FastQCFastQC Report
Fri 10 Feb 2017
ERR1632159.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632159.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318427
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4200.13189836289008156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.10928721496606757No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG3390.10646082147556582No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA3340.10489060286973152No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG3300.10363442798506407No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC3260.10237825310039662No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA3240.10175016565806291No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA250.005490449729.63
AGCGTAT250.005490449729.614
ACGTTCG250.005490449729.614
TATTACT250.005490449729.64
GCGTATA250.005490449729.615
GACGTTC400.001928138423.12500213
ATTACTG400.001928138423.1250025
TGGGTCG400.001928138423.12500223
GGTATCA2900.022.9655171
ACAACAC603.715406E-521.5833323
GGGTCGT450.003819825820.55555724
CAGACGT450.003819825820.55555711
CGCGGTT759.2313185E-619.73333210
TTTCGGA759.2313185E-619.73333230
TCAGGAC801.6106962E-518.53
GGTATCC500.007023987418.526
GTATCCA500.007023987418.527
GTTTTCG801.6106962E-518.528
TGTAGTT500.007023987418.512
CAAGACG701.21568766E-418.54
Produced by FastQC (version 0.11.5)