TATGCCG700.002590535415.85714233
ATAAACG855.358784E-415.23529415
CGTATGC750.004101330414.831
CTCTTAT8000.014.5687537
TCGTATG908.2657934E-414.38888930
Produced by FastQC (version 0.11.5)