FastQCFastQC Report
Fri 10 Feb 2017
ERR1632161.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632161.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171350
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18461.0773271082579516No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14510.8468047855266997No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9790.5713451998832798No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6250.3647505106507149No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT4810.2807119929967902RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT3540.2065946892325649No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.16749343449080828No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC2840.16574263203968487No Hit
CGCTAACCCTGTGGGAAGCTGGGGGGACCACCCTCCAAGGCTA2410.14064779690691567No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG2350.1371461920046688No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA2220.12955938138313394No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC2080.12138896994455793No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG1740.10154654216515903No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTT303.5868218E-430.83333237
TTCCTGT303.5868218E-430.8333321
TTCACGT250.005483462529.59999837
CTAGAAT358.8385027E-426.4285717
TCCTGCT358.8385027E-426.428572
GCCGTCT809.640644E-1025.437536
TACTGGT1051.8189894E-1224.6666662
GAATCGC400.001924524823.12520
ACTAGAA400.001924524823.12516
AACTAGA400.001924524823.12515
ATGCCCC400.001924524823.12537
TATACAC802.6917405E-823.1253
ATTTCGG400.001924524823.12529
GTACTGG1053.8198777E-1122.904761
GGTATCA8000.022.1999991
GGTTCAC1151.3278623E-1020.9130426
ACTGGTT1151.3278623E-1020.9130423
TCTACTG450.003812735420.5555553
CAACTAG450.003812735420.55555514
AGAATCG450.003812735420.55555519
Produced by FastQC (version 0.11.5)