FastQCFastQC Report
Fri 10 Feb 2017
ERR1632167.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632167.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266815
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA4610.17277889174146882No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC4470.16753181043044807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3950.14804265127522814No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG3780.14167119539756012No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG3150.11805932949796676No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA2840.10644079230927796No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT2770.1038172516537676TruSeq Adapter, Index 10 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG451.0746771E-732.8888933
CCGTCTT451.0746771E-732.8888937
GTATTGT250.005488874429.66
GTCTTAC250.005488874429.61
GCCGTCT502.7075475E-729.636
ATACAGT405.9233076E-527.756
ATGCCGT556.231494E-726.9090934
TGTATTG358.851483E-426.428575
CGGTGAT358.851483E-426.4285714
GGAGTGT451.3194949E-424.6666666
ATCAATA400.001927323823.1252
TTATACT400.001927323823.1254
CTAGGCC400.001927323823.1254
GGTATCA2650.023.0377371
TCTAGGC652.6700473E-622.7692283
ATTGCCG603.712499E-521.58333211
CGTATGC705.0777344E-621.14285731
GTATGCC705.0777344E-621.14285732
TCGTATG705.0777344E-621.14285730
CTTATTG450.003818227220.55555528
Produced by FastQC (version 0.11.5)