##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632155.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	560713
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.80409228963837	34.0	31.0	34.0	31.0	34.0
2	32.97728249567961	34.0	31.0	34.0	31.0	34.0
3	33.050738969847316	34.0	33.0	34.0	31.0	34.0
4	36.43190009862443	37.0	37.0	37.0	35.0	37.0
5	36.30596936400619	37.0	37.0	37.0	35.0	37.0
6	36.33232866011667	37.0	37.0	37.0	35.0	37.0
7	36.310376253092045	37.0	37.0	37.0	35.0	37.0
8	36.295315072060035	37.0	37.0	37.0	35.0	37.0
9	38.09349167934398	39.0	39.0	39.0	37.0	39.0
10	38.07016958765001	39.0	38.0	39.0	35.0	39.0
11	38.12487315257538	39.0	38.0	39.0	37.0	39.0
12	38.10275488529783	39.0	38.0	39.0	37.0	39.0
13	38.114650453975564	39.0	38.0	39.0	37.0	39.0
14	39.52441980121738	40.0	39.0	41.0	37.0	41.0
15	39.542450415809874	41.0	39.0	41.0	37.0	41.0
16	39.526561716956806	41.0	39.0	41.0	37.0	41.0
17	39.51731099510801	41.0	39.0	41.0	37.0	41.0
18	39.50053057446501	41.0	39.0	41.0	37.0	41.0
19	39.531730136451266	41.0	39.0	41.0	37.0	41.0
20	39.51093518430998	41.0	39.0	41.0	37.0	41.0
21	39.47180999905478	41.0	39.0	41.0	37.0	41.0
22	39.44793147296389	40.0	39.0	41.0	37.0	41.0
23	39.39675199255234	40.0	39.0	41.0	37.0	41.0
24	39.34368741227687	40.0	39.0	41.0	36.0	41.0
25	39.28904983476395	40.0	39.0	41.0	36.0	41.0
26	39.215539857288846	40.0	39.0	41.0	36.0	41.0
27	39.13795292779015	40.0	39.0	41.0	36.0	41.0
28	39.063788426521235	40.0	39.0	41.0	36.0	41.0
29	39.010500915798275	40.0	39.0	41.0	35.0	41.0
30	38.93956088052177	40.0	39.0	41.0	35.0	41.0
31	38.87909857627699	40.0	38.0	41.0	35.0	41.0
32	38.796899661680754	40.0	38.0	41.0	35.0	41.0
33	38.71698177142317	40.0	38.0	41.0	35.0	41.0
34	38.648831041905574	40.0	38.0	41.0	35.0	41.0
35	38.61998562544475	40.0	38.0	41.0	35.0	41.0
36	38.587988864178286	40.0	38.0	41.0	35.0	41.0
37	38.53184784372754	40.0	38.0	41.0	35.0	41.0
38	38.47414274325725	40.0	38.0	41.0	35.0	41.0
39	38.411162216677695	40.0	38.0	41.0	34.0	41.0
40	38.348417104650686	40.0	38.0	41.0	34.0	41.0
41	38.29850743606801	40.0	38.0	41.0	34.0	41.0
42	38.2453126644112	40.0	38.0	41.0	34.0	41.0
43	37.3721779234653	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	0.0
16	2.0
17	6.0
18	14.0
19	16.0
20	19.0
21	82.0
22	122.0
23	249.0
24	362.0
25	570.0
26	771.0
27	1253.0
28	1674.0
29	2395.0
30	3210.0
31	4614.0
32	6211.0
33	8820.0
34	13056.0
35	19302.0
36	31807.0
37	57981.0
38	142580.0
39	265595.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.91803471651273	17.65359461970741	12.370321358698657	27.058049305081212
2	20.382441641267455	19.74539559453767	32.68338704470914	27.188775719485726
3	21.066570598505834	20.896251736628184	28.49586151917291	29.541316145693074
4	16.254839820014872	15.162480627344113	31.602441890949557	36.980237661691454
5	16.975529370640597	34.03862582105284	32.29156449021157	16.694280318094997
6	36.858428465186286	31.90206041236782	15.001792360797769	16.237718761648114
7	31.083459809207202	28.13703267090294	19.83207095251938	20.947436567370474
8	28.041440095021876	31.203663906490487	19.437573232651996	21.31732276583564
9	27.005259375117042	13.899980917153695	18.840476322111314	40.25428338561795
10	18.645367594473463	25.322045324435138	31.275358338401286	24.75722874269011
11	37.76174263839076	20.279536946708923	19.857217506995557	22.10150290790476
12	23.11717402664108	23.828411326293487	26.66372992957181	26.390684717493617
13	31.61760116137846	18.05005412751265	23.35455036712186	26.977794343987032
14	24.49167399364737	19.820656913608207	22.315872826205208	33.371796266539214
15	27.522458013279522	25.999753884785974	20.25171522686294	26.22607287507156
16	27.60217794129974	24.217915404137234	21.89979543902139	26.28011121554164
17	26.145104536545432	24.647011929454106	22.929199073322714	26.27868446067774
18	25.48095014740161	23.23077938267884	24.563903458632133	26.724367011287413
19	26.691373305059805	23.752436629791	23.911698141473444	25.64449192367575
20	26.868290908182974	22.983415758150784	23.991418069493662	26.15687526417258
21	27.156138701974093	23.72871683017872	23.078829989673864	26.036314478173328
22	27.368725176694674	22.94293158888772	22.850192522734446	26.838150711683163
23	26.078225402300287	23.40751864144402	23.47600287491105	27.038253081344642
24	26.811755746701078	23.82520112784972	23.188690114193893	26.174353011255313
25	26.90074958133662	23.071339526638404	23.130193164774134	26.897717727250843
26	26.789284357594706	23.91562171734916	23.22204050913747	26.07305341591866
27	27.089259567728945	22.87302060055679	23.10130137878023	26.93641845293403
28	25.880976542366597	23.329938845719646	23.975545421632813	26.813539190280945
29	25.567625505383322	23.6035190908718	24.185100042267614	26.64375536147726
30	25.41068247035471	24.48329180882198	24.007647406070483	26.098378314752825
31	26.695653569651494	23.634907697877523	23.353658645331926	26.31578008713905
32	25.661256293326534	23.187976736761946	23.878347746529865	27.27241922338166
33	25.57190576997501	23.10825680874173	23.98606773875405	27.33376968252921
34	26.62342410466674	22.91992516670739	23.842500530574466	26.614150198051412
35	26.261028369237028	22.968078143363897	24.41623433021885	26.354659157180233
36	25.39480982249386	23.38558228541161	24.55213273100499	26.667475161089545
37	26.40085034589888	21.86947689816359	24.828566485884938	26.901106270052594
38	25.021713425584924	22.10185959662073	26.190047314758175	26.68637966303617
39	25.144057655164048	21.67793505768548	26.42528352294311	26.75272376420736
40	24.536438427502127	21.699158036285944	27.19947638096495	26.56492715524698
41	23.70446199749248	22.01892947015675	27.997032349883096	26.27957618246768
42	24.286756326320237	21.707540221111334	27.496776425729387	26.50892702683904
43	23.08756886321523	21.461603351447174	28.181975449115683	27.268852336221915
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	7.5
2	7.0
3	25.0
4	43.0
5	43.0
6	64.5
7	86.0
8	85.0
9	84.0
10	127.5
11	171.0
12	171.0
13	288.0
14	405.0
15	603.0
16	801.0
17	806.5
18	812.0
19	812.0
20	1012.5
21	1213.0
22	1366.0
23	1519.0
24	1982.0
25	2445.0
26	2445.0
27	2916.0
28	3387.0
29	5017.0
30	6647.0
31	7945.0
32	9243.0
33	9243.0
34	10944.0
35	12645.0
36	14634.0
37	16623.0
38	20154.0
39	23685.0
40	23685.0
41	26109.5
42	28534.0
43	29185.5
44	29837.0
45	32915.0
46	35993.0
47	35993.0
48	38664.0
49	41335.0
50	44503.5
51	47672.0
52	50604.0
53	53536.0
54	53536.0
55	49170.0
56	44804.0
57	43235.0
58	41666.0
59	38837.5
60	36009.0
61	36009.0
62	34066.0
63	32123.0
64	29231.0
65	26339.0
66	23414.0
67	20489.0
68	20489.0
69	17666.5
70	14844.0
71	12873.0
72	10902.0
73	8805.5
74	6709.0
75	6709.0
76	5305.0
77	3901.0
78	3273.0
79	2645.0
80	2149.5
81	1654.0
82	1654.0
83	1255.0
84	856.0
85	694.0
86	532.0
87	416.5
88	301.0
89	301.0
90	232.0
91	163.0
92	97.5
93	32.0
94	20.0
95	8.0
96	8.0
97	6.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	560713.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.38145878869342
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.44373982858167	53.40362031577427
2	6.378483191326708	7.702842399029347
3	1.7479421756330857	3.166298953290249
4	0.8540348978325171	2.062714919503205
5	0.4629529546245812	1.3976887375384002
6	0.31645935815156867	1.1464966615515189
7	0.22546303839077747	0.9529651012676433
8	0.18260135577241313	0.8820588990665207
9	0.12518550327936878	0.6802994976484547
>10	1.0160866843233978	12.796963744811066
>50	0.15917777961808902	6.744406356772853
>100	0.08757736299621366	8.959848588375191
>500	2.958694695818007E-4	0.10379582537128314
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	581	0.10361807199048355	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.783443579870629E-4	0.0
10	0.0	0.0	0.0	1.783443579870629E-4	0.0
11	0.0	0.0	0.0	1.783443579870629E-4	0.0
12	0.0	0.0	0.0	1.783443579870629E-4	0.0
13	0.0	0.0	0.0	3.566887159741258E-4	0.0
14	0.0	0.0	0.0	7.133774319482516E-4	0.0
15	0.0	1.783443579870629E-4	0.0	7.133774319482516E-4	0.0
16	0.0	1.783443579870629E-4	0.0	8.917217899353145E-4	0.0
17	0.0	1.783443579870629E-4	0.0	0.0014267548638965033	0.0
18	0.0	1.783443579870629E-4	0.0	0.0014267548638965033	0.0
19	0.0	1.783443579870629E-4	0.0	0.001605099221883566	0.0
20	0.0	1.783443579870629E-4	0.0	0.001783443579870629	0.0
21	0.0	1.783443579870629E-4	0.0	0.001783443579870629	0.0
22	0.0	1.783443579870629E-4	0.0	0.0024968210118188807	0.0
23	0.0	1.783443579870629E-4	0.0	0.003566887159741258	0.0
24	0.0	1.783443579870629E-4	0.0	0.00552867509759895	0.0
25	0.0	1.783443579870629E-4	0.0	0.00677708560350839	0.0
26	0.0	1.783443579870629E-4	0.0	0.012662449417081466	0.0
27	0.0	1.783443579870629E-4	0.0	0.030675229573774818	0.0
28	0.0	1.783443579870629E-4	0.0	0.08863714591957027	0.0
29	0.0	1.783443579870629E-4	0.0	0.1630067432001755	0.0
30	0.0	1.783443579870629E-4	0.0	0.26698150390663317	0.0
31	0.0	1.783443579870629E-4	0.0	0.5525108210439209	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	300	0.0	22.199999	1
TTAGACC	50	0.0070304982	18.5	4
TAAGTCA	60	9.2287734E-4	18.5	4
CGTCAGA	50	0.0070304982	18.5	1
GCCGTCT	70	1.2177842E-4	18.5	36
ATACTGA	75	2.0648887E-4	17.266666	6
ATGCCGT	75	2.0648887E-4	17.266666	34
TTAGAGT	65	0.00157857	17.076923	4
TCTTATA	505	0.0	16.851486	37
TCTAGCG	90	4.439424E-5	16.444445	28
TCGCACG	80	3.378702E-4	16.1875	22
CCGATCG	80	3.378702E-4	16.1875	18
TCGTATG	80	3.378702E-4	16.1875	30
TATATCA	105	9.325957E-6	15.857144	2
GACGGAC	105	9.325957E-6	15.857144	7
CGTATGC	85	5.3580676E-4	15.235294	31
TAGGCAG	85	5.3580676E-4	15.235294	5
GTCTTGC	170	1.4788384E-9	15.235294	1
CTCTTAT	865	0.0	15.184971	37
GCGAAAG	110	1.44915375E-5	15.136364	18
>>END_MODULE