FastQCFastQC Report
Fri 10 Feb 2017
ERR1632157.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632157.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77755
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT900.11574818339656615TruSeq Adapter, Index 12 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT1251.8189894E-1120.7237
GAGAAAA500.006977487418.521
TCTGGAG500.006977487418.53
TCTCTTA2055.456968E-1215.34146337
GTCTCTT2602.8558134E-1012.09615436
GCTGTCT1553.5155585E-410.74193537
CCTGTCT2751.03431375E-710.09090935
TGTCTCT3605.820766E-109.76388837
CTGTCTC4604.7166395E-87.641303536
Produced by FastQC (version 0.11.5)