FastQCFastQC Report
Fri 10 Feb 2017
ERR1632158.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632158.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98157
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14111.437492995914708No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12631.2867141416302454No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8660.882260052772599No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT6670.6795236203225444RNA PCR Primer, Index 26 (100% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT5060.5155006774860683No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4300.43807369825891174No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3420.3484214065222042No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC2950.3005389325264627No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC2490.2536752345731838No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG2310.23533726580885722No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG2000.20375520849251708No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC1940.19764255223774155No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1690.17217315117617696No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1640.16707927096386402No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1600.16300416679401367No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1590.1619853907515511No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1580.16096661470908852No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA1560.15892906262416334No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.154853958454313No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC1490.15179763032692523RNA PCR Primer, Index 26 (100% over 23bp)
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA1450.1477225261570749No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT1450.1477225261570749No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTC1370.1395723178173742No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC1320.13447843760506129No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.1334596615625987No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT1280.13040333343521093No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGA1240.12632822926536058No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT1240.12632822926536058No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAA1180.1202155730105851No Hit
CTAATAGGGCACACCTTCTTCCGAAGTTACGGTGTCAATTTGC1170.1191967969681225No Hit
GTGCTGTGTTTTTAATAAACAGTCGCAGCCACCGATTTTTTGC1160.11817802092565992No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCG1140.11614046884073474No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG1140.11614046884073474No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG1140.11614046884073474No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT1110.11308414071334698No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT1090.1110465886284218No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAG1060.10799026050103407No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGA1000.10187760424625854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTGA250.005472188329.5999981
TATACAC706.4355845E-926.428573
CACTGTT508.980991E-625.8999982
CCACTGT601.3167064E-624.6666681
ATACACA751.3415047E-824.6666664
CCTATGG400.001918698123.1250021
TACACAT802.662091E-823.1250025
ACTGTTC502.6777838E-422.1999993
GCCTTAT2550.021.76470626
TTATACA855.0600647E-821.7647062
CAGTCGG3300.021.3030310
GTGATTC3250.021.06153916
GCAGTCG3350.020.9850759
TCGCCTT2650.020.94339624
GGTGATT3300.020.74242415
GATTCCT3300.020.74242418
CCTTATT2700.020.55555527
TCTTATA2250.020.55555537
GGCAGTC3450.020.3768128
TCGGTGA3450.020.37681213
Produced by FastQC (version 0.11.5)