##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632164.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1428076
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27869315078469	33.0	31.0	34.0	30.0	34.0
2	32.42702209126125	34.0	31.0	34.0	31.0	34.0
3	32.41811150106857	34.0	31.0	34.0	30.0	34.0
4	36.04591002159549	37.0	35.0	37.0	35.0	37.0
5	35.844963433318675	37.0	35.0	37.0	35.0	37.0
6	35.87402491183943	37.0	35.0	37.0	35.0	37.0
7	35.85934221988185	37.0	35.0	37.0	35.0	37.0
8	35.844963433318675	37.0	35.0	37.0	35.0	37.0
9	37.54509213795344	39.0	37.0	39.0	35.0	39.0
10	37.40340990255421	39.0	37.0	39.0	35.0	39.0
11	37.53888518538229	39.0	37.0	39.0	35.0	39.0
12	37.48215571160078	39.0	37.0	39.0	35.0	39.0
13	37.5350863679524	39.0	37.0	39.0	35.0	39.0
14	38.75954921166661	40.0	38.0	41.0	35.0	41.0
15	38.75089280962638	40.0	38.0	41.0	35.0	41.0
16	38.62135348538873	40.0	38.0	41.0	35.0	41.0
17	38.69445813808229	40.0	38.0	41.0	35.0	41.0
18	38.73076783028354	40.0	38.0	41.0	35.0	41.0
19	38.7869945297029	40.0	38.0	41.0	35.0	41.0
20	38.76334312739658	40.0	38.0	41.0	35.0	41.0
21	38.74147594385733	40.0	38.0	41.0	35.0	41.0
22	38.698216341427205	40.0	38.0	41.0	35.0	41.0
23	38.64609656628919	40.0	38.0	41.0	35.0	41.0
24	38.5701741363905	40.0	38.0	41.0	35.0	41.0
25	38.492484293553005	40.0	38.0	41.0	34.0	41.0
26	38.35660567084665	40.0	38.0	41.0	34.0	41.0
27	38.24654640229232	40.0	38.0	41.0	34.0	41.0
28	38.15283570342195	40.0	38.0	41.0	34.0	41.0
29	37.9767211268868	40.0	37.0	41.0	33.0	41.0
30	37.87258241158034	40.0	37.0	41.0	33.0	41.0
31	37.743333688123045	40.0	37.0	41.0	33.0	41.0
32	37.60847952069778	40.0	37.0	41.0	33.0	41.0
33	37.41003419986051	39.0	36.0	41.0	33.0	41.0
34	37.369977508199845	39.0	36.0	41.0	32.0	41.0
35	37.29861435945986	39.0	36.0	41.0	32.0	41.0
36	37.22930782395335	39.0	36.0	41.0	32.0	41.0
37	37.14157579848692	39.0	36.0	41.0	32.0	41.0
38	37.01901719516328	39.0	35.0	41.0	31.0	41.0
39	36.758890283150194	39.0	35.0	40.0	31.0	41.0
40	36.70438688137046	39.0	35.0	40.0	31.0	41.0
41	36.621838753679775	39.0	35.0	40.0	31.0	41.0
42	36.40625989093017	39.0	35.0	40.0	30.0	41.0
43	35.46787145782157	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	3.0
11	4.0
12	2.0
13	2.0
14	8.0
15	6.0
16	10.0
17	24.0
18	59.0
19	88.0
20	220.0
21	392.0
22	694.0
23	1208.0
24	1994.0
25	3118.0
26	4772.0
27	6739.0
28	9900.0
29	13573.0
30	18469.0
31	24162.0
32	31503.0
33	41898.0
34	58845.0
35	83481.0
36	131643.0
37	235277.0
38	414388.0
39	345589.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.52094986541332	17.693105969150103	12.420557449323425	25.365386716113147
2	20.730829451653836	20.480842756267872	31.885978057190233	26.90234973488806
3	21.472456647965515	21.793027822048685	28.917858713401806	27.81665681658399
4	15.789845918564557	15.637753172800329	31.57261938440251	36.9997815242326
5	16.266991392614962	34.965155916071694	31.895011189880652	16.8728415014327
6	35.74018469605259	32.199476778546796	15.275237452348476	16.785101073052132
7	30.213448023774646	29.40410734442705	19.84915368649848	20.533290945299832
8	29.626434447466384	30.610275643593198	18.697884426319046	21.065405482621376
9	27.32235539285024	13.013733162660813	19.123211929897288	40.54069951459166
10	19.01236348765752	25.969346169251494	30.579044812741056	24.439245530349925
11	36.94061100389615	20.830614056954953	19.39161501208619	22.837159927062707
12	22.929311885361845	25.78126094129444	27.04141796374983	24.24800920959389
13	32.71338500191867	18.571980762928582	23.06901033278341	25.64562390236934
14	24.01412809962495	20.25018276338234	24.334209103717168	31.40148003327554
15	27.557426915654347	25.88454676081665	21.301107223985277	25.256919099543722
16	25.93489422131595	24.057263058828802	23.775415314030905	26.23242740582434
17	24.491833767950723	25.707735442651515	24.505208406275298	25.295222383122468
18	24.46228351992471	23.325579310905024	26.08922774418168	26.12290942498859
19	25.00952330268137	24.514381587534555	26.41196967108193	24.064125438702142
20	25.796946381004933	23.387830899756036	26.559580862643166	24.255641856595865
21	26.705511471378273	23.202616667460273	25.585963212041936	24.505908649119515
22	26.364493206243928	23.818410224665914	25.27617577775973	24.540920791330436
23	25.308036827171666	23.70770183099499	25.429318887790288	25.55494245404306
24	25.011133861223072	24.319854125410693	25.580291245003767	25.088720768362467
25	25.461249961486644	23.825342628823677	25.537086261515498	25.176321148174186
26	25.550811021262177	23.82555270167694	25.567196703816887	25.056439573244
27	25.026259106658188	24.306339438517277	25.498642929367904	25.16875852545663
28	24.604642890154306	24.002924214117456	26.06962094454357	25.322811951184672
29	24.4885426265829	24.363969424596448	26.348807766533433	24.798680182287217
30	24.465994806999067	24.726975315039258	26.291317829023104	24.515712048938575
31	25.139698447421566	24.44589783737	25.604449623129298	24.809954092079135
32	24.431262761925836	24.622849204103982	25.973827723454495	24.972060310515687
33	24.14710421574202	24.403393096725946	26.28004391923119	25.169458768300846
34	24.601141675933214	24.212576921676437	25.872712656749357	25.313568745640993
35	24.243317582537625	24.328397088110158	26.304412370209988	25.12387295914223
36	24.100047896610544	24.50954991190945	26.028376641019108	25.3620255504609
37	24.351995271960316	23.806225999176515	26.43521773350998	25.40656099535319
38	23.79313145798963	23.759799898604836	27.247429408518876	25.199639234886657
39	23.67927197151972	23.397354202437405	27.68564138043073	25.23773244561214
40	23.529840148563522	23.346656620515997	27.802721983984046	25.320781246936434
41	22.822734924471806	23.651192233466567	28.248146457191353	25.277926384870277
42	22.7774992367353	23.626683733918924	28.277136510941997	25.31868051840378
43	22.29538203849095	23.45386379996583	28.478876474361307	25.771877687181917
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	54.0
1	121.0
2	188.0
3	605.5
4	1023.0
5	1023.0
6	1469.0
7	1915.0
8	1993.5
9	2072.0
10	3052.0
11	4032.0
12	4032.0
13	7465.0
14	10898.0
15	16327.0
16	21756.0
17	19197.5
18	16639.0
19	16639.0
20	18331.0
21	20023.0
22	15364.0
23	10705.0
24	11220.5
25	11736.0
26	11736.0
27	13555.5
28	15375.0
29	18029.0
30	20683.0
31	23937.0
32	27191.0
33	27191.0
34	31511.5
35	35832.0
36	39844.5
37	43857.0
38	49112.0
39	54367.0
40	54367.0
41	60285.0
42	66203.0
43	71172.0
44	76141.0
45	81399.5
46	86658.0
47	86658.0
48	93055.5
49	99453.0
50	99956.0
51	100459.0
52	99161.5
53	97864.0
54	97864.0
55	96487.5
56	95111.0
57	93867.0
58	92623.0
59	89380.5
60	86138.0
61	86138.0
62	83049.0
63	79960.0
64	75076.0
65	70192.0
66	62917.0
67	55642.0
68	55642.0
69	49508.0
70	43374.0
71	37635.0
72	31896.0
73	25277.5
74	18659.0
75	18659.0
76	15261.5
77	11864.0
78	9529.0
79	7194.0
80	6071.5
81	4949.0
82	4949.0
83	3846.0
84	2743.0
85	2084.5
86	1426.0
87	1084.0
88	742.0
89	742.0
90	536.0
91	330.0
92	203.0
93	76.0
94	49.0
95	22.0
96	22.0
97	14.5
98	7.0
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1428076.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.264945260495274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.75313594824898	49.87113208055548
2	10.308757529121253	12.425134163924218
3	2.799848386681251	5.061981292830948
4	1.2089517739098938	2.914296499090337
5	0.643636755110724	1.9394366907195295
6	0.4129379849474096	1.4931411035300917
7	0.29010038741915345	1.223801877750463
8	0.21560941158137764	1.0394951509279458
9	0.17481228557082001	0.9481547538708782
>10	1.0552009837829799	12.130468945762885
>50	0.09140269648184145	3.81425243891707
>100	0.04397710524525956	4.400835752303445
>500	9.307153708890417E-4	0.3546259325370908
>1k	3.490182640833906E-4	0.45257224091081866
>5k	2.3267884272226043E-4	1.1687651829563668
>10k+	1.1633942136113022E-4	0.7619058934125278
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10867	0.7609538988121081	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9874	0.6914197843812234	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6796	0.4758850369308076	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3867	0.2707839078592456	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1483	0.10384601379758501	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.002428442183749E-5	0.0
2	0.0	7.002428442183749E-5	0.0	7.002428442183749E-5	0.0
3	0.0	7.002428442183749E-5	0.0	7.002428442183749E-5	0.0
4	0.0	7.002428442183749E-5	0.0	7.002428442183749E-5	0.0
5	0.0	7.002428442183749E-5	0.0	7.002428442183749E-5	0.0
6	0.0	7.002428442183749E-5	0.0	7.002428442183749E-5	0.0
7	0.0	7.002428442183749E-5	0.0	7.002428442183749E-5	0.0
8	0.0	7.002428442183749E-5	0.0	7.002428442183749E-5	0.0
9	0.0	7.002428442183749E-5	0.0	7.002428442183749E-5	0.0
10	0.0	7.002428442183749E-5	0.0	7.002428442183749E-5	0.0
11	0.0	7.002428442183749E-5	0.0	7.002428442183749E-5	0.0
12	0.0	7.002428442183749E-5	0.0	1.4004856884367498E-4	0.0
13	0.0	7.002428442183749E-5	0.0	2.1007285326551248E-4	0.0
14	0.0	7.002428442183749E-5	0.0	2.8009713768734995E-4	0.0
15	0.0	7.002428442183749E-5	0.0	5.601942753746999E-4	0.0
16	0.0	7.002428442183749E-5	0.0	9.80339981905725E-4	0.0
17	0.0	7.002428442183749E-5	0.0	0.0013304614040149125	0.0
18	0.0	7.002428442183749E-5	0.0	0.0016105585417022623	0.0
19	0.0	7.002428442183749E-5	0.0	0.0017506071105459374	0.0
20	0.0	7.002428442183749E-5	0.0	0.002100728532655125	0.0
21	0.0	7.002428442183749E-5	0.0	0.003011044230139012	0.0
22	0.0	7.002428442183749E-5	0.0	0.004901699909528624	0.0
23	0.0	7.002428442183749E-5	0.0	0.007352549864292937	0.0
24	0.0	7.002428442183749E-5	0.0	0.010993812654228486	0.0
25	0.0	7.002428442183749E-5	0.0	0.013724759746680148	0.0
26	0.0	7.002428442183749E-5	0.0	0.01918665393158347	0.0
27	0.0	7.002428442183749E-5	0.0	0.034732045073231396	0.0
28	0.0	7.002428442183749E-5	0.0	0.0808780485072223	0.0
29	0.0	7.002428442183749E-5	0.0	0.15272296432402757	0.0
30	0.0	7.002428442183749E-5	0.0	0.26371145513264	0.0
31	0.0	7.002428442183749E-5	0.0	0.5189499718502376	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTTA	85	1.2457403E-6	19.588236	8
GGTATCA	4670	0.0	19.054604	1
GACCGTT	120	5.176844E-9	18.5	7
TGTATCG	50	0.007035696	18.5	10
CCGTTAT	95	3.6076253E-6	17.526316	9
CGTATGC	175	7.2759576E-12	16.914286	31
TATGCCG	165	5.4569682E-11	16.818182	33
CTTATAC	955	0.0	16.659687	37
GTAGAAC	365	0.0	16.21918	3
TTATACC	145	5.347829E-8	15.310345	4
CCGTTTA	110	1.4522797E-5	15.136364	27
TAGAACT	400	0.0	14.8	4
ATAGCGA	75	0.004105054	14.8	6
TTAAGGT	100	1.0936583E-4	14.8	4
GGACCGT	150	8.104507E-8	14.8	6
TATACCA	200	6.184564E-11	14.8	5
GTCGGGT	125	2.9597759E-6	14.799999	24
AGTCGGT	500	0.0	14.799999	11
TCTTATA	1455	0.0	14.494845	37
ACCGTGC	90	8.2757464E-4	14.388888	8
>>END_MODULE