FastQCFastQC Report
Fri 10 Feb 2017
ERR1632168.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632168.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2833903
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA30400.1072725495544484No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT28860.10183834803096647No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT303.6007987E-430.8333346
GTCTAGA9150.023.0491811
CTAGAAC10350.021.8067653
TAGAACT10350.021.4492764
GGTATCA15800.020.958861
TCTAGAC7950.020.7106913
ACTGATC7850.020.2675158
CTAGACA8750.019.451434
GCAATTA11050.019.08597417
CTTATAC22700.018.537
TAGGACG1306.9849193E-1018.54
GTTCTAG9500.018.3052641
GAATCTA5500.017.49090813
CGAATTA752.0686138E-417.26666615
TCTAGAA12450.017.2369462
AGAACTG14300.017.2062935
TACACCG1401.873559E-917.1785725
CAATTAC12400.016.8588718
AACGGAG9200.016.69021625
ACATGTA7350.016.6122468
Produced by FastQC (version 0.11.5)