FastQCFastQC Report
Fri 10 Feb 2017
ERR1632164.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632164.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428076
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT108670.7609538988121081No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT98740.6914197843812234No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT67960.4758850369308076No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38670.2707839078592456No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14830.10384601379758501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA851.2457403E-619.5882368
GGTATCA46700.019.0546041
GACCGTT1205.176844E-918.57
TGTATCG500.00703569618.510
CCGTTAT953.6076253E-617.5263169
CGTATGC1757.2759576E-1216.91428631
TATGCCG1655.4569682E-1116.81818233
CTTATAC9550.016.65968737
GTAGAAC3650.016.219183
TTATACC1455.347829E-815.3103454
CCGTTTA1101.4522797E-515.13636427
TAGAACT4000.014.84
ATAGCGA750.00410505414.86
TTAAGGT1001.0936583E-414.84
GGACCGT1508.104507E-814.86
TATACCA2006.184564E-1114.85
GTCGGGT1252.9597759E-614.79999924
AGTCGGT5000.014.79999911
TCTTATA14550.014.49484537
ACCGTGC908.2757464E-414.3888888
Produced by FastQC (version 0.11.5)