FastQCFastQC Report
Fri 10 Feb 2017
ERR1632166.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632166.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298155
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15630.5242239774613876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11300.3789975012996596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9340.31325988160520535No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT3900.13080444735120994TruSeq Adapter, Index 7 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.11470543844644564No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT3180.1066559339940635No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTTC250.005489896529.68
CTATTGG250.005489896529.61
ATAACGC250.005489896529.63
GACCTAT250.005489896529.66
TAGATAA358.853935E-426.428574
CCGCTTA601.331864E-624.66666625
GTACTAT451.3200038E-424.6666661
AGTCGGT1500.024.66666611
GGTATCA8700.023.6034491
TACCAAC400.001927852223.1250027
CAGTCGG1650.022.42424210
GCAGTCG1700.021.7647069
CGCTTAT705.0810413E-621.14285726
CGGTGAT1700.020.67647214
GATAACC450.003819265220.5555556
CGATTAA450.003819265220.55555524
GTATAGA450.003819265220.5555551
CTCTATG2100.020.2619061
GTGATTC1650.020.18181816
TCGGTGA1650.020.18181813
Produced by FastQC (version 0.11.5)