##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632157.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	77755
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.79522860266221	31.0	30.0	33.0	28.0	34.0
2	30.97552568966626	31.0	31.0	34.0	27.0	34.0
3	31.070567809144105	31.0	31.0	34.0	27.0	34.0
4	34.88818725483892	35.0	35.0	37.0	32.0	37.0
5	33.53803613915504	35.0	33.0	37.0	28.0	37.0
6	33.44403575332776	35.0	33.0	37.0	28.0	37.0
7	33.4438299787795	35.0	33.0	37.0	28.0	37.0
8	33.358755063983025	35.0	33.0	37.0	28.0	37.0
9	34.84069191691852	37.0	33.0	39.0	29.0	39.0
10	34.44862709793583	37.0	33.0	39.0	27.0	39.0
11	34.651816603433865	37.0	33.0	39.0	27.0	39.0
12	34.56404089769147	37.0	33.0	39.0	27.0	39.0
13	34.702938717767346	37.0	33.0	39.0	27.0	39.0
14	35.52909780721497	38.0	33.0	39.0	28.0	40.0
15	35.42979872676998	38.0	33.0	39.0	27.0	40.0
16	35.38077294064691	38.0	33.0	39.0	27.0	40.0
17	35.336081280946566	38.0	33.0	39.0	27.0	40.0
18	35.289331875763615	37.0	33.0	39.0	27.0	40.0
19	35.32383769532506	38.0	33.0	40.0	27.0	40.0
20	35.28020063018455	37.0	33.0	39.0	27.0	40.0
21	35.24437013696868	37.0	33.0	39.0	27.0	40.0
22	35.14363063468587	37.0	33.0	39.0	27.0	40.0
23	35.03192077679892	37.0	33.0	39.0	27.0	40.0
24	34.93458941547168	37.0	33.0	39.0	27.0	40.0
25	34.800630184554045	37.0	33.0	39.0	26.0	40.0
26	34.59539579448267	37.0	33.0	39.0	26.0	40.0
27	34.445540479711916	37.0	32.0	39.0	25.0	40.0
28	34.31546524339271	37.0	32.0	39.0	25.0	40.0
29	34.17971834608707	37.0	32.0	39.0	25.0	40.0
30	34.02734229310012	37.0	32.0	39.0	24.0	40.0
31	33.89466915310913	37.0	31.0	39.0	24.0	40.0
32	33.722680213491095	36.0	31.0	39.0	24.0	40.0
33	33.55970677126873	36.0	31.0	39.0	24.0	40.0
34	33.46487042633914	36.0	31.0	39.0	23.0	40.0
35	33.4102244228667	36.0	31.0	39.0	23.0	40.0
36	33.318076007973765	36.0	31.0	39.0	23.0	40.0
37	33.22133624847277	36.0	31.0	39.0	23.0	40.0
38	33.12496945534049	36.0	30.0	38.0	23.0	40.0
39	33.07292135553983	36.0	30.0	38.0	23.0	40.0
40	32.97246479326088	35.0	30.0	38.0	22.0	40.0
41	32.85643366986046	35.0	30.0	38.0	22.0	40.0
42	32.785029901614045	35.0	30.0	38.0	22.0	40.0
43	31.749302295672305	34.0	29.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	0.0
15	6.0
16	12.0
17	30.0
18	51.0
19	74.0
20	118.0
21	208.0
22	335.0
23	473.0
24	733.0
25	1027.0
26	1358.0
27	1832.0
28	2484.0
29	3117.0
30	3899.0
31	4788.0
32	5759.0
33	6902.0
34	7992.0
35	8859.0
36	9509.0
37	8898.0
38	6764.0
39	2525.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.945855571988936	19.01742653205582	12.57282489872034	26.463892997234908
2	21.44299401967719	21.24622210790303	31.20956851649412	26.101215355925667
3	22.348402032023664	21.890553662143912	28.020063018455403	27.74098128737702
4	17.654170149829593	15.906372580541445	31.61082888560221	34.82862838402675
5	17.351938782071894	33.94508391743296	31.936209890039223	16.76676741045592
6	37.07414314192013	32.24744389428333	14.363063468587228	16.31534949520931
7	30.64626069063083	28.135811201851972	19.831522088611663	21.38640601890554
8	28.449617387949328	31.131116969969774	19.009709986496045	21.40955565558485
9	27.316571281589606	13.58240627612372	18.54671725290978	40.55430518937689
10	18.517137161597326	25.312841617902386	31.734293614558553	24.43572760594174
11	38.14674297472831	19.909973635136005	19.79293936081281	22.150344029322873
12	22.97601440421838	23.665359140891262	27.279274644717383	26.07935181017298
13	32.43264098771783	18.40653334190727	22.381840396116008	26.778985274258886
14	24.85113497524275	19.638608449617386	22.492444215806056	33.0178123593338
15	27.501768375024117	26.07034917368658	20.014147000192914	26.413735451096393
16	27.622660922127196	24.285254967526203	22.170921484148927	25.921162626197674
17	25.684521895698026	24.79197479261784	23.195935952671853	26.32756735901228
18	26.089640537586007	23.266670953636424	24.273680149186546	26.370008359591026
19	27.03620345958459	24.01388978200759	23.823548324866568	25.126358433541252
20	26.74426081923992	23.171500225065913	23.76052986946177	26.323709086232395
21	27.266413735451096	23.30268149958202	23.246093498810367	26.184811266156515
22	27.483763102051316	23.43129059224487	23.189505498038713	25.895440807665103
23	26.807279274644717	23.36312777313356	22.89499067584078	26.934602276380943
24	26.69024500032152	23.783679506141084	22.995305768117806	26.53076972541959
25	26.973185004179793	23.38113304610636	23.144492315606712	26.50118963410713
26	27.014339913831908	23.880136325638222	22.74837631020513	26.357147450324735
27	26.79570445630506	23.112340042441	23.08661822390843	27.00533727734551
28	26.184811266156515	23.84026750691274	23.239663044177224	26.73525818275352
29	26.043341264227383	23.796540415407367	23.868561507298566	26.291556813066684
30	26.44074336055559	23.742524596488973	23.809401324673654	26.007330718281786
31	26.6079351810173	23.38884959166613	23.392707864446017	26.610507362870557
32	26.45746254260176	23.409427046492187	23.357983409427046	26.775127001479003
33	26.180952993376632	23.350266863867276	23.797826506333998	26.670953636422094
34	26.93588836730757	23.430004501318244	23.1213426789274	26.512764452446785
35	26.21696353932223	22.928429039933125	24.392000514436372	26.462606906308277
36	26.425310269436046	23.255096135296764	23.530319593595266	26.78927400167192
37	26.36486399588451	22.493730306732687	24.605491608256706	26.535914089126102
38	25.51604398430969	22.519452125265257	25.068484341842968	26.896019548582085
39	25.208668252845477	22.37026557777635	25.706385441450713	26.714680727927465
40	25.17137161597325	22.107903028744133	26.224680084882003	26.49604527040062
41	24.569481062311105	22.079609028358306	26.65552054530255	26.69538936402804
42	24.686515336634297	22.08346730113819	26.46646517908816	26.76355218313935
43	23.975307054208734	21.96386084496174	26.82399845669089	27.236833644138642
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	3.0
2	3.0
3	6.0
4	9.0
5	9.0
6	11.0
7	13.0
8	10.5
9	8.0
10	11.0
11	14.0
12	14.0
13	31.0
14	48.0
15	70.0
16	92.0
17	86.5
18	81.0
19	81.0
20	151.5
21	222.0
22	241.5
23	261.0
24	333.5
25	406.0
26	406.0
27	460.0
28	514.0
29	715.0
30	916.0
31	1153.5
32	1391.0
33	1391.0
34	1690.0
35	1989.0
36	2226.0
37	2463.0
38	2806.5
39	3150.0
40	3150.0
41	3486.5
42	3823.0
43	4019.0
44	4215.0
45	4502.0
46	4789.0
47	4789.0
48	5094.5
49	5400.0
50	5685.5
51	5971.0
52	6256.5
53	6542.0
54	6542.0
55	6445.5
56	6349.0
57	6138.5
58	5928.0
59	5635.0
60	5342.0
61	5342.0
62	5075.5
63	4809.0
64	4346.0
65	3883.0
66	3392.0
67	2901.0
68	2901.0
69	2526.5
70	2152.0
71	1853.5
72	1555.0
73	1290.0
74	1025.0
75	1025.0
76	814.0
77	603.0
78	510.5
79	418.0
80	322.5
81	227.0
82	227.0
83	178.0
84	129.0
85	95.5
86	62.0
87	46.5
88	31.0
89	31.0
90	21.5
91	12.0
92	8.5
93	5.0
94	3.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	77755.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.7505626647804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.30500811500116	83.69622532313035
2	3.5256781822397403	6.258118448974343
3	0.9042429863204267	2.4075622146485753
4	0.4245884535126362	1.507298566008617
5	0.26663575237653603	1.1832036524982317
6	0.15215627173661025	0.8102372837759629
7	0.0941919777417111	0.5851713716159733
8	0.0883955483422212	0.6276123721947141
9	0.05506607929515419	0.4398430969069513
>10	0.1811384187340598	2.2892418493987527
>50	0.002898214699744957	0.19548582084753394
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	90	0.11574818339656615	TruSeq Adapter, Index 12 (95% over 22bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0012860909266285126	0.0	0.0	0.0
3	0.0	0.0012860909266285126	0.0	0.0	0.0
4	0.0	0.0012860909266285126	0.0	0.0	0.0
5	0.0	0.0012860909266285126	0.0	0.0	0.0
6	0.0	0.0012860909266285126	0.0	0.0	0.0
7	0.0	0.0012860909266285126	0.0	0.0	0.0
8	0.0	0.0012860909266285126	0.0	0.0	0.0
9	0.0	0.0012860909266285126	0.0	0.0	0.0
10	0.0	0.0012860909266285126	0.0	0.0	0.0
11	0.0	0.0012860909266285126	0.0	0.0	0.0
12	0.0	0.0012860909266285126	0.0	0.0	0.0
13	0.0	0.0012860909266285126	0.0	0.0	0.0
14	0.0	0.0012860909266285126	0.0	0.0	0.0
15	0.0	0.0012860909266285126	0.0	0.0	0.0
16	0.0	0.0012860909266285126	0.0	0.0	0.0
17	0.0	0.0012860909266285126	0.0	0.0	0.0
18	0.0	0.002572181853257025	0.0	0.0	0.0
19	0.0	0.002572181853257025	0.0	0.0	0.0
20	0.0	0.002572181853257025	0.0	0.0	0.0
21	0.0	0.002572181853257025	0.0	0.0	0.0
22	0.0	0.002572181853257025	0.0	0.0	0.0
23	0.0	0.002572181853257025	0.0	0.0012860909266285126	0.0
24	0.0	0.002572181853257025	0.0	0.0012860909266285126	0.0
25	0.0	0.002572181853257025	0.0	0.0012860909266285126	0.0
26	0.0	0.002572181853257025	0.0	0.003858272779885538	0.0
27	0.0	0.002572181853257025	0.0	0.0205774548260562	0.0
28	0.0	0.002572181853257025	0.0	0.055301909845026045	0.0
29	0.0	0.002572181853257025	0.0	0.12603691080959423	0.0
30	0.0012860909266285126	0.002572181853257025	0.0	0.1929136389942769	0.0
31	0.0012860909266285126	0.002572181853257025	0.0	0.3395280046299273	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCTTAT	125	1.8189894E-11	20.72	37
GAGAAAA	50	0.0069774874	18.5	21
TCTGGAG	50	0.0069774874	18.5	3
TCTCTTA	205	5.456968E-12	15.341463	37
GTCTCTT	260	2.8558134E-10	12.096154	36
GCTGTCT	155	3.5155585E-4	10.741935	37
CCTGTCT	275	1.03431375E-7	10.090909	35
TGTCTCT	360	5.820766E-10	9.763888	37
CTGTCTC	460	4.7166395E-8	7.6413035	36
>>END_MODULE