##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632160.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	393023
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85471842614809	34.0	31.0	34.0	31.0	34.0
2	33.00368680713343	34.0	33.0	34.0	31.0	34.0
3	33.05885915073672	34.0	33.0	34.0	31.0	34.0
4	36.43458016451963	37.0	37.0	37.0	35.0	37.0
5	36.325034412744294	37.0	37.0	37.0	35.0	37.0
6	36.368693435244246	37.0	37.0	37.0	35.0	37.0
7	36.34534874549327	37.0	37.0	37.0	35.0	37.0
8	36.34589578726945	37.0	37.0	37.0	35.0	37.0
9	38.140678789790925	39.0	39.0	39.0	37.0	39.0
10	38.127338603593174	39.0	38.0	39.0	37.0	39.0
11	38.18231502990919	39.0	39.0	39.0	37.0	39.0
12	38.15645394798778	39.0	39.0	39.0	37.0	39.0
13	38.171539579108604	39.0	39.0	39.0	37.0	39.0
14	39.571495815766504	41.0	39.0	41.0	37.0	41.0
15	39.59773346598036	41.0	39.0	41.0	37.0	41.0
16	39.55377166221824	41.0	39.0	41.0	37.0	41.0
17	39.56900486739962	41.0	39.0	41.0	37.0	41.0
18	39.5771010856871	41.0	39.0	41.0	37.0	41.0
19	39.613877559328586	41.0	39.0	41.0	37.0	41.0
20	39.59378967643115	41.0	39.0	41.0	37.0	41.0
21	39.55921663617651	41.0	39.0	41.0	37.0	41.0
22	39.49821511718144	41.0	39.0	41.0	37.0	41.0
23	39.43665129012806	41.0	39.0	41.0	37.0	41.0
24	39.383491551385035	41.0	39.0	41.0	36.0	41.0
25	39.327212402327596	41.0	39.0	41.0	36.0	41.0
26	39.22138399024993	40.0	39.0	41.0	36.0	41.0
27	39.11582019372912	40.0	39.0	41.0	35.0	41.0
28	39.02760652684449	40.0	39.0	41.0	35.0	41.0
29	38.95736636278284	40.0	39.0	41.0	35.0	41.0
30	38.84386409955651	40.0	38.0	41.0	35.0	41.0
31	38.72687094648405	40.0	38.0	41.0	35.0	41.0
32	38.60001323077784	40.0	38.0	41.0	35.0	41.0
33	38.47881930574037	40.0	38.0	41.0	35.0	41.0
34	38.37502130918547	40.0	38.0	41.0	35.0	41.0
35	38.30857481623213	40.0	38.0	41.0	35.0	41.0
36	38.22438127030733	40.0	38.0	41.0	34.0	41.0
37	38.11234457016511	40.0	38.0	41.0	34.0	41.0
38	38.001335799686025	40.0	37.0	41.0	34.0	41.0
39	37.883289273146865	40.0	37.0	41.0	34.0	41.0
40	37.73836391254456	40.0	37.0	41.0	33.0	41.0
41	37.6068627026917	40.0	37.0	41.0	33.0	41.0
42	37.47415800093124	40.0	37.0	41.0	33.0	41.0
43	36.575694043351156	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	1.0
13	0.0
14	2.0
15	0.0
16	0.0
17	2.0
18	5.0
19	15.0
20	33.0
21	53.0
22	106.0
23	175.0
24	305.0
25	462.0
26	790.0
27	1142.0
28	1650.0
29	2320.0
30	3052.0
31	3957.0
32	5119.0
33	6830.0
34	9779.0
35	14252.0
36	22213.0
37	43559.0
38	91545.0
39	185650.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.77326517786491	18.2961302519191	12.815789406726832	25.11481516348916
2	20.442569518832233	20.76468807169046	32.81741781015361	25.975324599323702
3	21.213007890123478	21.902026090076156	29.6038654226343	27.28110059716607
4	15.812051711986323	16.273602308261857	32.17012744801195	35.744218531739875
5	15.707223241387908	34.86157298682265	32.7581337478977	16.673070023891732
6	34.20359622719281	32.60190879414182	15.972347674309137	17.222147304356234
7	28.79475246995723	30.05930950605944	20.596758968304655	20.549179055678675
8	27.529940995819587	31.40528671350023	20.19500131035588	20.869770980324308
9	26.51829536693781	13.861020856285764	20.43646300598184	39.18422077079459
10	18.820781480982028	25.874312699256784	31.395109192082906	23.90979662767828
11	34.8519043414762	21.685499321922634	20.37285349712358	23.089742839477587
12	21.87658228653285	26.21704073298509	27.436307798780224	24.470069181701835
13	30.840179836803443	18.794319925296993	25.182241242878916	25.183258995020648
14	23.629405912631068	21.78142246128089	24.482536645438053	30.10663498064999
15	27.253621289339304	26.018579065347318	22.434564898237507	24.29323474707587
16	25.47459054559148	25.17511697788679	24.624513069209687	24.72577940731204
17	23.727110118237356	26.545520236729146	25.178424672347422	24.548944972686076
18	23.19126361561537	23.921246339272763	27.49279304264636	25.394697002465506
19	24.461163850461677	25.204377351961586	27.224106477229064	23.110352320347662
20	25.022708594662397	23.62533490406414	27.591260562358944	23.760695938914516
21	25.881945840319776	24.101388468359357	25.961076069339455	24.05558962198141
22	25.469247346847386	24.0917198230129	25.895685494233163	24.543347335906553
23	24.198329359859347	24.342341287914447	26.523129689611043	24.936199662615167
24	24.17466662256407	25.30386262381591	26.478348595374822	24.043122158245193
25	24.485844339898684	24.558868056067965	26.256478628477215	24.698808975556137
26	24.544110650012847	25.01940090020177	26.211697534240997	24.224790915544382
27	24.952738134918313	24.20138261628454	25.790348147563886	25.055531101233264
28	23.630169226737365	24.630110705989217	27.308071028921972	24.431649038351445
29	23.551293435753124	24.746388888182118	27.153372703378682	24.548944972686076
30	23.467583322095653	25.758797831170188	27.410863995236923	23.362754851497243
31	24.418163822473492	25.284270895087563	26.390821911185856	23.906743371253082
32	23.070405548784674	24.899560585512805	26.846774870681866	25.183258995020648
33	23.145719207272858	24.559376932138832	27.258710050047963	25.036193810540354
34	24.060423944654637	24.41256618569397	26.790289626815735	24.73672024283566
35	23.89834691608379	24.496021861316006	27.31494085587866	24.29069036672154
36	23.08745289715869	24.84282090361124	27.23453843668182	24.835187762548248
37	23.947453456922368	23.827358704197973	27.251076908984974	24.97411092989469
38	23.062772407721685	23.722021357528693	28.57059256074072	24.6446136740089
39	23.089233963406723	23.549003493434224	28.6474328474415	24.714329695717556
40	22.24653519005249	23.67138818847752	29.239764593929618	24.842312027540373
41	21.471771372158884	23.725074613953893	30.05829175391771	24.74486225996952
42	22.31167132712335	23.77926991550113	29.160888802945372	24.74816995443015
43	21.266185439528982	23.77952435353656	29.54738017876816	25.40691002816629
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	95.0
1	103.0
2	111.0
3	292.0
4	473.0
5	473.0
6	629.0
7	785.0
8	765.5
9	746.0
10	1080.5
11	1415.0
12	1415.0
13	2518.5
14	3622.0
15	4643.5
16	5665.0
17	4938.5
18	4212.0
19	4212.0
20	4833.0
21	5454.0
22	4232.0
23	3010.0
24	3140.0
25	3270.0
26	3270.0
27	3668.5
28	4067.0
29	5191.5
30	6316.0
31	7483.0
32	8650.0
33	8650.0
34	9898.5
35	11147.0
36	12483.0
37	13819.0
38	15559.5
39	17300.0
40	17300.0
41	18579.5
42	19859.0
43	20572.0
44	21285.0
45	23179.5
46	25074.0
47	25074.0
48	26330.0
49	27586.0
50	29041.5
51	30497.0
52	31780.0
53	33063.0
54	33063.0
55	30677.0
56	28291.0
57	27537.5
58	26784.0
59	24371.5
60	21959.0
61	21959.0
62	20473.5
63	18988.0
64	16960.0
65	14932.0
66	13120.5
67	11309.0
68	11309.0
69	9612.5
70	7916.0
71	7028.5
72	6141.0
73	4830.0
74	3519.0
75	3519.0
76	2759.0
77	1999.0
78	1688.5
79	1378.0
80	1109.5
81	841.0
82	841.0
83	680.5
84	520.0
85	465.0
86	410.0
87	368.0
88	326.0
89	326.0
90	248.5
91	171.0
92	90.5
93	10.0
94	8.0
95	6.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	393023.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.37908749027981
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.60106258247446	48.824888840885244
2	7.199997733760102	8.118586043229543
3	1.9884990143370922	3.3632927971093824
4	0.9464454369650125	2.134389203817062
5	0.5677765396582057	1.6005361602159152
6	0.3713357787403182	1.2561343414722952
7	0.27574139468536524	1.0882233750960588
8	0.21807308678043857	0.9835809311095782
9	0.17195232655349016	0.8725063736624745
>10	1.3961956348109263	16.16266684323111
>50	0.18883291576648464	7.316097711375462
>100	0.07182878853316063	6.14501268867924
>500	4.5175338700101023E-4	0.15434482900234675
>1k	0.001807013548004041	1.9797398611142596
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2723	0.6928347704841701	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2268	0.5770654643621366	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1593	0.40531979044483407	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1189	0.3025268241298855	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	606	0.15418944947242275	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.5443803543304084E-4	0.0
5	0.0	0.0	0.0	2.5443803543304084E-4	0.0
6	0.0	0.0	0.0	2.5443803543304084E-4	0.0
7	0.0	0.0	0.0	2.5443803543304084E-4	0.0
8	0.0	0.0	0.0	5.088760708660817E-4	0.0
9	0.0	0.0	0.0	5.088760708660817E-4	0.0
10	0.0	0.0	0.0	5.088760708660817E-4	0.0
11	0.0	0.0	0.0	5.088760708660817E-4	0.0
12	0.0	0.0	0.0	5.088760708660817E-4	0.0
13	0.0	0.0	0.0	7.633141062991225E-4	0.0
14	0.0	0.0	0.0	7.633141062991225E-4	0.0
15	0.0	0.0	0.0	7.633141062991225E-4	0.0
16	0.0	0.0	0.0	0.0017810662480312856	0.0
17	0.0	0.0	0.0	0.00305325642519649	0.0
18	0.0	0.0	0.0	0.0033076944606295305	0.0
19	0.0	0.0	0.0	0.004834322673227776	0.0
20	0.0	0.0	0.0	0.005343198744093857	0.0
21	0.0	0.0	0.0	0.00610651285039298	0.0
22	0.0	0.0	0.0	0.008396455169290346	0.0
23	0.0	0.0	0.0	0.01221302570078596	0.0
24	0.0	0.0	0.0	0.022136109082674552	0.0
25	0.0	0.0	0.0	0.02798818389763449	0.0
26	0.0	0.0	0.0	0.03714795317322396	0.0
27	0.0	0.0	0.0	0.07836691491337656	0.0
28	0.0	0.0	0.0	0.17912437694486075	0.0
29	0.0	0.0	0.0	0.299473567704689	0.0
30	0.0	0.0	0.0	0.4763080023306524	0.0
31	0.0	0.0	0.0	0.8528762947715528	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACGTT	35	8.858971E-4	26.42857	11
CGTTTTT	50	9.071227E-6	25.899998	16
CATAGAC	55	1.8985313E-5	23.545454	2
TTGCGTT	55	1.8985313E-5	23.545454	4
ACGTTGC	40	0.0019289384	23.125002	13
GTCTATG	40	0.0019289384	23.125002	28
GCGTTTT	65	2.6753914E-6	22.76923	15
CGTCTAT	50	2.6977205E-4	22.199999	27
TAGCACT	45	0.0038213953	20.555557	4
GCATTAC	45	0.0038213953	20.555557	36
CTCGGTA	45	0.0038213953	20.555557	27
ATAGACG	55	5.134821E-4	20.181818	3
GAGTGTT	75	9.240352E-6	19.733334	7
TTTTGCG	75	9.240352E-6	19.733334	2
AGTGTTC	75	9.240352E-6	19.733334	8
TAGAGTG	80	1.612263E-5	18.5	5
ATTAGAG	90	2.1448905E-6	18.5	3
TGAACAG	70	1.2166086E-4	18.5	5
TTTGCGT	70	1.2166086E-4	18.5	3
CTCTATG	220	0.0	18.5	1
>>END_MODULE