FastQCFastQC Report
Fri 10 Feb 2017
ERR1632154.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632154.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2341025
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57880.24724212684614646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT45650.1950000533954144No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40550.17321472431947543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19800.021.7702031
CTTATAC20550.016.56447837
TATACAC6900.016.08695637
TCTATAC1504.6784407E-916.0333353
GTATCAA28400.015.1778172
TATAGGA1252.961735E-614.7999992
CGAACGA3650.014.6986316
TAATACT2550.014.5098044
TCTTATA32300.014.37616237
ACGGACC5800.014.0344838
TAACGCC4350.014.0344824
ATAACGA3700.014.012
TAATACG800.00630119513.8754
ACGAACG3750.013.81333315
ACGTTAT950.001245748613.6315799
AGGCGTA1501.3070385E-613.5666687
CAAGACG6450.013.480624
GACGGAC5950.013.3697487
AGACGGA6150.013.2357726
TCGTTTA4250.013.05882430
Produced by FastQC (version 0.11.5)