FastQCFastQC Report
Fri 10 Feb 2017
ERR1632160.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632160.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393023
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27230.6928347704841701No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22680.5770654643621366No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15930.40531979044483407No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11890.3025268241298855No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.15418944947242275No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGTT358.858971E-426.4285711
CGTTTTT509.071227E-625.89999816
CATAGAC551.8985313E-523.5454542
TTGCGTT551.8985313E-523.5454544
ACGTTGC400.001928938423.12500213
GTCTATG400.001928938423.12500228
GCGTTTT652.6753914E-622.7692315
CGTCTAT502.6977205E-422.19999927
TAGCACT450.003821395320.5555574
GCATTAC450.003821395320.55555736
CTCGGTA450.003821395320.55555727
ATAGACG555.134821E-420.1818183
GAGTGTT759.240352E-619.7333347
TTTTGCG759.240352E-619.7333342
AGTGTTC759.240352E-619.7333348
TAGAGTG801.612263E-518.55
ATTAGAG902.1448905E-618.53
TGAACAG701.2166086E-418.55
TTTGCGT701.2166086E-418.53
CTCTATG2200.018.51
Produced by FastQC (version 0.11.5)