FastQCFastQC Report
Fri 10 Feb 2017
ERR1632163.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632163.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2792339
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG65140.2332811309801568No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT61050.218633912286438No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA57480.20584893166624824No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC55760.1996892211153445No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG54700.19589312042699686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52700.18873066629803903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50280.18006409680200003No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC49680.1779153605633127No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG49090.17580243659527012No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG45350.16240864737411898No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA45290.16219377375025024No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT43220.15478063372677886No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC43010.1540285760432383No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA42670.15281095884131546No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG41930.15016085081360106No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC41880.14998178946037713No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA40320.14439507523979No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG39960.1431058334965776No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG39490.1414226567762725No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA38990.13963204324403306No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT38960.13952460643209869No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC38910.13934554507887473No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA38880.13923810826694036No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG38250.13698193521631863No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36140.12942554611026813No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC33400.1196129839535959No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA33200.1188967385407001No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG33060.11839536675167306No Hit
CTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCGCC32030.11470670287525979No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG31450.112629591177862No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC30740.11008691996208196No Hit
GCCAGGGACTTCTGCAGAAACTGACGGAGTCTGGGGTCCGAGG30550.10940648681983098No Hit
GATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAACGCAA30430.10897673957209351No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG28600.10242309404409708No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA28500.1020649713376492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22100.023.9411771
CTTATAC18350.017.74386837
GTATCAA31750.016.6062982
CTAGTAC957.0654525E-515.5789483
ATACGAC957.0654525E-515.5789483
CGTAAGC750.004106359614.82
TCTTATA30050.014.4059937
TCTAGCG2201.8189894E-1114.29545428
CGAACGA2009.858923E-1013.87500116
ATAACGA2009.858923E-1013.87500112
GTACGAC800.006301593513.8750013
GTATACG1203.3036824E-513.8749991
CCGTTAT950.001245853513.6315799
CTCTTAT46250.013.0837
GTATAGG3550.013.0281691
TAACGAA2152.904926E-912.90697813
TCGTATG1153.5800203E-412.86956530
CGCTTAT1307.004797E-512.80769326
CTAGAGA2501.6007107E-1012.584
CGGTGAT3100.012.53225814
Produced by FastQC (version 0.11.5)