##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632169.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	596658
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.819588440949424	34.0	31.0	34.0	31.0	34.0
2	32.96937273949231	34.0	33.0	34.0	31.0	34.0
3	32.97101019344415	34.0	33.0	34.0	31.0	34.0
4	36.40188349104512	37.0	37.0	37.0	35.0	37.0
5	36.323595761726146	37.0	37.0	37.0	35.0	37.0
6	36.358813591705804	37.0	37.0	37.0	35.0	37.0
7	36.34659553714188	37.0	37.0	37.0	35.0	37.0
8	36.3369283576186	37.0	37.0	37.0	35.0	37.0
9	38.12038219549558	39.0	39.0	39.0	37.0	39.0
10	38.06016344371483	39.0	38.0	39.0	37.0	39.0
11	38.16699013505224	39.0	39.0	39.0	37.0	39.0
12	38.13236225777581	39.0	38.0	39.0	37.0	39.0
13	38.15786262817225	39.0	39.0	39.0	37.0	39.0
14	39.58373138380781	41.0	39.0	41.0	37.0	41.0
15	39.6183894291202	41.0	39.0	41.0	37.0	41.0
16	39.494137345011715	41.0	39.0	41.0	37.0	41.0
17	39.54000113968136	41.0	39.0	41.0	37.0	41.0
18	39.553524799801565	41.0	39.0	41.0	37.0	41.0
19	39.60148359696845	41.0	39.0	41.0	37.0	41.0
20	39.577079331878565	41.0	39.0	41.0	37.0	41.0
21	39.55447341693231	41.0	39.0	41.0	37.0	41.0
22	39.53865028207114	41.0	39.0	41.0	37.0	41.0
23	39.496865876264124	41.0	39.0	41.0	37.0	41.0
24	39.45224232307285	41.0	39.0	41.0	37.0	41.0
25	39.39441019813695	41.0	39.0	41.0	37.0	41.0
26	39.31183525570763	40.0	39.0	41.0	36.0	41.0
27	39.253733294450086	40.0	39.0	41.0	36.0	41.0
28	39.19766767561987	40.0	39.0	41.0	36.0	41.0
29	39.06040646400451	40.0	39.0	41.0	36.0	41.0
30	39.02527578612874	40.0	39.0	41.0	35.0	41.0
31	38.96506541435797	40.0	39.0	41.0	35.0	41.0
32	38.90751150575372	40.0	38.0	41.0	35.0	41.0
33	38.77436990704893	40.0	38.0	41.0	35.0	41.0
34	38.78279014108585	40.0	38.0	41.0	35.0	41.0
35	38.76248370088057	40.0	38.0	41.0	35.0	41.0
36	38.736264325627076	40.0	38.0	41.0	35.0	41.0
37	38.69866489680856	40.0	38.0	41.0	35.0	41.0
38	38.62789571245169	40.0	38.0	41.0	35.0	41.0
39	38.44005443654489	40.0	38.0	41.0	34.0	41.0
40	38.44807578210633	40.0	38.0	41.0	34.0	41.0
41	38.444601429964905	40.0	38.0	41.0	34.0	41.0
42	38.31369394192318	40.0	38.0	41.0	34.0	41.0
43	37.498117849756476	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	2.0
18	3.0
19	17.0
20	18.0
21	52.0
22	113.0
23	191.0
24	342.0
25	500.0
26	799.0
27	1136.0
28	1664.0
29	2367.0
30	3349.0
31	4525.0
32	6359.0
33	8711.0
34	13114.0
35	20574.0
36	33162.0
37	59928.0
38	143290.0
39	296440.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.8908051178397	17.30069822243228	11.534748549420272	27.27374811030775
2	20.700803475357745	19.322627032571425	32.094097456164164	27.88247203590667
3	21.388802295452336	20.664266631805823	28.220354038662016	29.726577034079828
4	16.109563602599813	14.852562104254027	31.98800652970378	37.04986776344237
5	17.40947075208914	33.360484565697604	32.43315266031797	16.79689202189529
6	37.52182992602127	31.87906639984715	14.63468184454076	15.964421829590822
7	31.14430712401409	27.875600427715707	19.99721783668366	20.98287461158654
8	28.596448886967075	30.741396243744322	19.062679122713515	21.599475746575088
9	27.10313110693228	13.377847946394752	18.67954506601772	40.83947588065525
10	18.33294785287384	24.9193005038062	32.19147317223602	24.556278471083935
11	38.322791280767206	19.804477606937308	19.881741299035628	21.990989813259855
12	23.38877547942037	23.43721193715663	27.495650774815722	25.678361808607274
13	32.62220568566917	18.20842090443772	22.07965031894318	27.089723090949924
14	24.51689242413577	19.303520609796568	22.673457826761727	33.50612913930593
15	27.362408615991068	25.430481113133485	20.33074223424474	26.876368036630698
16	27.171344388242513	23.770401134318153	22.26216693650299	26.796087540936348
17	25.856856021372376	24.263816122468818	23.54397326441613	26.33535459174267
18	26.04641184732292	23.284360554957782	24.330185801581475	26.33904179613782
19	26.730220662423033	23.5474928686115	23.89928568794854	25.82300078101693
20	26.78653432954892	23.310673786323154	23.881855267171478	26.020936616956448
21	27.16832758464648	23.12044755957349	23.298103771339697	26.413121084440334
22	27.337100985824375	23.200057654468726	23.04368666807451	26.41915469163239
23	26.910223276986144	23.152961998330703	23.25285171739925	26.683963007283907
24	26.812512360514734	23.513470028056275	22.888153682679192	26.785863928749805
25	27.063074659184995	22.91765131784037	23.293243365546093	26.726030657428545
26	26.867652826242168	23.30547818012999	23.24598010920829	26.58088888441955
27	26.561112060845577	23.119441958374814	23.330953410496466	26.988492570283146
28	26.407087477248275	23.137877980350552	23.64604178608181	26.808992756319366
29	26.298650147990976	23.458664762728397	23.78045714630492	26.46222794297571
30	26.26412450683641	23.57464410097577	23.628276164905188	26.532955227282628
31	26.8592728162532	23.337154617888302	23.310003385524038	26.493569180334465
32	26.103060714848368	23.37084225804397	23.47844158630237	27.047655440805286
33	25.897918070318337	23.20072805526784	24.053645471945405	26.847708402468417
34	26.59211809780477	22.702452661323573	23.78565275249808	26.919776488373575
35	26.217531651297726	22.94714895300155	24.33202940377905	26.50328999192167
36	26.12753034401617	23.048044273268772	24.07777990071364	26.746645482001412
37	26.02981942754476	22.30909499244123	24.754717107622792	26.906368472391218
38	25.375173047206275	22.495466414595967	25.370312641412667	26.75904789678509
39	25.357910226628988	22.203842066979742	25.840096001394436	26.59815170499683
40	25.061928273818502	21.96115697769912	26.452339531188723	26.524575217293663
41	24.57018928766563	22.106131150508332	26.65614137412052	26.66753818770552
42	24.342923416764712	22.26199933630321	26.603012110790438	26.79206513614164
43	23.987275792832747	21.912385319563302	26.98581096708667	27.114527920517283
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	12.5
2	13.0
3	36.0
4	59.0
5	59.0
6	69.5
7	80.0
8	75.0
9	70.0
10	112.0
11	154.0
12	154.0
13	280.5
14	407.0
15	543.0
16	679.0
17	767.5
18	856.0
19	856.0
20	1143.0
21	1430.0
22	1657.0
23	1884.0
24	2414.5
25	2945.0
26	2945.0
27	3837.0
28	4729.0
29	5813.0
30	6897.0
31	8449.0
32	10001.0
33	10001.0
34	12031.5
35	14062.0
36	16420.5
37	18779.0
38	21177.5
39	23576.0
40	23576.0
41	26221.5
42	28867.0
43	31428.0
44	33989.0
45	35809.0
46	37629.0
47	37629.0
48	39591.0
49	41553.0
50	43471.5
51	45390.0
52	46547.0
53	47704.0
54	47704.0
55	47120.5
56	46537.0
57	45517.0
58	44497.0
59	43289.5
60	42082.0
61	42082.0
62	39401.0
63	36720.0
64	33375.5
65	30031.0
66	26887.5
67	23744.0
68	23744.0
69	20775.0
70	17806.0
71	15239.0
72	12672.0
73	10315.0
74	7958.0
75	7958.0
76	6480.0
77	5002.0
78	4122.5
79	3243.0
80	2682.0
81	2121.0
82	2121.0
83	1652.5
84	1184.0
85	937.5
86	691.0
87	518.0
88	345.0
89	345.0
90	266.0
91	187.0
92	121.0
93	55.0
94	32.5
95	10.0
96	10.0
97	8.5
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	596658.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.71448926400389
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.48126291513773	68.64509383232841
2	6.422409601658181	9.85383744870884
3	1.6464242357955312	3.7891378308279613
4	0.7137439460324851	2.1901800914062743
5	0.39444945899366646	1.512999439358088
6	0.26899573411123584	1.2381522213923548
7	0.18663423237475407	1.0022284871066074
8	0.1287228325748405	0.7899925086075815
9	0.09950821675949396	0.6870349823648733
>10	0.6300474955623716	8.629132321766479
>50	0.02429881699506054	1.227598981808151
>100	0.0035025140045833055	0.4346118543243773
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	1.6760019977943814E-4	0.0	0.0	1.6760019977943814E-4
11	0.0	1.6760019977943814E-4	0.0	0.0	1.6760019977943814E-4
12	0.0	1.6760019977943814E-4	0.0	0.0	1.6760019977943814E-4
13	0.0	1.6760019977943814E-4	0.0	0.0	1.6760019977943814E-4
14	0.0	1.6760019977943814E-4	0.0	0.0	1.6760019977943814E-4
15	0.0	1.6760019977943814E-4	0.0	0.0	1.6760019977943814E-4
16	0.0	1.6760019977943814E-4	0.0	0.0	3.352003995588763E-4
17	0.0	1.6760019977943814E-4	0.0	1.6760019977943814E-4	3.352003995588763E-4
18	0.0	1.6760019977943814E-4	0.0	5.028005993383144E-4	3.352003995588763E-4
19	0.0	1.6760019977943814E-4	0.0	5.028005993383144E-4	3.352003995588763E-4
20	0.0	1.6760019977943814E-4	0.0	6.704007991177526E-4	3.352003995588763E-4
21	0.0	1.6760019977943814E-4	0.0	8.380009988971906E-4	3.352003995588763E-4
22	0.0	3.352003995588763E-4	0.0	0.001173201398456067	3.352003995588763E-4
23	0.0	3.352003995588763E-4	0.0	0.002178802597132696	3.352003995588763E-4
24	0.0	3.352003995588763E-4	0.0	0.005363206392942021	3.352003995588763E-4
25	0.0	3.352003995588763E-4	0.0	0.006704007991177525	3.352003995588763E-4
26	0.0	3.352003995588763E-4	0.0	0.01173201398456067	3.352003995588763E-4
27	0.0	3.352003995588763E-4	0.0	0.031006036959196057	3.352003995588763E-4
28	0.0	3.352003995588763E-4	0.0	0.07407928830251166	3.352003995588763E-4
29	0.0	3.352003995588763E-4	0.0	0.12921975402994682	3.352003995588763E-4
30	0.0	3.352003995588763E-4	0.0	0.20983545012385654	3.352003995588763E-4
31	0.0	3.352003995588763E-4	0.0	0.3910112660854292	3.352003995588763E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	270	0.0	28.092594	1
AATACTA	50	2.6999923E-4	22.2	5
CAATACT	50	2.6999923E-4	22.2	4
TAATACT	70	5.0951403E-6	21.142857	4
TAGACTG	80	6.948576E-7	20.8125	5
GTATCAA	370	0.0	20.500002	2
GTTATTC	55	5.139111E-4	20.181818	3
GATTTAT	70	1.217951E-4	18.5	1
GCGTTAT	60	9.2297216E-4	18.5	1
AACGATG	85	2.7195634E-5	17.411764	18
AAACGAT	75	2.0651676E-4	17.266666	17
TAAACGA	65	0.00157873	17.076923	16
TTAGACT	80	3.379155E-4	16.1875	4
CCCCTAT	70	0.0025905354	15.857142	1
CTAAGTC	70	0.0025905354	15.857142	4
TATGCCG	70	0.0025905354	15.857142	33
ATAAACG	85	5.358784E-4	15.235294	15
CGTATGC	75	0.0041013304	14.8	31
CTCTTAT	800	0.0	14.56875	37
TCGTATG	90	8.2657934E-4	14.388889	30
>>END_MODULE