##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632168.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2833903
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64819332207207	34.0	31.0	34.0	31.0	34.0
2	32.80292550591887	34.0	31.0	34.0	31.0	34.0
3	32.80831206996146	34.0	31.0	34.0	31.0	34.0
4	36.29446385426742	37.0	37.0	37.0	35.0	37.0
5	36.196166911852664	37.0	35.0	37.0	35.0	37.0
6	36.229417873512254	37.0	36.0	37.0	35.0	37.0
7	36.220301471151274	37.0	36.0	37.0	35.0	37.0
8	36.199345566873674	37.0	36.0	37.0	35.0	37.0
9	37.96884402888878	39.0	38.0	39.0	35.0	39.0
10	37.88329381774888	39.0	38.0	39.0	35.0	39.0
11	38.00261723848699	39.0	38.0	39.0	35.0	39.0
12	37.96699675324103	39.0	38.0	39.0	35.0	39.0
13	37.98872861915175	39.0	38.0	39.0	35.0	39.0
14	39.35671933725325	40.0	39.0	41.0	37.0	41.0
15	39.37090895489366	40.0	39.0	41.0	37.0	41.0
16	39.272305721120304	40.0	39.0	41.0	36.0	41.0
17	39.32237518362484	40.0	39.0	41.0	36.0	41.0
18	39.345100026359404	40.0	39.0	41.0	36.0	41.0
19	39.385494845801006	40.0	39.0	41.0	37.0	41.0
20	39.357697846397706	40.0	39.0	41.0	37.0	41.0
21	39.33098133563499	40.0	39.0	41.0	36.0	41.0
22	39.313761974210124	40.0	39.0	41.0	36.0	41.0
23	39.27668554640014	40.0	39.0	41.0	36.0	41.0
24	39.22296105406572	40.0	39.0	41.0	36.0	41.0
25	39.15681623541808	40.0	39.0	41.0	36.0	41.0
26	39.08193505564587	40.0	39.0	41.0	36.0	41.0
27	39.009130870040366	40.0	39.0	41.0	36.0	41.0
28	38.9501528457396	40.0	39.0	41.0	35.0	41.0
29	38.82230866758672	40.0	38.0	41.0	35.0	41.0
30	38.78001505344396	40.0	38.0	41.0	35.0	41.0
31	38.708748676295556	40.0	38.0	41.0	35.0	41.0
32	38.635168176186696	40.0	38.0	41.0	35.0	41.0
33	38.50727142036972	40.0	38.0	41.0	35.0	41.0
34	38.508727715803964	40.0	38.0	41.0	34.0	41.0
35	38.49719556385663	40.0	38.0	41.0	34.0	41.0
36	38.46631800735593	40.0	38.0	41.0	34.0	41.0
37	38.44311008527815	40.0	38.0	41.0	34.0	41.0
38	38.3827855081843	40.0	38.0	41.0	34.0	41.0
39	38.20168156778831	40.0	38.0	41.0	34.0	41.0
40	38.216687021397696	40.0	38.0	41.0	34.0	41.0
41	38.224898311621814	40.0	38.0	41.0	34.0	41.0
42	38.10093676459639	40.0	38.0	41.0	34.0	41.0
43	37.23632177953868	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	2.0
12	0.0
13	1.0
14	2.0
15	2.0
16	3.0
17	14.0
18	24.0
19	61.0
20	159.0
21	315.0
22	603.0
23	1111.0
24	1770.0
25	2927.0
26	4672.0
27	6662.0
28	9893.0
29	13976.0
30	19615.0
31	27174.0
32	36996.0
33	51558.0
34	74575.0
35	110189.0
36	175337.0
37	318323.0
38	755617.0
39	1222316.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.83022284107819	17.103408267678887	11.232106391785464	27.834262499457463
2	20.12743555442794	19.707131824907204	32.72320894540145	27.44222367526341
3	21.29049582854459	19.96896153467497	28.18399218321869	30.556550453561748
4	16.134885350698312	14.215236019016883	31.3966638942829	38.2532147360019
5	17.28252519581651	33.74378022112966	32.10875601599631	16.864938567057518
6	38.33723313747859	31.455275639286175	14.204579338107198	16.00291188512804
7	32.14178466941176	27.685068966721865	19.321444664831507	20.851701699034862
8	28.483579007467792	31.314939149293398	19.0765880130689	21.12489383016991
9	27.598192316391916	13.067243303669883	18.265621653246424	41.06894272669177
10	18.104536393800352	25.530866793958722	31.596953036148378	24.767643776092548
11	38.59292290526528	19.745135948548697	19.387007953342085	22.274933192843932
12	23.867859979681732	23.502145274555975	27.081872597615376	25.548122148146916
13	32.58047293785285	18.073025082368734	22.677487549856153	26.669014429922267
14	24.831160417276102	18.6971819430658	22.548760490390816	33.922897149267285
15	27.243240153244486	25.837299300646492	20.440290299279827	26.4791702468292
16	27.402596348569446	23.56393285161842	21.85251224195041	27.180958557861718
17	25.668909627464316	24.41604388011869	23.284812500639575	26.63023399177742
18	26.453057849898183	22.889597844386348	24.02506366661103	26.632280639104444
19	26.627763900175832	23.162048948040916	23.553664327960412	26.656522823822833
20	26.65860475817274	23.609558972201942	23.940268950630987	25.791567318994336
21	27.325458916554307	23.05167114047305	23.85801490029828	25.764855042674363
22	27.384494105832136	22.860909494785105	22.85498127494131	26.89961512444145
23	26.449529147610207	23.31688840443727	23.385098219663835	26.848484228288687
24	26.660898414659922	23.052235732839126	22.886880743624605	27.399985108876347
25	27.128769051022566	23.29561032964078	23.206969328166842	26.368651291169808
26	27.251567890644107	22.61051278043038	22.985260963413356	27.152658365512156
27	26.70144320394876	22.983814195475286	23.516612953936672	26.79812964663928
28	26.04588089288871	22.982649723720254	23.697317798103885	27.27415158528715
29	26.08035631424223	23.225353867087193	23.419150196742798	27.275139621927778
30	25.71630009919182	23.51696582416547	24.356832255726466	26.409901820916243
31	27.01062809842115	23.15770864422671	23.3018561326905	26.52980712466164
32	26.110456144758658	23.25446566096299	23.740967845406143	26.894110348872207
33	26.300900207240684	22.716550284184038	23.97379162236675	27.008757886208524
34	26.65578179634236	22.125386789879542	23.841818156796474	27.377013256981623
35	25.74085986711613	22.592833981967626	24.58196346169929	27.084342689216957
36	25.84933217544849	21.674877368773735	25.404398103957686	27.07139235182009
37	26.035682943276463	21.68182891228105	25.240736891841394	27.04175125260109
38	25.319074082634447	21.167555840831533	26.528078060540533	26.98529201599349
39	25.271013157472222	21.198502559897076	27.042668715195965	26.487815567434737
40	24.672051231111297	21.6115018756817	26.967401495393457	26.749045397813546
41	24.586480200627896	20.794889592198462	27.96207209632793	26.65655811084571
42	23.81125959498261	21.196455912570052	27.888181070417723	27.104103422029617
43	22.697212995645934	21.1148017416263	28.36303853731056	27.82494672541721
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	50.0
1	65.0
2	80.0
3	218.5
4	357.0
5	357.0
6	457.5
7	558.0
8	526.5
9	495.0
10	750.0
11	1005.0
12	1005.0
13	1761.5
14	2518.0
15	3776.0
16	5034.0
17	4953.0
18	4872.0
19	4872.0
20	5998.0
21	7124.0
22	7131.0
23	7138.0
24	8716.0
25	10294.0
26	10294.0
27	12886.5
28	15479.0
29	19286.5
30	23094.0
31	28211.5
32	33329.0
33	33329.0
34	42325.0
35	51321.0
36	62942.5
37	74564.0
38	83848.5
39	93133.0
40	93133.0
41	105398.0
42	117663.0
43	133864.5
44	150066.0
45	171300.5
46	192535.0
47	192535.0
48	203416.0
49	214297.0
50	229246.0
51	244195.0
52	250181.0
53	256167.0
54	256167.0
55	254783.0
56	253399.0
57	243988.5
58	234578.0
59	231413.5
60	228249.0
61	228249.0
62	213015.5
63	197782.0
64	169182.5
65	140583.0
66	124144.5
67	107706.0
68	107706.0
69	86216.0
70	64726.0
71	53725.5
72	42725.0
73	33242.5
74	23760.0
75	23760.0
76	18662.5
77	13565.0
78	11022.5
79	8480.0
80	7117.0
81	5754.0
82	5754.0
83	4437.0
84	3120.0
85	2608.0
86	2096.0
87	1615.0
88	1134.0
89	1134.0
90	895.0
91	656.0
92	396.0
93	136.0
94	92.0
95	48.0
96	48.0
97	40.5
98	33.0
99	19.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2833903.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.92596785967063
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.15183098370993	35.26446856419826
2	8.819826729178327	7.571991974091456
3	2.8958436581892717	3.7292067539419103
4	1.4868653973845092	2.5530054503913537
5	0.8787388556718332	1.8860357937806427
6	0.582507641862965	1.50028225875733
7	0.45027582915961306	1.3529968039344467
8	0.31431576936653965	1.0793846890892582
9	0.26327483854655864	1.0171194531938594
>10	1.7287443481161264	14.525200622315573
>50	0.21343812841677365	6.4322498302607345
>100	0.19646673546241275	16.88819755848148
>500	0.014644361266305262	4.19000568504448
>1k	0.0032267236688465254	2.0098545625192172
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	3040	0.1072725495544484	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	2886	0.10183834803096647	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.528702287975276E-5	0.0
4	0.0	0.0	3.528702287975276E-5	7.057404575950552E-5	0.0
5	0.0	1.7643511439876383E-4	3.528702287975276E-5	7.057404575950552E-5	0.0
6	0.0	1.7643511439876383E-4	3.528702287975276E-5	7.057404575950552E-5	0.0
7	0.0	1.7643511439876383E-4	3.528702287975276E-5	7.057404575950552E-5	0.0
8	0.0	1.7643511439876383E-4	3.528702287975276E-5	7.057404575950552E-5	0.0
9	0.0	1.7643511439876383E-4	3.528702287975276E-5	2.4700916015826937E-4	0.0
10	0.0	2.117221372785166E-4	3.528702287975276E-5	2.822961830380221E-4	0.0
11	0.0	2.117221372785166E-4	3.528702287975276E-5	3.175832059177749E-4	0.0
12	0.0	2.117221372785166E-4	3.528702287975276E-5	3.881572516772804E-4	0.0
13	0.0	2.117221372785166E-4	3.528702287975276E-5	4.940183203165387E-4	0.0
14	0.0	2.4700916015826937E-4	3.528702287975276E-5	6.351664118355498E-4	0.0
15	0.0	2.4700916015826937E-4	3.528702287975276E-5	8.468885491140664E-4	0.0
16	0.0	2.4700916015826937E-4	3.528702287975276E-5	0.0015173419838293688	0.0
17	0.0	2.822961830380221E-4	3.528702287975276E-5	0.00268181373886121	0.0
18	0.0	2.822961830380221E-4	3.528702287975276E-5	0.002999396944778985	0.0
19	0.0	3.175832059177749E-4	7.057404575950552E-5	0.0033875541964562654	0.0
20	0.0	3.175832059177749E-4	7.057404575950552E-5	0.003740424425253793	0.0
21	0.0	3.175832059177749E-4	7.057404575950552E-5	0.005046044271804645	0.0
22	0.0	3.175832059177749E-4	7.057404575950552E-5	0.006281090072595992	0.0
23	0.0	5.293053431962915E-4	7.057404575950552E-5	0.007727858010665855	0.0
24	0.0	5.293053431962915E-4	7.057404575950552E-5	0.010550819841046076	0.0
25	0.0	5.293053431962915E-4	7.057404575950552E-5	0.012562180145191984	0.0
26	0.0	5.293053431962915E-4	7.057404575950552E-5	0.01662018777636355	0.0
27	0.0	5.293053431962915E-4	7.057404575950552E-5	0.03355795875864488	0.0
28	0.0	5.99879388955797E-4	7.057404575950552E-5	0.092487286967832	0.0
29	0.0	6.351664118355498E-4	7.057404575950552E-5	0.1842335464551892	0.0
30	0.0	6.351664118355498E-4	7.057404575950552E-5	0.3086203021063177	0.0
31	0.0	6.351664118355498E-4	7.057404575950552E-5	0.6870383354687863	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACCGT	30	3.6007987E-4	30.833334	6
GTCTAGA	915	0.0	23.049181	1
CTAGAAC	1035	0.0	21.806765	3
TAGAACT	1035	0.0	21.449276	4
GGTATCA	1580	0.0	20.95886	1
TCTAGAC	795	0.0	20.710691	3
ACTGATC	785	0.0	20.267515	8
CTAGACA	875	0.0	19.45143	4
GCAATTA	1105	0.0	19.085974	17
CTTATAC	2270	0.0	18.5	37
TAGGACG	130	6.9849193E-10	18.5	4
GTTCTAG	950	0.0	18.305264	1
GAATCTA	550	0.0	17.490908	13
CGAATTA	75	2.0686138E-4	17.266666	15
TCTAGAA	1245	0.0	17.236946	2
AGAACTG	1430	0.0	17.206293	5
TACACCG	140	1.873559E-9	17.178572	5
CAATTAC	1240	0.0	16.85887	18
AACGGAG	920	0.0	16.690216	25
ACATGTA	735	0.0	16.612246	8
>>END_MODULE