##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632159.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	318427
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8151852700933	34.0	31.0	34.0	31.0	34.0
2	32.98111027017181	34.0	33.0	34.0	31.0	34.0
3	33.056581257242634	34.0	33.0	34.0	31.0	34.0
4	36.43157458381356	37.0	37.0	37.0	35.0	37.0
5	36.30813027789729	37.0	37.0	37.0	35.0	37.0
6	36.34009050740044	37.0	37.0	37.0	35.0	37.0
7	36.32135779943284	37.0	37.0	37.0	35.0	37.0
8	36.30508405380197	37.0	37.0	37.0	35.0	37.0
9	38.1059583515217	39.0	39.0	39.0	37.0	39.0
10	38.08710316650284	39.0	38.0	39.0	37.0	39.0
11	38.1464354467429	39.0	39.0	39.0	37.0	39.0
12	38.122320029394494	39.0	38.0	39.0	37.0	39.0
13	38.1275174529798	39.0	39.0	39.0	37.0	39.0
14	39.53645262493444	41.0	39.0	41.0	37.0	41.0
15	39.56149447126029	41.0	39.0	41.0	37.0	41.0
16	39.54290622340442	41.0	39.0	41.0	37.0	41.0
17	39.545566173722705	41.0	39.0	41.0	37.0	41.0
18	39.53628618176222	41.0	39.0	41.0	37.0	41.0
19	39.572457737566225	41.0	39.0	41.0	37.0	41.0
20	39.55771024442023	41.0	39.0	41.0	37.0	41.0
21	39.529688123180506	41.0	39.0	41.0	37.0	41.0
22	39.47838594089069	41.0	39.0	41.0	37.0	41.0
23	39.439212127112334	41.0	39.0	41.0	37.0	41.0
24	39.390425435029066	41.0	39.0	41.0	37.0	41.0
25	39.34857910918358	40.0	39.0	41.0	36.0	41.0
26	39.28590854418752	40.0	39.0	41.0	36.0	41.0
27	39.206976795309444	40.0	39.0	41.0	36.0	41.0
28	39.14063505921295	40.0	39.0	41.0	36.0	41.0
29	39.0988012951163	40.0	39.0	41.0	36.0	41.0
30	39.03189113988449	40.0	39.0	41.0	35.0	41.0
31	38.970395098405604	40.0	39.0	41.0	35.0	41.0
32	38.88472083083407	40.0	38.0	41.0	35.0	41.0
33	38.82229521994052	40.0	38.0	41.0	35.0	41.0
34	38.77059106168761	40.0	38.0	41.0	35.0	41.0
35	38.74211985792662	40.0	38.0	41.0	35.0	41.0
36	38.70457907149834	40.0	38.0	41.0	35.0	41.0
37	38.65404001545095	40.0	38.0	41.0	35.0	41.0
38	38.60242064900275	40.0	38.0	41.0	35.0	41.0
39	38.55800544551813	40.0	38.0	41.0	35.0	41.0
40	38.493171119283225	40.0	38.0	41.0	35.0	41.0
41	38.45529430607329	40.0	38.0	41.0	35.0	41.0
42	38.41230172064555	40.0	38.0	41.0	34.0	41.0
43	37.565671252751805	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	5.0
18	9.0
19	12.0
20	20.0
21	37.0
22	77.0
23	104.0
24	205.0
25	277.0
26	431.0
27	652.0
28	932.0
29	1336.0
30	1897.0
31	2524.0
32	3439.0
33	4835.0
34	6903.0
35	10237.0
36	16664.0
37	31509.0
38	78309.0
39	158012.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.314250990022835	18.317856211941827	12.937973224632332	26.42991957340301
2	19.870802413111953	20.619796688094915	33.91829210462681	25.591108794166324
3	20.960220081839793	21.373501618895382	29.382244596092665	28.284033703172156
4	15.848216388685632	15.89155442220666	32.57732541524431	35.682903773863394
5	16.27154732481856	34.62269217120407	33.03771351047493	16.068046993502435
6	35.70582896550858	32.69226541719138	15.51030534471009	16.09160027258995
7	29.656090720950175	29.04401950839596	20.57237608619872	20.72751368445515
8	26.677700069403663	32.08113633580067	20.583681660160728	20.657481934634937
9	26.331937932398947	14.37001259315322	20.04007197882089	39.25797749562694
10	17.740643852437135	26.075992299647954	32.70702547208623	23.476338375828686
11	35.84934694608183	21.185075386195265	20.666903246269946	22.298674421452954
12	22.160809227860703	25.118787037531366	27.764291344641002	24.95611238996693
13	30.727607897571502	18.930869555659537	24.398684784895753	25.942837761873207
14	23.847538054247913	21.408988559387236	23.299217717090574	31.444255669274273
15	26.666394495441654	26.913546903999976	21.532093698084648	24.887964902473723
16	26.406052250594325	25.515738301086277	22.97983525266388	25.09837419565552
17	24.672844953474424	25.55279546018397	24.273695383871345	25.50066420247027
18	23.813307288640726	24.180738442405953	26.449390284115353	25.55656398483797
19	25.21739676597776	25.009185778844127	25.35274960980068	24.420667845377434
20	25.801832131069286	24.08997980698873	25.679983167256548	24.428204894685436
21	26.138172956438993	24.815420802884177	23.983204941791996	25.06320129888483
22	25.69662748447839	24.223448388484645	24.331479428566045	25.748444698470923
23	24.898642388993395	24.44893178028245	24.72403408002462	25.928391750699532
24	25.723007157056404	24.93538550436992	24.699224626052438	24.64238271252124
25	25.705106664949895	24.49509620729398	24.38486686116441	25.414930266591718
26	25.517622563413276	24.623540089251225	24.61506090877972	25.243776438555777
27	25.870921749725994	23.80294384584222	24.44013855608978	25.88599584834201
28	24.56795435060469	24.349693964393722	25.240636001344107	25.84171568365748
29	24.222506257321143	24.606581728308218	25.445078463823734	25.725833550546906
30	24.191101885204457	25.956341641883384	25.38415398191736	24.468402490994794
31	25.839203333888143	24.767372113545647	24.570780744095195	24.822643808471014
32	24.02685701903419	24.46777440355246	25.236867476690104	26.268501100723242
33	24.288769482487353	24.089351719546396	25.48747436618126	26.13440443178499
34	25.117530862646696	23.825240950045064	25.32040310652049	25.736825080787746
35	24.918427143426907	23.971899367829987	25.644810270485856	25.464863218257243
36	24.36288380068273	24.549739814777013	25.828211803647307	25.25916458089295
37	25.42560775311139	23.106708916015286	25.642926008158856	25.82475732271447
38	24.070195052555217	23.216938262144858	27.213144613993162	25.499722071306767
39	24.189531666598622	22.564669453281287	27.7065072999463	25.53929158017379
40	23.316176078033585	22.412358248515357	28.476228460526276	25.795237212924782
41	22.540802130472603	22.821871260916947	29.317237545811125	25.320089062799322
42	23.378042691103456	22.485530435547236	28.566987095943496	25.56943977740581
43	22.104281358050667	22.49715005323041	29.184083008036378	26.214485580682545
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	9.0
2	13.0
3	34.5
4	56.0
5	56.0
6	88.0
7	120.0
8	102.0
9	84.0
10	132.0
11	180.0
12	180.0
13	337.5
14	495.0
15	737.5
16	980.0
17	949.5
18	919.0
19	919.0
20	1132.0
21	1345.0
22	1465.5
23	1586.0
24	1897.0
25	2208.0
26	2208.0
27	2673.5
28	3139.0
29	4371.5
30	5604.0
31	6425.0
32	7246.0
33	7246.0
34	8248.5
35	9251.0
36	10328.5
37	11406.0
38	13499.0
39	15592.0
40	15592.0
41	16779.0
42	17966.0
43	17806.5
44	17647.0
45	19330.0
46	21013.0
47	21013.0
48	22480.0
49	23947.0
50	25818.5
51	27690.0
52	29887.0
53	32084.0
54	32084.0
55	29064.5
56	26045.0
57	24754.5
58	23464.0
59	21042.0
60	18620.0
61	18620.0
62	17042.0
63	15464.0
64	13490.0
65	11516.0
66	9784.5
67	8053.0
68	8053.0
69	6703.0
70	5353.0
71	4645.0
72	3937.0
73	2997.5
74	2058.0
75	2058.0
76	1615.5
77	1173.0
78	991.5
79	810.0
80	682.0
81	554.0
82	554.0
83	417.5
84	281.0
85	262.5
86	244.0
87	203.5
88	163.0
89	163.0
90	128.5
91	94.0
92	54.5
93	15.0
94	10.0
95	5.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	318427.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.96825255083594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.44232876925996	56.66521323010072
2	4.362838154911987	5.407149132440182
3	1.0952015804043143	2.036031843857076
4	0.5915503335215642	1.4662936185678208
5	0.39797122600854623	1.2330790720631704
6	0.24902098726309146	0.925883725750665
7	0.20246954500326506	0.878267872903062
8	0.15654966557061792	0.7760887370263166
9	0.13120814429667718	0.7317665480252714
>10	1.1163192614032877	15.058122719325976
>50	0.1840614873415627	7.909395177234026
>100	0.07048084501508872	6.9127083227057255
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	420	0.13189836289008156	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	348	0.10928721496606757	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	339	0.10646082147556582	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	334	0.10489060286973152	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	330	0.10363442798506407	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	326	0.10237825310039662	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	324	0.10175016565806291	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	3.1404372116686084E-4	0.0	0.0	0.0
14	0.0	3.1404372116686084E-4	0.0	0.0	0.0
15	0.0	3.1404372116686084E-4	0.0	0.0	0.0
16	0.0	3.1404372116686084E-4	0.0	6.280874423337217E-4	0.0
17	0.0	3.1404372116686084E-4	0.0	0.0012561748846674433	0.0
18	0.0	3.1404372116686084E-4	0.0	0.0012561748846674433	0.0
19	0.0	3.1404372116686084E-4	0.0	0.0012561748846674433	0.0
20	0.0	3.1404372116686084E-4	0.0	0.0012561748846674433	0.0
21	0.0	3.1404372116686084E-4	0.0	0.0018842623270011651	0.0
22	3.1404372116686084E-4	3.1404372116686084E-4	0.0	0.0028263934905017477	0.0
23	3.1404372116686084E-4	3.1404372116686084E-4	0.0	0.0031404372116686087	0.0
24	3.1404372116686084E-4	3.1404372116686084E-4	0.0	0.005966830702170356	0.0
25	3.1404372116686084E-4	3.1404372116686084E-4	0.0	0.008165136750338382	0.0
26	3.1404372116686084E-4	3.1404372116686084E-4	0.0	0.016330273500676763	0.0
27	3.1404372116686084E-4	3.1404372116686084E-4	0.0	0.036115027934189	0.0
28	3.1404372116686084E-4	3.1404372116686084E-4	0.0	0.08856032936905477	0.0
29	3.1404372116686084E-4	3.1404372116686084E-4	0.0	0.15764994802576415	0.0
30	3.1404372116686084E-4	3.1404372116686084E-4	0.0	0.27384612485750265	0.0
31	3.1404372116686084E-4	3.1404372116686084E-4	0.0	0.5577416487923449	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTCA	25	0.0054904497	29.6	3
AGCGTAT	25	0.0054904497	29.6	14
ACGTTCG	25	0.0054904497	29.6	14
TATTACT	25	0.0054904497	29.6	4
GCGTATA	25	0.0054904497	29.6	15
GACGTTC	40	0.0019281384	23.125002	13
ATTACTG	40	0.0019281384	23.125002	5
TGGGTCG	40	0.0019281384	23.125002	23
GGTATCA	290	0.0	22.965517	1
ACAACAC	60	3.715406E-5	21.583332	3
GGGTCGT	45	0.0038198258	20.555557	24
CAGACGT	45	0.0038198258	20.555557	11
CGCGGTT	75	9.2313185E-6	19.733332	10
TTTCGGA	75	9.2313185E-6	19.733332	30
TCAGGAC	80	1.6106962E-5	18.5	3
GGTATCC	50	0.0070239874	18.5	26
GTATCCA	50	0.0070239874	18.5	27
GTTTTCG	80	1.6106962E-5	18.5	28
TGTAGTT	50	0.0070239874	18.5	12
CAAGACG	70	1.21568766E-4	18.5	4
>>END_MODULE