FastQCFastQC Report
Fri 10 Feb 2017
ERR1632156.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632156.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69441
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT1040.14976742846445182RNA PCR Primer, Index 17 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC652.8885552E-928.46153837
GTATCAA450.003790239620.5555571
ATCAACG500.006970106618.4999983
TCTTATA1301.3285899E-817.07692337
CTCTTAT2250.016.44444537
GTCTCTT4750.012.46315837
TCTCTTA3051.8189894E-1212.13114736
GAGTACA1150.005134998411.260876
AGAGTAC1150.005134998411.260875
CAGAGTA1150.005134998411.260874
AGTACAT1150.005134998411.260877
TGTCTCT6450.09.17829536
CTGTCTC8050.07.58385137
CCTGTCT4351.2336586E-67.22988537
GCTGTCT3257.5472053E-46.83076935
Produced by FastQC (version 0.11.5)