##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632156.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	69441
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63730361025907	34.0	31.0	34.0	31.0	34.0
2	32.81340994513328	34.0	31.0	34.0	31.0	34.0
3	32.894183551504156	34.0	31.0	34.0	31.0	34.0
4	36.32758744833744	37.0	37.0	37.0	35.0	37.0
5	36.18467475986809	37.0	35.0	37.0	35.0	37.0
6	36.204029319854264	37.0	36.0	37.0	35.0	37.0
7	36.19677135985945	37.0	36.0	37.0	35.0	37.0
8	36.17708558344494	37.0	36.0	37.0	35.0	37.0
9	37.97440993073256	39.0	38.0	39.0	35.0	39.0
10	37.91494938148932	39.0	38.0	39.0	35.0	39.0
11	37.9882202157227	39.0	38.0	39.0	35.0	39.0
12	37.936996875045004	39.0	38.0	39.0	35.0	39.0
13	37.966028715024265	39.0	38.0	39.0	35.0	39.0
14	39.323195230483435	40.0	39.0	41.0	36.0	41.0
15	39.33257009547673	40.0	39.0	41.0	37.0	41.0
16	39.32650739476678	40.0	39.0	41.0	36.0	41.0
17	39.30454630549675	40.0	39.0	41.0	36.0	41.0
18	39.29970766550021	40.0	39.0	41.0	36.0	41.0
19	39.31838539191544	40.0	39.0	41.0	36.0	41.0
20	39.292406503362564	40.0	39.0	41.0	36.0	41.0
21	39.26225140766982	40.0	39.0	41.0	36.0	41.0
22	39.22316786912631	40.0	39.0	41.0	36.0	41.0
23	39.17610633487421	40.0	39.0	41.0	36.0	41.0
24	39.143013493469276	40.0	39.0	41.0	36.0	41.0
25	39.067193732809145	40.0	39.0	41.0	36.0	41.0
26	38.97857173715816	40.0	38.0	41.0	35.0	41.0
27	38.88771763079449	40.0	38.0	41.0	35.0	41.0
28	38.83106522083495	40.0	38.0	41.0	35.0	41.0
29	38.76584438588154	40.0	38.0	41.0	35.0	41.0
30	38.702870062355096	40.0	38.0	41.0	35.0	41.0
31	38.632882590976514	40.0	38.0	41.0	35.0	41.0
32	38.53914834175775	40.0	38.0	41.0	35.0	41.0
33	38.46383260609726	40.0	38.0	41.0	34.0	41.0
34	38.39080658400657	40.0	38.0	41.0	34.0	41.0
35	38.34776284903731	40.0	38.0	41.0	34.0	41.0
36	38.31435319191832	40.0	38.0	41.0	34.0	41.0
37	38.25410060338993	40.0	38.0	41.0	34.0	41.0
38	38.229144165550615	40.0	38.0	41.0	34.0	41.0
39	38.1648593770251	40.0	38.0	41.0	34.0	41.0
40	38.0882619777941	40.0	37.0	41.0	34.0	41.0
41	38.05446350138967	40.0	37.0	41.0	34.0	41.0
42	37.98004061001426	40.0	37.0	41.0	33.0	41.0
43	37.0682161835227	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	2.0
20	8.0
21	10.0
22	14.0
23	24.0
24	45.0
25	79.0
26	117.0
27	168.0
28	246.0
29	326.0
30	518.0
31	673.0
32	1015.0
33	1296.0
34	1850.0
35	2862.0
36	4653.0
37	8111.0
38	18989.0
39	28434.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.02213389784133	17.241975201970018	11.956913062887919	27.77897783730073
2	20.76006969945709	18.743969700897164	32.13951411990035	28.356446479745394
3	21.398021341858556	20.391411414006136	27.50680433749514	30.70376290664017
4	16.57090191673507	14.321510346913207	31.69165190593454	37.41593583041719
5	17.7359197016172	33.42693797612362	32.07039069137829	16.76675163088089
6	38.11005025849282	32.17551590558892	13.994614132860988	15.719819703057272
7	32.08911161993635	27.257671980530233	19.445284486110513	21.207931913422907
8	29.344335479039763	30.71960369234314	18.131939344191473	21.80412148442563
9	27.668092337379935	13.152172347748447	17.887127201509195	41.29260811336242
10	19.139989343471438	24.144237554182688	30.775766478017307	25.940006624328564
11	39.505479471781804	19.18463155772526	19.047824772108697	22.26206419838424
12	24.347287625466222	22.947538197894616	26.046571909966737	26.658602266672425
13	33.46293976181219	17.112368773491166	21.409541913278897	28.01514955141775
14	25.21277055341945	18.38251177258392	21.818522198701057	34.586195475295575
15	28.435650408260248	24.64970262525021	19.2868766290808	27.627770337408737
16	28.059791765671577	23.20675105485232	21.290015984792845	27.443441194683256
17	26.749326766607624	23.454443340389684	22.495355769646174	27.300874123356518
18	27.025820480695845	22.04605348425282	23.104505983496782	27.82362005155456
19	27.64505119453925	22.591840555291544	22.93457755504673	26.828530695122478
20	28.051151337106322	22.135337912760473	22.600480983856798	27.21302976627641
21	28.69774340807304	22.387350412580464	21.96540948431042	26.949496695036075
22	29.183047479154965	21.926527555766768	21.592430984576836	27.297993980501435
23	27.603289123140506	22.227502484123214	22.321107126913496	27.848101265822784
24	27.63641076597399	22.440633055399548	21.80556155585317	28.11739462277329
25	27.760256908742676	22.074854912803676	22.038853127115104	28.126035051338544
26	27.740095908757073	22.417591912558862	22.40031105542835	27.442001123255714
27	27.606169265995597	22.57455969816103	21.79548105586037	28.023789979983004
28	27.166947480595038	22.27214469837704	22.761768983741593	27.79913883728633
29	26.6542820523898	22.39599084114572	23.53652741175962	27.413199694704858
30	26.945176480753446	22.884175055082732	22.766089198024222	27.4045592661396
31	27.525525266053197	22.387350412580464	22.391670626863093	27.695453694503247
32	26.54627669532409	22.342708198326637	23.000820840713697	28.110194265635574
33	26.1632176955977	22.311026626920697	23.37667948330237	28.14907619417923
34	27.39303869471926	21.81564205584597	23.215391483417577	27.575927766017195
35	26.609639838135973	22.321107126913496	23.830301982978355	27.238951051972176
36	26.255382266960442	21.802681412998083	23.660373554528306	28.28156276551317
37	25.7124753387768	20.853674342247373	25.05436269638974	28.37948762258608
38	25.260292910528364	20.86663498509526	26.052332195676907	27.820739908699473
39	24.993879696432945	20.381330914013336	26.822770409412307	27.802018980141412
40	24.551777768177303	20.41445255684682	27.554326694604054	27.479442980371825
41	24.003110554283495	20.012672628562377	28.36796705116574	27.616249765988393
42	23.27731455480192	20.26180498552728	28.709263979493382	27.751616480177415
43	22.319667055485954	20.31652769977391	28.972797050733718	28.391008194006425
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	3.5
7	5.0
8	3.5
9	2.0
10	4.5
11	7.0
12	7.0
13	13.0
14	19.0
15	26.0
16	33.0
17	36.5
18	40.0
19	40.0
20	53.5
21	67.0
22	88.0
23	109.0
24	132.0
25	155.0
26	155.0
27	214.5
28	274.0
29	359.0
30	444.0
31	602.5
32	761.0
33	761.0
34	1023.0
35	1285.0
36	1509.5
37	1734.0
38	2028.0
39	2322.0
40	2322.0
41	2674.5
42	3027.0
43	3429.5
44	3832.0
45	4127.0
46	4422.0
47	4422.0
48	4624.0
49	4826.0
50	5194.5
51	5563.0
52	5677.5
53	5792.0
54	5792.0
55	5912.0
56	6032.0
57	5801.5
58	5571.0
59	5403.0
60	5235.0
61	5235.0
62	4895.5
63	4556.0
64	4143.0
65	3730.0
66	3279.5
67	2829.0
68	2829.0
69	2520.0
70	2211.0
71	1925.0
72	1639.0
73	1375.5
74	1112.0
75	1112.0
76	922.5
77	733.0
78	588.0
79	443.0
80	368.0
81	293.0
82	293.0
83	229.5
84	166.0
85	131.0
86	96.0
87	69.5
88	43.0
89	43.0
90	31.5
91	20.0
92	13.0
93	6.0
94	4.5
95	3.0
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	69441.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.48899065393644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.8765979205727	77.62561023026741
2	4.7128004090676665	7.963594994311718
3	1.467530253962843	3.719704497343068
4	0.6664394068518834	2.252271712676949
5	0.42270325549684673	1.7856885701530794
6	0.24544059996591105	1.2442217133969844
7	0.1806715527526845	1.068532999236762
8	0.115902505539458	0.7833988565832865
9	0.08351798193284472	0.6350714995463775
>10	0.22498721663541843	2.695813712360133
>50	0.0017044486108743822	0.07632378565976873
>100	0.0017044486108743822	0.14976742846445182
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	104	0.14976742846445182	RNA PCR Primer, Index 17 (95% over 21bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.001440071427542806	0.0
18	0.0	0.0	0.0	0.001440071427542806	0.0
19	0.0	0.0	0.0	0.001440071427542806	0.0
20	0.0	0.0	0.0	0.001440071427542806	0.0
21	0.0	0.0	0.0	0.001440071427542806	0.0
22	0.0	0.0	0.0	0.001440071427542806	0.0
23	0.0	0.0	0.0	0.001440071427542806	0.0
24	0.0	0.0	0.0	0.002880142855085612	0.0
25	0.0	0.0	0.0	0.005760285710171224	0.0
26	0.0	0.0	0.0	0.008640428565256836	0.0
27	0.0	0.0	0.0	0.043202142826284186	0.0
28	0.0	0.001440071427542806	0.0	0.14400714275428061	0.0
29	0.0	0.001440071427542806	0.0	0.2520124998199911	0.0
30	0.0	0.001440071427542806	0.0	0.4003398568569001	0.0
31	0.0	0.001440071427542806	0.0	0.8568424993879696	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	65	2.8885552E-9	28.461538	37
GTATCAA	45	0.0037902396	20.555557	1
ATCAACG	50	0.0069701066	18.499998	3
TCTTATA	130	1.3285899E-8	17.076923	37
CTCTTAT	225	0.0	16.444445	37
GTCTCTT	475	0.0	12.463158	37
TCTCTTA	305	1.8189894E-12	12.131147	36
GAGTACA	115	0.0051349984	11.26087	6
AGAGTAC	115	0.0051349984	11.26087	5
CAGAGTA	115	0.0051349984	11.26087	4
AGTACAT	115	0.0051349984	11.26087	7
TGTCTCT	645	0.0	9.178295	36
CTGTCTC	805	0.0	7.583851	37
CCTGTCT	435	1.2336586E-6	7.229885	37
GCTGTCT	325	7.5472053E-4	6.830769	35
>>END_MODULE