##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632162.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	607675
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.481637388406632	31.0	31.0	34.0	30.0	34.0
2	31.662717735631713	31.0	31.0	34.0	30.0	34.0
3	31.674388447772248	31.0	31.0	34.0	30.0	34.0
4	35.521703213066196	37.0	35.0	37.0	33.0	37.0
5	34.85499321183198	37.0	35.0	37.0	32.0	37.0
6	34.82114946311762	36.0	35.0	37.0	32.0	37.0
7	34.82229645781051	35.0	35.0	37.0	32.0	37.0
8	34.74383675484428	35.0	35.0	37.0	32.0	37.0
9	36.38279343399021	38.0	35.0	39.0	32.0	39.0
10	36.08800592421936	37.0	35.0	39.0	32.0	39.0
11	36.28282716912823	37.0	35.0	39.0	32.0	39.0
12	36.178732052495164	37.0	35.0	39.0	32.0	39.0
13	36.27657876331921	38.0	35.0	39.0	32.0	39.0
14	37.29457357962727	39.0	36.0	40.0	32.0	41.0
15	37.260305261858726	39.0	36.0	40.0	32.0	41.0
16	37.12530382194429	38.0	36.0	40.0	32.0	41.0
17	37.16524457975069	38.0	36.0	40.0	32.0	41.0
18	37.14735672851442	38.0	36.0	40.0	32.0	41.0
19	37.226209733821534	39.0	36.0	40.0	32.0	41.0
20	37.18310610112313	39.0	36.0	40.0	32.0	41.0
21	37.13715226066565	39.0	36.0	40.0	32.0	41.0
22	37.11991442794257	39.0	36.0	40.0	32.0	41.0
23	37.04445632945242	39.0	36.0	40.0	31.0	41.0
24	36.950659480808	39.0	36.0	40.0	31.0	41.0
25	36.84715020364504	38.0	36.0	40.0	31.0	41.0
26	36.67480643436047	38.0	36.0	40.0	31.0	41.0
27	36.58084337845065	38.0	35.0	40.0	30.0	41.0
28	36.4536043115152	38.0	35.0	40.0	30.0	41.0
29	36.29574525856749	38.0	35.0	40.0	30.0	41.0
30	36.19923807956556	38.0	35.0	40.0	30.0	41.0
31	36.07056568066812	38.0	35.0	40.0	30.0	41.0
32	35.946461513144364	38.0	35.0	40.0	29.0	41.0
33	35.77391533303164	38.0	34.0	40.0	29.0	41.0
34	35.75973341012877	38.0	34.0	40.0	29.0	41.0
35	35.70489488624676	38.0	34.0	40.0	29.0	41.0
36	35.66191302916855	38.0	34.0	40.0	28.0	41.0
37	35.64807339449541	38.0	34.0	40.0	28.0	41.0
38	35.530105730859425	38.0	34.0	40.0	28.0	41.0
39	35.34030032500926	38.0	34.0	40.0	27.0	41.0
40	35.32492368453532	38.0	34.0	40.0	27.0	41.0
41	35.302768749742874	38.0	34.0	40.0	27.0	41.0
42	35.19353108158144	38.0	33.0	40.0	27.0	41.0
43	34.20442177150615	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	1.0
12	1.0
13	2.0
14	5.0
15	1.0
16	15.0
17	49.0
18	76.0
19	135.0
20	231.0
21	501.0
22	690.0
23	1190.0
24	1885.0
25	2685.0
26	3917.0
27	5563.0
28	7751.0
29	10876.0
30	14776.0
31	19443.0
32	26096.0
33	35214.0
34	47967.0
35	66050.0
36	90051.0
37	115348.0
38	116879.0
39	40273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.17830254659152	18.542642037273215	12.816390340231209	26.462665075904063
2	19.59830501501625	21.190768091496277	33.17217262516971	26.03875426831777
3	20.50059653597729	21.990702266836713	30.01193071954581	27.496770477640187
4	15.949315012136422	15.82655202205126	33.114246924754184	35.10988604105813
5	15.748056115522276	35.3926029538816	33.284732793022584	15.57460813757354
6	35.68568725058625	34.02608302135187	15.222117085613199	15.066112642448678
7	30.287571481466248	29.79405109639199	20.29785658452298	19.620520837618795
8	28.055128152384086	32.58337104537787	19.681408647714647	19.680092154523386
9	27.262928374542312	13.965359772904925	18.279837083967582	40.49187476858518
10	17.44337022256963	26.513679187065453	31.869173489118364	24.173777101246554
11	37.30612580738059	20.89488624676019	20.58304192208006	21.215946023779157
12	22.977084790389597	24.5583576747439	27.456452873657796	25.00810466120871
13	31.568025671617228	18.454601555107583	23.850742584440695	26.126630188834493
14	23.972518204632408	19.680750401119017	23.36939976138561	32.97733163286296
15	26.62673303986506	26.604681778911427	20.880733944954127	25.887851236269388
16	26.606327395400502	25.03953593615008	22.34796560661538	26.00617106183404
17	25.475788867404454	25.3298226848233	23.62957172830872	25.56481671946353
18	24.78249064055622	23.983050150162505	25.072283704282718	26.162175504998558
19	26.39420742995845	24.147118114123504	24.892253260377668	24.566421195540382
20	26.563705928333402	23.410704735261447	24.73904636524458	25.286542971160568
21	26.68432961698276	24.303287118936932	23.37071625457687	25.641667009503433
22	26.809067346854814	23.85255276257868	23.409223680421277	25.929156210145226
23	25.770189657300367	24.15600444316452	23.863249269757684	26.210556629777432
24	26.51088163903402	24.104825770354218	23.70675114164644	25.677541448965318
25	26.38614390916197	23.382564693298228	24.089192413707984	26.14209898383182
26	26.155428477393343	23.79347513062081	23.942074299584483	26.109022092401368
27	26.199366437651705	23.598634138314065	23.725346607973012	26.47665281606122
28	25.444028469165257	24.054469905788455	24.365162298926236	26.136339326120044
29	25.45620603118443	24.383264080306084	24.378985477434483	25.781544411074997
30	25.135639939112192	25.23223762702102	24.04327971366273	25.58884272020406
31	26.11083227053935	24.196157485498006	24.107129633438927	25.58588061052372
32	24.90821574032172	24.304439050479285	24.430657835191507	26.356687374007485
33	25.06997984119801	23.941580614637758	24.596371415641585	26.39206812852265
34	25.569589007281852	23.87493314683013	24.579092442506273	25.97638540338174
35	25.421977208211626	23.90027564076192	24.98769901674415	25.690048134282307
36	25.08989180071584	24.22906981527955	24.837783354589213	25.843255029415396
37	25.826798864524626	22.846587402805778	25.033940840087215	26.292672892582384
38	24.677006623606367	23.486073970461185	26.11527543505986	25.721643970872588
39	24.761920434442754	22.812029456535154	26.226848233019297	26.199201876002796
40	24.276299008516062	22.577858230139466	27.306372649853948	25.839470111490513
41	23.451680585839473	22.782902044678487	27.91920023038631	25.846217139095735
42	24.13938371662484	21.928991648496318	27.756613321265476	26.17501131361336
43	22.87242358168429	21.899041428395112	28.558193113094994	26.670341876825603
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	11.0
2	11.0
3	27.0
4	43.0
5	43.0
6	77.5
7	112.0
8	125.5
9	139.0
10	196.5
11	254.0
12	254.0
13	474.0
14	694.0
15	1254.0
16	1814.0
17	1733.0
18	1652.0
19	1652.0
20	1875.0
21	2098.0
22	2311.5
23	2525.0
24	3159.0
25	3793.0
26	3793.0
27	4518.5
28	5244.0
29	7211.0
30	9178.0
31	10648.0
32	12118.0
33	12118.0
34	14155.0
35	16192.0
36	18273.5
37	20355.0
38	24245.5
39	28136.0
40	28136.0
41	31407.5
42	34679.0
43	34423.5
44	34168.0
45	37593.5
46	41019.0
47	41019.0
48	43268.5
49	45518.0
50	48349.0
51	51180.0
52	54406.0
53	57632.0
54	57632.0
55	52331.5
56	47031.0
57	44654.5
58	42278.0
59	39121.0
60	35964.0
61	35964.0
62	33477.0
63	30990.0
64	27854.0
65	24718.0
66	21579.0
67	18440.0
68	18440.0
69	16044.5
70	13649.0
71	11790.0
72	9931.0
73	8181.0
74	6431.0
75	6431.0
76	5152.0
77	3873.0
78	3252.0
79	2631.0
80	2073.5
81	1516.0
82	1516.0
83	1170.0
84	824.0
85	639.0
86	454.0
87	343.0
88	232.0
89	232.0
90	172.5
91	113.0
92	70.5
93	28.0
94	17.0
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	607675.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.15139405476522
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.19605307766307	56.32855096037256
2	6.008648827556574	7.589090996914566
3	1.6501950520589392	3.126363539993937
4	0.7895940876946718	1.9945586950127627
5	0.4570719139603925	1.4432364274939247
6	0.3043079053260681	1.153048106593603
7	0.20895222456895035	0.9236936990661505
8	0.16400326746319707	0.8285627975869932
9	0.12940894467198	0.7355119733272367
>10	0.8912020669575077	11.180071344691376
>50	0.12049510353294546	5.2306150207458035
>100	0.07770488016241672	8.527868321823364
>500	0.0023626483833160553	0.9388281163778671
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	827	0.13609248364668614	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	765	0.12588966141440738	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	737	0.12128193524499115	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	611	0.10054716748261816	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.6456164890772204E-4	0.0	0.0	0.0
3	0.0	1.6456164890772204E-4	0.0	0.0	0.0
4	0.0	1.6456164890772204E-4	0.0	0.0	0.0
5	0.0	1.6456164890772204E-4	0.0	0.0	0.0
6	0.0	1.6456164890772204E-4	0.0	0.0	0.0
7	0.0	1.6456164890772204E-4	0.0	0.0	0.0
8	0.0	1.6456164890772204E-4	0.0	0.0	0.0
9	0.0	1.6456164890772204E-4	0.0	1.6456164890772204E-4	0.0
10	0.0	1.6456164890772204E-4	0.0	1.6456164890772204E-4	0.0
11	0.0	1.6456164890772204E-4	0.0	3.291232978154441E-4	0.0
12	0.0	1.6456164890772204E-4	0.0	3.291232978154441E-4	0.0
13	0.0	1.6456164890772204E-4	0.0	3.291232978154441E-4	0.0
14	0.0	1.6456164890772204E-4	0.0	3.291232978154441E-4	0.0
15	0.0	1.6456164890772204E-4	0.0	6.582465956308882E-4	0.0
16	0.0	1.6456164890772204E-4	0.0	0.001974739786892665	1.6456164890772204E-4
17	0.0	3.291232978154441E-4	0.0	0.0024684247336158307	1.6456164890772204E-4
18	0.0	3.291232978154441E-4	0.0	0.0026329863825235527	1.6456164890772204E-4
19	0.0	3.291232978154441E-4	0.0	0.003126671329246719	3.291232978154441E-4
20	0.0	3.291232978154441E-4	0.0	0.0034557946270621634	3.291232978154441E-4
21	0.0	3.291232978154441E-4	0.0	0.004114041222693051	3.291232978154441E-4
22	0.0	3.291232978154441E-4	0.0	0.00559509606286255	3.291232978154441E-4
23	0.0	3.291232978154441E-4	0.0	0.007405274200847492	3.291232978154441E-4
24	0.0	3.291232978154441E-4	0.0	0.010696507179001934	3.291232978154441E-4
25	0.0	3.291232978154441E-4	0.0	0.012177562019171433	3.291232978154441E-4
26	0.0	3.291232978154441E-4	0.0	0.01530423334841815	3.291232978154441E-4
27	0.0	3.291232978154441E-4	0.0	0.025013370633973752	3.291232978154441E-4
28	0.0	3.291232978154441E-4	0.0	0.06697659110544288	3.291232978154441E-4
29	0.0	6.582465956308882E-4	0.0	0.13296581231743942	3.291232978154441E-4
30	0.0	6.582465956308882E-4	0.0	0.22561402065248692	3.291232978154441E-4
31	0.0	6.582465956308882E-4	0.0	0.4604434936438063	3.291232978154441E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCAA	25	0.00549433	29.6	31
TAGACGT	50	2.7000715E-4	22.2	4
GACGTAG	50	2.7000715E-4	22.2	6
ACGTAGA	50	2.7000715E-4	22.2	7
GTATAGT	75	3.7347672E-7	22.199999	4
TATTAAT	110	7.4578566E-11	21.863636	2
CGTTATT	95	7.1286195E-9	21.421053	2
GCCATAT	55	5.139261E-4	20.181818	1
AGACGTA	55	5.139261E-4	20.181818	5
CGAGCAT	55	5.139261E-4	20.181818	18
GGACCGA	55	5.139261E-4	20.181818	6
TATACTG	55	5.139261E-4	20.181818	5
TAGAGCA	65	6.89529E-5	19.923077	4
TATAGTG	95	1.67287E-7	19.473684	5
GCGTTAT	100	2.869383E-7	18.5	1
ACGGGAT	50	0.0070311595	18.5	30
GTTAATC	50	0.0070311595	18.5	3
CGTAGAC	60	9.229986E-4	18.5	8
TGCTATC	80	1.614628E-5	18.5	7
TGAATAG	80	1.614628E-5	18.5	5
>>END_MODULE