FastQCFastQC Report
Fri 10 Feb 2017
ERR1632155.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632155.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560713
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA5810.10361807199048355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3000.022.1999991
TTAGACC500.007030498218.54
TAAGTCA609.2287734E-418.54
CGTCAGA500.007030498218.51
GCCGTCT701.2177842E-418.536
ATACTGA752.0648887E-417.2666666
ATGCCGT752.0648887E-417.26666634
TTAGAGT650.0015785717.0769234
TCTTATA5050.016.85148637
TCTAGCG904.439424E-516.44444528
TCGCACG803.378702E-416.187522
CCGATCG803.378702E-416.187518
TCGTATG803.378702E-416.187530
TATATCA1059.325957E-615.8571442
GACGGAC1059.325957E-615.8571447
CGTATGC855.3580676E-415.23529431
TAGGCAG855.3580676E-415.2352945
GTCTTGC1701.4788384E-915.2352941
CTCTTAT8650.015.18497137
GCGAAAG1101.44915375E-515.13636418
Produced by FastQC (version 0.11.5)