##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632165.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	766601
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73452943578211	34.0	31.0	34.0	31.0	34.0
2	32.89429442434852	34.0	31.0	34.0	31.0	34.0
3	32.900259717897576	34.0	31.0	34.0	31.0	34.0
4	36.35522912179869	37.0	37.0	37.0	35.0	37.0
5	36.265683191125504	37.0	37.0	37.0	35.0	37.0
6	36.29409823363132	37.0	37.0	37.0	35.0	37.0
7	36.28084362008398	37.0	37.0	37.0	35.0	37.0
8	36.26650891402438	37.0	37.0	37.0	35.0	37.0
9	38.047263178628775	39.0	38.0	39.0	35.0	39.0
10	37.974369978645996	39.0	38.0	39.0	35.0	39.0
11	38.08307059343779	39.0	38.0	39.0	37.0	39.0
12	38.04297150668992	39.0	38.0	39.0	35.0	39.0
13	38.06812279138691	39.0	38.0	39.0	35.0	39.0
14	39.46540377588863	40.0	39.0	41.0	37.0	41.0
15	39.489448878882236	40.0	39.0	41.0	37.0	41.0
16	39.373957247642515	40.0	39.0	41.0	37.0	41.0
17	39.414032854118375	40.0	39.0	41.0	37.0	41.0
18	39.427969700013435	40.0	39.0	41.0	37.0	41.0
19	39.473475771620436	41.0	39.0	41.0	37.0	41.0
20	39.445928194719286	40.0	39.0	41.0	37.0	41.0
21	39.41912937760321	40.0	39.0	41.0	37.0	41.0
22	39.39962901170231	40.0	39.0	41.0	37.0	41.0
23	39.35939556562018	40.0	39.0	41.0	36.0	41.0
24	39.30362470176794	40.0	39.0	41.0	36.0	41.0
25	39.23407222270777	40.0	39.0	41.0	36.0	41.0
26	39.14742219224864	40.0	39.0	41.0	36.0	41.0
27	39.083694125105495	40.0	39.0	41.0	36.0	41.0
28	39.018462016094425	40.0	39.0	41.0	35.0	41.0
29	38.873755708641134	40.0	38.0	41.0	35.0	41.0
30	38.83186429446348	40.0	38.0	41.0	35.0	41.0
31	38.76112867058613	40.0	38.0	41.0	35.0	41.0
32	38.699259458310124	40.0	38.0	41.0	35.0	41.0
33	38.554249211780316	40.0	38.0	41.0	35.0	41.0
34	38.55876133738411	40.0	38.0	41.0	35.0	41.0
35	38.52867006434899	40.0	38.0	41.0	35.0	41.0
36	38.50070245147084	40.0	38.0	41.0	35.0	41.0
37	38.46198348293311	40.0	38.0	41.0	34.0	41.0
38	38.39755752992756	40.0	38.0	41.0	34.0	41.0
39	38.21972055867394	40.0	38.0	41.0	34.0	41.0
40	38.218071721795305	40.0	38.0	41.0	34.0	41.0
41	38.21410616474542	40.0	38.0	41.0	34.0	41.0
42	38.08133696668801	40.0	37.0	41.0	34.0	41.0
43	37.22542756923093	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	2.0
14	0.0
15	3.0
16	2.0
17	5.0
18	6.0
19	15.0
20	51.0
21	95.0
22	150.0
23	298.0
24	514.0
25	767.0
26	1177.0
27	1757.0
28	2510.0
29	3583.0
30	4842.0
31	6843.0
32	9360.0
33	13197.0
34	19299.0
35	29126.0
36	46753.0
37	83751.0
38	194010.0
39	348482.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.516509892369044	17.190559365302157	11.726960961438872	27.565969780889926
2	20.365092140500728	19.310827927435522	32.11709872541257	28.20698120665118
3	21.07067431427822	20.595981481892146	28.13627949872228	30.19706470510735
4	15.999979128647107	14.806789972880285	32.165233283024676	37.02799761544793
5	17.530110187698686	33.2731107838367	32.26828558793949	16.928493440525124
6	37.47307921591545	32.18140858151764	14.632253284303046	15.71325891826387
7	31.80298486435577	27.725505184574505	19.870049739042866	20.601460212026858
8	29.08879586642856	30.76450461191676	18.875268881725958	21.271430639928724
9	27.563099969866983	13.36523171767321	18.175034992127586	40.89663332033222
10	18.521108112303533	24.696419649856967	31.483131381253088	25.29934085658641
11	38.84928404737275	19.761518703993342	19.724211160695067	21.664986087938836
12	23.76086125637718	23.274950071810498	27.177371279192176	25.786817392620147
13	32.880077119648945	17.856094630714022	22.049932102880117	27.213896146756916
14	24.481183823136156	18.713776788707552	22.72485947709434	34.08017991106195
15	27.522792169590176	25.20098460607278	20.119071068261064	27.157152156075977
16	27.459786773040996	23.6820718992018	21.923529971914984	26.934611355842218
17	26.15728390649112	24.045494331471	23.145547683866834	26.651674078171045
18	26.437612265050532	23.01797153930141	23.852303871244622	26.69211232440344
19	26.975440939941375	23.277037207099912	23.599369163358773	26.148152689599936
20	27.102625746640037	23.02579829663671	23.35726146978676	26.51431448693649
21	27.537923900438425	22.97244590080107	23.000230889341392	26.48939930941911
22	27.684023370697403	22.863653973840368	22.75721007408026	26.69511258138197
23	26.955613154691942	22.819041457029147	23.056844434066743	27.168500954212167
24	26.792294818295304	23.253556935094004	22.76738485861615	27.18676338799454
25	27.08240662352384	22.63485176773837	23.007014079031986	27.275727529705808
26	26.83625510532859	23.17633292938569	23.110327275857976	26.87708468942775
27	26.709331190541103	23.02514606685877	23.048756784820267	27.21676595777986
28	26.549404448989762	22.912832099097184	23.44687784127597	27.090885610637084
29	26.324385175599822	23.263862165585486	23.636154922834695	26.775597735979993
30	26.23672549344444	23.481706911418065	23.38674225574973	26.89482533938776
31	26.951047546246354	23.07954203033912	23.140068953732122	26.829341469682404
32	26.151544284445233	23.12363276332799	23.33730323858174	27.387519713645037
33	25.969441730443872	22.788908441288232	24.072235752366616	27.169414075901283
34	26.646064902080745	22.313041595301858	23.82934538306107	27.211548119556326
35	26.14097816204258	22.579803574480074	24.492271729361168	26.786946534116186
36	25.827516530763724	22.514319704774714	24.356607935549263	27.301555828912306
37	25.769598526482483	21.543540903286065	25.194070970426598	27.49278959980485
38	24.997880253221687	21.640984032110577	26.13328185066286	27.227853864004874
39	24.878000420035978	21.342523685724384	26.623628197719544	27.15584769652009
40	24.65285070069045	21.129114102381813	27.38621525408915	26.83181994283858
41	23.85112985764433	20.983275524034013	27.977787662682413	27.187806955639243
42	23.572888634374337	21.218600027915436	28.25655066977476	26.95196066793547
43	22.87695946131038	20.79321576674176	28.706589216554633	27.623235555393222
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	18.5
2	22.0
3	48.0
4	74.0
5	74.0
6	96.5
7	119.0
8	119.0
9	119.0
10	183.0
11	247.0
12	247.0
13	462.0
14	677.0
15	994.5
16	1312.0
17	1329.0
18	1346.0
19	1346.0
20	1652.0
21	1958.0
22	2105.5
23	2253.0
24	2834.5
25	3416.0
26	3416.0
27	4408.0
28	5400.0
29	6862.0
30	8324.0
31	9977.5
32	11631.0
33	11631.0
34	14351.0
35	17071.0
36	19946.0
37	22821.0
38	25936.0
39	29051.0
40	29051.0
41	32200.0
42	35349.0
43	38454.5
44	41560.0
45	44713.5
46	47867.0
47	47867.0
48	50753.0
49	53639.0
50	55860.0
51	58081.0
52	59546.0
53	61011.0
54	61011.0
55	60952.5
56	60894.0
57	59978.0
58	59062.0
59	57742.5
60	56423.0
61	56423.0
62	52770.5
63	49118.0
64	44526.0
65	39934.0
66	35600.5
67	31267.0
68	31267.0
69	26988.0
70	22709.0
71	19608.5
72	16508.0
73	13404.5
74	10301.0
75	10301.0
76	8483.0
77	6665.0
78	5512.0
79	4359.0
80	3608.5
81	2858.0
82	2858.0
83	2188.5
84	1519.0
85	1198.0
86	877.0
87	662.5
88	448.0
89	448.0
90	337.0
91	226.0
92	141.0
93	56.0
94	33.0
95	10.0
96	10.0
97	7.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	766601.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.16465920563768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.22850882013238	67.06830456892486
2	6.453229814060714	9.701096394990682
3	1.7243791959697448	3.8883712381907207
4	0.750895952692563	2.2576335353211654
5	0.4464296206623658	1.6777865148194415
6	0.2680866372646734	1.209038443655075
7	0.2049420678160242	1.07830804790031
8	0.14779523672319167	0.8887182880412199
9	0.10984403970712998	0.7430750829321262
>10	0.6213654449340208	8.487721563343156
>50	0.03269741782273302	1.6187857895060724
>100	0.011477935977248917	1.2432536282341997
>500	3.4781623721801555E-4	0.1379069041409837
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	1.3044595558836996E-4	0.0	0.0	0.0
10	0.0	1.3044595558836996E-4	0.0	0.0	0.0
11	0.0	1.3044595558836996E-4	0.0	0.0	0.0
12	0.0	1.3044595558836996E-4	0.0	0.0	0.0
13	0.0	2.6089191117673993E-4	0.0	0.0	0.0
14	0.0	2.6089191117673993E-4	0.0	2.6089191117673993E-4	1.3044595558836996E-4
15	0.0	2.6089191117673993E-4	0.0	3.913378667651099E-4	1.3044595558836996E-4
16	0.0	2.6089191117673993E-4	0.0	7.826757335302198E-4	1.3044595558836996E-4
17	0.0	2.6089191117673993E-4	0.0	0.0010435676447069597	1.3044595558836996E-4
18	0.0	2.6089191117673993E-4	0.0	0.0010435676447069597	1.3044595558836996E-4
19	0.0	2.6089191117673993E-4	0.0	0.0014349055114720695	1.3044595558836996E-4
20	0.0	2.6089191117673993E-4	0.0	0.0019566893338255494	1.3044595558836996E-4
21	0.0	2.6089191117673993E-4	0.0	0.002869811022944139	2.6089191117673993E-4
22	0.0	2.6089191117673993E-4	0.0	0.004043824623239468	2.6089191117673993E-4
23	0.0	2.6089191117673993E-4	0.0	0.006261405868241758	3.913378667651099E-4
24	0.0	2.6089191117673993E-4	0.0	0.010044338580304487	3.913378667651099E-4
25	0.0	2.6089191117673993E-4	0.0	0.012261919825306776	3.913378667651099E-4
26	0.0	2.6089191117673993E-4	0.0	0.017349312093253206	3.913378667651099E-4
27	0.0	2.6089191117673993E-4	0.0	0.043438503210927196	3.913378667651099E-4
28	0.0	2.6089191117673993E-4	0.0	0.11570556260688415	3.913378667651099E-4
29	0.0	2.6089191117673993E-4	0.0	0.22071455685552197	3.913378667651099E-4
30	0.0	2.6089191117673993E-4	0.0	0.36381377013596383	5.217838223534799E-4
31	0.0	2.6089191117673993E-4	0.0	0.7117131336901465	5.217838223534799E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTTAT	20	0.0018415536	37.0	9
TCATTAG	50	2.7009656E-4	22.199999	5
GGTATCA	380	0.0	21.421053	1
TACGCTA	45	0.0038246629	20.555555	9
GGACCGT	65	6.898297E-5	19.923077	6
TATACTG	95	1.6742888E-7	19.473684	5
TACTGCG	50	0.0070327986	18.499998	7
GTGCTAC	75	2.0661355E-4	17.266666	1
TAAGGTG	75	2.0661355E-4	17.266666	5
CGAAGTT	65	0.0015792848	17.076923	22
CCTAAAC	110	7.7992627E-7	16.818182	3
TCTTATA	1025	0.0	16.604877	37
ATAACGC	70	0.0025914377	15.857142	3
TCTATAC	70	0.0025914377	15.857142	3
CTTATGC	85	5.361258E-4	15.235293	3
CCGAAGT	85	5.361258E-4	15.235293	21
CTAGCAC	85	5.361258E-4	15.235293	3
TTACACT	110	1.4505347E-5	15.136364	4
TTATCGA	75	0.004102749	14.799999	10
TAGCACC	125	2.9554503E-6	14.799999	4
>>END_MODULE