##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632163.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2792339
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.772809103765695	34.0	31.0	34.0	31.0	34.0
2	32.91992412096096	34.0	31.0	34.0	31.0	34.0
3	32.931583880037486	34.0	33.0	34.0	31.0	34.0
4	36.38223295953679	37.0	37.0	37.0	35.0	37.0
5	36.29747641672447	37.0	37.0	37.0	35.0	37.0
6	36.32689404832293	37.0	37.0	37.0	35.0	37.0
7	36.316802508577936	37.0	37.0	37.0	35.0	37.0
8	36.303048447914094	37.0	37.0	37.0	35.0	37.0
9	38.094870644287816	39.0	38.0	39.0	37.0	39.0
10	38.03344722829141	39.0	38.0	39.0	35.0	39.0
11	38.12367767667178	39.0	38.0	39.0	37.0	39.0
12	38.08559061059563	39.0	38.0	39.0	37.0	39.0
13	38.108609663797985	39.0	38.0	39.0	37.0	39.0
14	39.533447049230055	40.0	39.0	41.0	37.0	41.0
15	39.549223070694495	41.0	39.0	41.0	37.0	41.0
16	39.458452215150096	41.0	39.0	41.0	37.0	41.0
17	39.4768368024083	40.0	39.0	41.0	37.0	41.0
18	39.487657479983625	41.0	39.0	41.0	37.0	41.0
19	39.52310553983596	41.0	39.0	41.0	37.0	41.0
20	39.51214483628242	41.0	39.0	41.0	37.0	41.0
21	39.48744117386893	41.0	39.0	41.0	37.0	41.0
22	39.471890053464136	41.0	39.0	41.0	37.0	41.0
23	39.43897033991933	40.0	39.0	41.0	37.0	41.0
24	39.38586683063912	41.0	39.0	41.0	37.0	41.0
25	39.33037643352043	40.0	39.0	41.0	36.0	41.0
26	39.237806369498834	40.0	39.0	41.0	36.0	41.0
27	39.1746653970023	40.0	39.0	41.0	36.0	41.0
28	39.10631911096755	40.0	39.0	41.0	36.0	41.0
29	38.9775503619009	40.0	39.0	41.0	35.0	41.0
30	38.93595440954698	40.0	38.0	41.0	35.0	41.0
31	38.835798590357406	40.0	38.0	41.0	35.0	41.0
32	38.7717984814881	40.0	38.0	41.0	35.0	41.0
33	38.63377118609166	40.0	38.0	41.0	35.0	41.0
34	38.59426201474821	40.0	38.0	41.0	35.0	41.0
35	38.5728341007306	40.0	38.0	41.0	35.0	41.0
36	38.526177874534575	40.0	38.0	41.0	35.0	41.0
37	38.4610571997168	40.0	38.0	41.0	35.0	41.0
38	38.399905956977285	40.0	38.0	41.0	34.0	41.0
39	38.19835986962901	40.0	38.0	41.0	34.0	41.0
40	38.169096588917036	40.0	37.0	41.0	34.0	41.0
41	38.13204055811275	40.0	37.0	41.0	34.0	41.0
42	37.99819004784161	40.0	37.0	41.0	34.0	41.0
43	37.14694455078699	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	3.0
13	1.0
14	9.0
15	2.0
16	2.0
17	18.0
18	21.0
19	62.0
20	134.0
21	296.0
22	549.0
23	995.0
24	1647.0
25	2606.0
26	3991.0
27	5877.0
28	8608.0
29	12316.0
30	17442.0
31	24169.0
32	32917.0
33	45600.0
34	67119.0
35	99888.0
36	162931.0
37	314231.0
38	696414.0
39	1294486.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.15920165853788	17.468795873280428	11.212893563424785	26.159108904756906
2	20.083485565327134	19.71379549546097	33.004731875320296	27.1979870638916
3	21.69983658860905	20.228668510521107	27.777250541571064	30.294244359298784
4	14.891780689952045	15.145367378387798	32.44885381037188	37.51399812128828
5	17.394342162609913	33.44225038578769	32.046216451512514	17.11719100008989
6	37.547733280235676	31.780990775117207	15.275222671745803	15.39605327290132
7	32.08206453442794	27.787421226434184	19.41472722330634	20.71578701583153
8	28.257672152270906	31.164876470944254	19.46701313844773	21.110438238337107
9	26.858486738178993	13.006622763210341	18.95335774058952	41.18153275802114
10	17.88769200301253	25.18146972842481	32.17954553512306	24.751292733439602
11	37.66734626418927	20.478853033245606	19.63601124362049	22.217789458944633
12	22.90413162585202	23.510863115116038	27.29844048305023	26.286564775981713
13	32.07837587055153	18.272351602008207	23.223756141356763	26.425516386083498
14	24.51152958147274	19.540607354622775	23.374812298936483	32.57305076496801
15	26.128596850167547	26.16444493308298	21.455131343293203	26.25182687345627
16	27.88536778664768	23.672913639783708	22.55947433316657	25.88224424040204
17	25.442935116402417	24.53713535498376	23.564617333353866	26.45531219525996
18	25.0306642567396	23.01876670418599	25.303338885429028	26.647230153645385
19	27.303991385000177	24.014491077193707	24.412365404057315	24.269152133748804
20	27.17334822168798	23.459006947222385	24.10033308992927	25.26731174116037
21	26.03942429626202	23.824506981423102	23.874859033949676	26.2612096883652
22	27.690835532505186	22.670134249458968	23.72960446421441	25.909425753821434
23	24.838603049271597	23.534678275094823	24.356283388227578	27.27043528740601
24	25.854597167464267	24.122321824105168	23.640897469827266	26.3821835386033
25	26.161759012784625	23.44410904263415	24.25500628684411	26.139125657737118
26	26.901533087494034	23.65722786524129	23.054758036184005	26.386481011080676
27	26.76290378782805	23.320592521180274	23.05966431726234	26.856839373729336
28	25.970951234789187	23.85874351215952	24.14828572032264	26.022019532728656
29	26.461400281269576	23.9849817661824	24.53906921759858	25.014548734949443
30	26.43758512129079	23.142354850181157	23.42398254653178	26.996077481996277
31	25.298611665703913	23.031014500746508	24.414335078942777	27.2560387546068
32	25.032311621189262	23.740885329467517	24.117236481673608	27.109566567669614
33	25.69820498155847	22.846617119196488	24.554038746728104	26.90113915251694
34	26.76934999654412	23.09594214742551	24.61474054547102	25.519967310559355
35	26.84781468152685	22.747202255886553	23.672734578430486	26.732248484156113
36	25.26337239138944	22.89242101335117	23.798292399311116	28.045914195948274
37	24.977841157538535	22.57931433110378	26.274889975751513	26.16795453560617
38	25.01143306740335	21.924630211446388	25.258967482100132	27.80496923905013
39	24.18642578855934	22.792862900958657	27.018030403901534	26.002680906580466
40	25.73724035656129	21.628176235048823	26.15978933789916	26.474794070490727
41	23.78479117327803	22.5513449477302	27.088974512048857	26.574889366942912
42	23.72774222614088	23.421726373481157	26.470174287577546	26.380357112800418
43	24.18402636642614	22.00896810881487	26.36033805350998	27.446667471249015
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	283.0
1	309.0
2	335.0
3	772.5
4	1210.0
5	1210.0
6	1507.5
7	1805.0
8	1678.5
9	1552.0
10	2233.0
11	2914.0
12	2914.0
13	4929.5
14	6945.0
15	9116.5
16	11288.0
17	11033.0
18	10778.0
19	10778.0
20	12925.5
21	15073.0
22	14961.5
23	14850.0
24	17556.5
25	20263.0
26	20263.0
27	22629.5
28	24996.0
29	29889.5
30	34783.0
31	41984.0
32	49185.0
33	49185.0
34	66377.5
35	83570.0
36	92191.5
37	100813.0
38	104263.0
39	107713.0
40	107713.0
41	113609.5
42	119506.0
43	133631.5
44	147757.0
45	150911.0
46	154065.0
47	154065.0
48	157660.5
49	161256.0
50	175189.0
51	189122.0
52	211057.5
53	232993.0
54	232993.0
55	232005.5
56	231018.0
57	212224.5
58	193431.0
59	195033.5
60	196636.0
61	196636.0
62	188478.5
63	180321.0
64	149353.5
65	118386.0
66	109535.0
67	100684.0
68	100684.0
69	91979.5
70	83275.0
71	86238.5
72	89202.0
73	76342.5
74	63483.0
75	63483.0
76	40221.5
77	16960.0
78	13695.5
79	10431.0
80	8560.0
81	6689.0
82	6689.0
83	5313.5
84	3938.0
85	3191.0
86	2444.0
87	1949.0
88	1454.0
89	1454.0
90	1101.0
91	748.0
92	438.5
93	129.0
94	80.5
95	32.0
96	32.0
97	24.0
98	16.0
99	11.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2792339.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.34942246649182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.65636253429227	42.62398961715572
2	9.018258436051054	9.081282078174766
3	2.589257820712333	3.911029076691396
4	1.1001703551570332	2.215717679876473
5	0.6238991992594904	1.5706482180011023
6	0.36268392818432854	1.0956555793175722
7	0.2633197957435673	0.9280599747778242
8	0.18825699001865553	0.7582904578175538
9	0.14282123702293312	0.6471870120049161
>10	0.8633829792064605	8.423397914454322
>50	0.09440668151798176	3.3060101886218094
>100	0.07338083249612302	7.4545811383621166
>500	0.010577426816882979	3.756614806084308
>1k	0.012721499820303558	12.798564549357112
>5k	5.002837007984546E-4	1.428971709303036
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	6514	0.2332811309801568	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	6105	0.218633912286438	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	5748	0.20584893166624824	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	5576	0.1996892211153445	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	5470	0.19589312042699686	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5270	0.18873066629803903	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5028	0.18006409680200003	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	4968	0.1779153605633127	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	4909	0.17580243659527012	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	4535	0.16240864737411898	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	4529	0.16219377375025024	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	4322	0.15478063372677886	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	4301	0.1540285760432383	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	4267	0.15281095884131546	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	4193	0.15016085081360106	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	4188	0.14998178946037713	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	4032	0.14439507523979	No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG	3996	0.1431058334965776	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	3949	0.1414226567762725	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	3899	0.13963204324403306	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	3896	0.13952460643209869	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	3891	0.13934554507887473	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	3888	0.13923810826694036	No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG	3825	0.13698193521631863	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3614	0.12942554611026813	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	3340	0.1196129839535959	No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA	3320	0.1188967385407001	No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG	3306	0.11839536675167306	No Hit
CTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCGCC	3203	0.11470670287525979	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	3145	0.112629591177862	No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC	3074	0.11008691996208196	No Hit
GCCAGGGACTTCTGCAGAAACTGACGGAGTCTGGGGTCCGAGG	3055	0.10940648681983098	No Hit
GATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAACGCAA	3043	0.10897673957209351	No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG	2860	0.10242309404409708	No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA	2850	0.1020649713376492	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.581227064478919E-5	0.0
2	0.0	0.0	0.0	3.581227064478919E-5	0.0
3	0.0	0.0	0.0	3.581227064478919E-5	0.0
4	0.0	0.0	0.0	3.581227064478919E-5	0.0
5	0.0	3.581227064478919E-5	0.0	3.581227064478919E-5	0.0
6	0.0	3.581227064478919E-5	0.0	3.581227064478919E-5	0.0
7	0.0	3.581227064478919E-5	0.0	3.581227064478919E-5	0.0
8	0.0	3.581227064478919E-5	0.0	3.581227064478919E-5	0.0
9	0.0	3.581227064478919E-5	0.0	1.0743681193436756E-4	0.0
10	0.0	3.581227064478919E-5	0.0	1.0743681193436756E-4	0.0
11	0.0	3.581227064478919E-5	0.0	1.4324908257915675E-4	0.0
12	0.0	3.581227064478919E-5	0.0	1.4324908257915675E-4	0.0
13	0.0	3.581227064478919E-5	0.0	1.4324908257915675E-4	0.0
14	0.0	3.581227064478919E-5	0.0	1.7906135322394594E-4	0.0
15	0.0	3.581227064478919E-5	0.0	2.5068589451352434E-4	3.581227064478919E-5
16	0.0	3.581227064478919E-5	0.0	6.446208716062054E-4	3.581227064478919E-5
17	0.0	3.581227064478919E-5	0.0	8.236822248301513E-4	3.581227064478919E-5
18	0.0	7.162454128957838E-5	0.0	8.594944954749405E-4	3.581227064478919E-5
19	0.0	7.162454128957838E-5	0.0	0.0010027435780540974	3.581227064478919E-5
20	0.0	7.162454128957838E-5	0.0	0.0011818049312780432	7.162454128957838E-5
21	0.0	7.162454128957838E-5	0.0	0.0013250540138572	7.162454128957838E-5
22	0.0	7.162454128957838E-5	0.0	0.0018980503441738271	7.162454128957838E-5
23	0.0	1.4324908257915675E-4	0.0	0.002864981651583135	7.162454128957838E-5
24	0.0	1.4324908257915675E-4	0.0	0.00533602832607359	7.162454128957838E-5
25	0.0	1.4324908257915675E-4	0.0	0.007341515482181784	7.162454128957838E-5
26	0.0	1.4324908257915675E-4	0.0	0.01282079289083453	1.0743681193436756E-4
27	0.0	1.4324908257915675E-4	0.0	0.035060212961248616	1.0743681193436756E-4
28	0.0	1.7906135322394594E-4	0.0	0.09902092833284211	1.0743681193436756E-4
29	0.0	2.5068589451352434E-4	0.0	0.1739043862510963	1.0743681193436756E-4
30	0.0	2.5068589451352434E-4	0.0	0.2693798997901043	1.0743681193436756E-4
31	0.0	2.5068589451352434E-4	0.0	0.5839906974045773	1.0743681193436756E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2210	0.0	23.941177	1
CTTATAC	1835	0.0	17.743868	37
GTATCAA	3175	0.0	16.606298	2
CTAGTAC	95	7.0654525E-5	15.578948	3
ATACGAC	95	7.0654525E-5	15.578948	3
CGTAAGC	75	0.0041063596	14.8	2
TCTTATA	3005	0.0	14.40599	37
TCTAGCG	220	1.8189894E-11	14.295454	28
CGAACGA	200	9.858923E-10	13.875001	16
ATAACGA	200	9.858923E-10	13.875001	12
GTACGAC	80	0.0063015935	13.875001	3
GTATACG	120	3.3036824E-5	13.874999	1
CCGTTAT	95	0.0012458535	13.631579	9
CTCTTAT	4625	0.0	13.08	37
GTATAGG	355	0.0	13.028169	1
TAACGAA	215	2.904926E-9	12.906978	13
TCGTATG	115	3.5800203E-4	12.869565	30
CGCTTAT	130	7.004797E-5	12.807693	26
CTAGAGA	250	1.6007107E-10	12.58	4
CGGTGAT	310	0.0	12.532258	14
>>END_MODULE