FastQCFastQC Report
Fri 10 Feb 2017
ERR1632162.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632162.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607675
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8270.13609248364668614No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA7650.12588966141440738No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC7370.12128193524499115No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG6110.10054716748261816No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAA250.0054943329.631
TAGACGT502.7000715E-422.24
GACGTAG502.7000715E-422.26
ACGTAGA502.7000715E-422.27
GTATAGT753.7347672E-722.1999994
TATTAAT1107.4578566E-1121.8636362
CGTTATT957.1286195E-921.4210532
GCCATAT555.139261E-420.1818181
AGACGTA555.139261E-420.1818185
CGAGCAT555.139261E-420.18181818
GGACCGA555.139261E-420.1818186
TATACTG555.139261E-420.1818185
TAGAGCA656.89529E-519.9230774
TATAGTG951.67287E-719.4736845
GCGTTAT1002.869383E-718.51
ACGGGAT500.007031159518.530
GTTAATC500.007031159518.53
CGTAGAC609.229986E-418.58
TGCTATC801.614628E-518.57
TGAATAG801.614628E-518.55
Produced by FastQC (version 0.11.5)