##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632161.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	171350
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7482054274876	34.0	31.0	34.0	31.0	34.0
2	32.874829296761014	34.0	31.0	34.0	31.0	34.0
3	32.83840093376131	34.0	31.0	34.0	31.0	34.0
4	36.30217683104756	37.0	37.0	37.0	35.0	37.0
5	36.24496060694485	37.0	35.0	37.0	35.0	37.0
6	36.30067697694777	37.0	37.0	37.0	35.0	37.0
7	36.29353953895536	37.0	37.0	37.0	35.0	37.0
8	36.30730668222935	37.0	37.0	37.0	35.0	37.0
9	38.07716953603735	39.0	39.0	39.0	37.0	39.0
10	37.99848847388386	39.0	38.0	39.0	35.0	39.0
11	38.10033265246572	39.0	38.0	39.0	37.0	39.0
12	38.07987160782025	39.0	38.0	39.0	37.0	39.0
13	38.109851181791655	39.0	39.0	39.0	37.0	39.0
14	39.4614998540998	40.0	39.0	41.0	37.0	41.0
15	39.50056609279253	40.0	39.0	41.0	37.0	41.0
16	39.32508316311643	40.0	39.0	41.0	37.0	41.0
17	39.39940472716662	40.0	39.0	41.0	37.0	41.0
18	39.4645170703239	40.0	39.0	41.0	37.0	41.0
19	39.50662970528159	41.0	39.0	41.0	37.0	41.0
20	39.514881820834546	41.0	39.0	41.0	37.0	41.0
21	39.49426320396849	41.0	39.0	41.0	37.0	41.0
22	39.45062737087832	41.0	39.0	41.0	37.0	41.0
23	39.40399182958856	41.0	39.0	41.0	36.0	41.0
24	39.325789320105045	40.0	39.0	41.0	36.0	41.0
25	39.247837758972864	40.0	39.0	41.0	36.0	41.0
26	39.1222877152028	40.0	39.0	41.0	35.0	41.0
27	39.02470382258535	40.0	39.0	41.0	35.0	41.0
28	38.95311351035892	40.0	38.0	41.0	35.0	41.0
29	38.77139772395682	40.0	38.0	41.0	35.0	41.0
30	38.66497811496936	40.0	38.0	41.0	35.0	41.0
31	38.52498395097753	40.0	38.0	41.0	35.0	41.0
32	38.38689816165743	40.0	38.0	41.0	35.0	41.0
33	38.21524365334111	40.0	37.0	41.0	34.0	41.0
34	38.14153487014882	40.0	37.0	41.0	34.0	41.0
35	38.06474467464255	40.0	37.0	41.0	34.0	41.0
36	37.945713451998834	40.0	37.0	41.0	34.0	41.0
37	37.851298511817916	40.0	37.0	41.0	34.0	41.0
38	37.71785234899329	40.0	37.0	41.0	33.0	41.0
39	37.430300554420775	40.0	36.0	41.0	33.0	41.0
40	37.33229646921506	40.0	36.0	41.0	33.0	41.0
41	37.226314560840386	40.0	35.0	41.0	33.0	41.0
42	36.967184126057774	39.0	35.0	41.0	32.0	41.0
43	36.04857893201051	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	0.0
18	3.0
19	5.0
20	6.0
21	14.0
22	35.0
23	79.0
24	137.0
25	233.0
26	363.0
27	533.0
28	755.0
29	1044.0
30	1463.0
31	1904.0
32	2409.0
33	3264.0
34	4732.0
35	7017.0
36	11456.0
37	23044.0
38	42925.0
39	69928.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.672599941639916	18.549168368835716	13.53603735045229	24.242194339072075
2	20.665304931426903	19.961482346075286	32.95827254158156	26.41494018091625
3	22.67289174204844	22.30230522322731	29.593813831339368	25.430989203384886
4	15.619492267289173	16.34140647796907	31.850014590020425	36.18908666472133
5	15.627662678727749	35.732127224978115	31.367960315144437	17.272249781149693
6	32.714911000875404	31.65334111467756	16.45462503647505	19.177122847971987
7	28.242777939889113	30.259118762766267	19.80215932302305	21.695943974321562
8	30.20951269331777	30.225269915377883	19.200466880653632	20.364750510650715
9	27.857017799824916	12.569594397432157	19.917128683980156	39.65625911876277
10	20.891158447621827	24.7545958564342	28.875401225561713	25.478844470382256
11	35.568135395389554	21.9177122847972	18.891158447621827	23.622993872191422
12	21.871024219433906	26.73066822293551	26.50014590020426	24.89816165742632
13	33.51677852348993	18.38225853516195	24.344324482054276	23.756638459293843
14	24.69448497227896	20.81645754304056	24.171578640210097	30.31747884447038
15	30.81879194630873	24.09279252990954	21.404727166618034	23.683688357163703
16	25.92238109133353	23.749635249489348	24.646046104464546	25.681937554712576
17	23.574555004377007	26.691566968193754	24.769185876860227	24.96469215056901
18	23.85643419900788	22.098628538079954	27.027137437992415	27.017799824919752
19	25.023052232273123	25.069740297636418	27.512109716953603	22.395097753136856
20	26.047271666180333	22.162241027137437	27.697694776772686	24.09279252990954
21	27.609571053399474	22.05719288007003	25.770644878902832	24.562591187627664
22	27.714619200466878	22.748759848263788	25.206886489641082	24.329734461628245
23	25.73854683396557	22.82054274875985	26.078786110300555	25.36212430697403
24	25.24365334111468	22.91800408520572	27.02013422818792	24.818208345491684
25	24.844470382258535	23.743215640501898	25.884447038225854	25.527866939013716
26	25.476510067114095	22.894660052524074	26.284213597899036	25.344616282462795
27	25.242486139480597	23.404143565800993	25.95973154362416	25.39363875109425
28	24.661803326524655	23.37262912168077	26.53574555004377	25.4298220017508
29	24.533994747592647	23.84301138021593	26.920922089290926	24.702071782900497
30	23.657426320396848	24.967026553837176	27.86110300554421	23.51444412022177
31	23.558797782316894	24.704989786985703	26.511234315728043	25.22497811496936
32	23.844762182667058	24.451123431572803	27.565217391304348	24.13889699445579
33	23.881529034140648	23.927049897869857	27.487598482637875	24.70382258535162
34	24.277793988911583	23.8383425736796	26.537496352494895	25.34636708491392
35	23.929967901955063	23.98365917712285	27.84768018675226	24.238692734169828
36	23.800408520571928	24.50539830755763	26.606361248905745	25.087831922964693
37	22.562007586810623	24.80653632915086	27.05923548292968	25.572220601108842
38	23.454333236066528	22.971695360373502	28.242777939889113	25.331193463670846
39	23.308433031806246	22.828713160198426	28.51590312226437	25.346950685730963
40	23.286839801575724	22.87248322147651	28.78494309892034	25.055733878027432
41	22.456959439743216	22.431281003793405	28.486139480595273	26.625620075868106
42	21.968485555879777	22.759848263787568	29.329442661219723	25.94222351911293
43	21.526116136562592	23.10767435074409	29.262328567259992	26.10388094543332
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	12.0
2	21.0
3	75.0
4	129.0
5	129.0
6	191.5
7	254.0
8	257.5
9	261.0
10	386.0
11	511.0
12	511.0
13	1098.5
14	1686.0
15	2304.0
16	2922.0
17	2620.5
18	2319.0
19	2319.0
20	2723.5
21	3128.0
22	2113.0
23	1098.0
24	1044.5
25	991.0
26	991.0
27	1057.5
28	1124.0
29	1273.5
30	1423.0
31	1602.0
32	1781.0
33	1781.0
34	1881.5
35	1982.0
36	2137.0
37	2292.0
38	3077.0
39	3862.0
40	3862.0
41	4680.5
42	5499.0
43	7154.5
44	8810.0
45	10611.0
46	12412.0
47	12412.0
48	15179.5
49	17947.0
50	17969.5
51	17992.0
52	17540.0
53	17088.0
54	17088.0
55	16280.5
56	15473.0
57	14296.0
58	13119.0
59	11791.0
60	10463.0
61	10463.0
62	9075.0
63	7687.0
64	7049.5
65	6412.0
66	5469.0
67	4526.0
68	4526.0
69	3804.0
70	3082.0
71	2583.5
72	2085.0
73	1724.0
74	1363.0
75	1363.0
76	1092.0
77	821.0
78	636.0
79	451.0
80	333.5
81	216.0
82	216.0
83	147.0
84	78.0
85	54.5
86	31.0
87	17.5
88	4.0
89	4.0
90	3.0
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	171350.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.054033699843856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.27957213745869	49.41220197951677
2	10.067947645599586	12.092417343942024
3	2.9254912719758206	5.270626543075049
4	1.3570060348010526	3.259747445793356
5	0.7809963585387021	2.3450990817569273
6	0.4659975907178906	1.679102101021095
7	0.38999797261959407	1.6394665973397522
8	0.27399857520367715	1.3163775735192662
9	0.17999906398662144	0.9728702869133622
>10	1.1729939003067902	12.858702829186134
>50	0.07399961519411695	3.2206810794378757
>100	0.02799985439777398	2.9894742519936135
>500	0.0019999895998409987	0.9632616914820723
>1k	0.0019999895998409987	1.9799711950226884
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1846	1.0773271082579516	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1451	0.8468047855266997	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	979	0.5713451998832798	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	625	0.3647505106507149	No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	481	0.2807119929967902	RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	354	0.2065946892325649	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	287	0.16749343449080828	No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC	284	0.16574263203968487	No Hit
CGCTAACCCTGTGGGAAGCTGGGGGGACCACCCTCCAAGGCTA	241	0.14064779690691567	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	235	0.1371461920046688	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	222	0.12955938138313394	No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC	208	0.12138896994455793	No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG	174	0.10154654216515903	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	5.836008170411438E-4	0.0
15	0.0	0.0	0.0	5.836008170411438E-4	0.0
16	0.0	0.0	0.0	0.0011672016340822876	0.0
17	0.0	0.0	0.0	0.0011672016340822876	0.0
18	0.0	0.0	0.0	0.0011672016340822876	0.0
19	0.0	0.0	0.0	0.0017508024511234317	0.0
20	0.0	0.0	0.0	0.0017508024511234317	0.0
21	0.0	0.0	0.0	0.0023344032681645753	0.0
22	0.0	0.0	0.0	0.0035016049022468633	0.0
23	0.0	0.0	0.0	0.005252407353370295	0.0
24	0.0	0.0	0.0	0.01050481470674059	0.0
25	0.0	0.0	0.0	0.015757222060110883	0.0
26	0.0	0.0	0.0	0.02159323023052232	0.0
27	0.0	0.0	0.0	0.0426028596440035	0.0
28	0.0	0.0	0.0	0.10096294134811788	0.0
29	0.0	0.0	0.0	0.19492267289174206	0.0
30	0.0	0.0	0.0	0.3484096877735629	0.0
31	0.0	0.0	0.0	0.648964108549752	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGCTT	30	3.5868218E-4	30.833332	37
TTCCTGT	30	3.5868218E-4	30.833332	1
TTCACGT	25	0.0054834625	29.599998	37
CTAGAAT	35	8.8385027E-4	26.42857	17
TCCTGCT	35	8.8385027E-4	26.42857	2
GCCGTCT	80	9.640644E-10	25.4375	36
TACTGGT	105	1.8189894E-12	24.666666	2
GAATCGC	40	0.0019245248	23.125	20
ACTAGAA	40	0.0019245248	23.125	16
AACTAGA	40	0.0019245248	23.125	15
ATGCCCC	40	0.0019245248	23.125	37
TATACAC	80	2.6917405E-8	23.125	3
ATTTCGG	40	0.0019245248	23.125	29
GTACTGG	105	3.8198777E-11	22.90476	1
GGTATCA	800	0.0	22.199999	1
GGTTCAC	115	1.3278623E-10	20.913042	6
ACTGGTT	115	1.3278623E-10	20.913042	3
TCTACTG	45	0.0038127354	20.555555	3
CAACTAG	45	0.0038127354	20.555555	14
AGAATCG	45	0.0038127354	20.555555	19
>>END_MODULE