##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632154.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2341025
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.010559049988785	33.0	31.0	34.0	30.0	34.0
2	32.20163774415053	33.0	31.0	34.0	30.0	34.0
3	32.293768327976	34.0	31.0	34.0	30.0	34.0
4	35.9425170598349	37.0	35.0	37.0	35.0	37.0
5	35.53850300616183	37.0	35.0	37.0	33.0	37.0
6	35.51954208092609	37.0	35.0	37.0	33.0	37.0
7	35.489951196591235	37.0	35.0	37.0	33.0	37.0
8	35.46569173759357	37.0	35.0	37.0	33.0	37.0
9	37.15096122425006	39.0	37.0	39.0	34.0	39.0
10	36.98718296472699	39.0	37.0	39.0	33.0	39.0
11	37.08881195202956	39.0	37.0	39.0	33.0	39.0
12	37.02387501201397	39.0	37.0	39.0	33.0	39.0
13	37.09078245640264	39.0	37.0	39.0	33.0	39.0
14	38.24996529298064	40.0	38.0	41.0	34.0	41.0
15	38.21158595059856	40.0	38.0	41.0	34.0	41.0
16	38.19529565040954	40.0	38.0	41.0	34.0	41.0
17	38.16892344165483	40.0	38.0	41.0	33.0	41.0
18	38.15951687829049	40.0	38.0	41.0	33.0	41.0
19	38.18179002787241	40.0	38.0	41.0	34.0	41.0
20	38.146772460781065	40.0	38.0	41.0	34.0	41.0
21	38.105625954443035	40.0	38.0	41.0	34.0	41.0
22	38.05696308241048	40.0	37.0	41.0	34.0	41.0
23	37.981549107762625	40.0	37.0	41.0	33.0	41.0
24	37.91318247348918	40.0	37.0	41.0	33.0	41.0
25	37.83519398554052	39.0	37.0	41.0	33.0	41.0
26	37.69864653303574	39.0	37.0	40.0	33.0	41.0
27	37.58836962441666	39.0	37.0	40.0	33.0	41.0
28	37.47971123759891	39.0	37.0	40.0	32.0	41.0
29	37.38205743210773	39.0	37.0	40.0	32.0	41.0
30	37.27784709689132	39.0	36.0	40.0	32.0	41.0
31	37.17879817601264	39.0	36.0	40.0	31.0	41.0
32	37.054960540788755	39.0	36.0	40.0	31.0	41.0
33	36.94418812272402	39.0	36.0	40.0	31.0	41.0
34	36.84797855640158	39.0	36.0	40.0	31.0	41.0
35	36.799836396450274	39.0	35.0	40.0	31.0	41.0
36	36.741625569996046	39.0	35.0	40.0	31.0	41.0
37	36.66527738917782	39.0	35.0	40.0	30.0	41.0
38	36.59673006482203	38.0	35.0	40.0	30.0	41.0
39	36.53012547922384	38.0	35.0	40.0	30.0	41.0
40	36.42630258113433	38.0	35.0	40.0	30.0	41.0
41	36.36136820409863	38.0	35.0	40.0	30.0	41.0
42	36.278325946967676	38.0	35.0	40.0	30.0	41.0
43	35.30074433207676	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	4.0
12	3.0
13	3.0
14	17.0
15	14.0
16	33.0
17	72.0
18	137.0
19	290.0
20	552.0
21	992.0
22	1550.0
23	2771.0
24	4267.0
25	6452.0
26	9565.0
27	13475.0
28	19441.0
29	26774.0
30	35824.0
31	47953.0
32	63864.0
33	86464.0
34	121995.0
35	177836.0
36	278004.0
37	446415.0
38	647736.0
39	348516.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.10889460813105	18.321675334522272	12.025117202934613	26.544312854412063
2	19.956301192853555	20.411144690893945	32.549930052007134	27.082624064245366
3	20.456167704317554	21.717068378167685	29.19417776401363	28.632586153501137
4	15.399066648156257	15.484157580546984	33.42941660170224	35.687359169594515
5	16.350316634807402	34.45969180166807	32.818615777277046	16.371375786247476
6	35.71320255016499	33.90109033436208	15.243707350412746	15.141999765060177
7	30.537392808705587	29.299729819203122	20.441772300594824	19.72110507149646
8	28.60520498499589	31.426362383998462	19.410770922993134	20.557661708012514
9	27.382492711525934	13.502546961266967	18.941745602887625	40.17321472431948
10	17.883363056780684	25.3415063914311	31.7416943432898	25.033436208498415
11	38.120438696724726	20.40977776828526	20.46052477013274	21.009258764857275
12	23.232344806228042	24.22438888948217	27.714740338099762	24.828525966190025
13	31.793808267745966	18.216165995664294	23.359853055819567	26.630172680770176
14	23.811364680001283	19.486378829786098	23.784410674811244	32.91784581540138
15	26.676477184139426	26.248374109631467	20.8916179878472	26.183530718381903
16	26.464561463461518	24.605034119669803	22.844480516013284	26.085923900855395
17	25.34201898740936	24.77564314776647	24.12738010059696	25.75495776422721
18	25.585502077081618	23.47497356926987	25.183455964801745	25.756068388846764
19	26.01398959857327	23.98641620657618	24.87769246377121	25.121901731079333
20	26.27767751305518	23.434606635982103	24.51584241945302	25.7718734315097
21	27.0725430100063	23.76275349472987	23.98410952467402	25.180593970589804
22	26.97339840454502	23.59056396236691	23.967108424728483	25.468929208359587
23	25.923858139063015	23.380954923591162	24.342072382823762	26.353114554522055
24	25.82719108082998	24.05254108777138	24.09649619290695	26.023771638491688
25	25.987676338356064	23.288089618863534	24.346813895622642	26.37742014715776
26	25.786012537243302	24.082357087173353	24.415587189372175	25.716043186211166
27	26.00685597120919	23.61879945750259	24.23109535353104	26.14324921775718
28	25.525272049636378	23.776123706496087	24.887688085347232	25.810916158520307
29	25.276748432844588	23.99491675654895	24.896573082303693	25.831761728302773
30	25.180978417573495	24.395126066573404	24.728356168772226	25.69553934708087
31	25.871915079932933	24.166892707254302	24.078811631657075	25.88238058115569
32	25.080125158851356	23.93370425347871	24.443310088529596	26.542860499140335
33	24.78978225350007	23.539517946198778	25.248982817355646	26.421716982945505
34	25.64406616759753	23.26382674255935	25.129334372764067	25.96277271707906
35	25.603058489336938	23.42008308326481	25.495584199228972	25.481274228169287
36	24.884527076814646	23.701412842665075	25.21549321344283	26.198566867077457
37	24.97551286295533	22.828632757018827	25.862731068655826	26.33312331137002
38	24.35518629660085	22.613470595145287	26.848154120524132	26.183188987729732
39	24.40888072532331	22.176696105338472	27.00265909163721	26.411764077701005
40	24.030883907690008	22.391601969222883	27.75391121410493	25.823602908982174
41	23.297401779135207	22.30129964438654	28.372785425187686	26.028513151290568
42	23.413504768210505	22.429405922619367	28.42776134385579	25.72932796531434
43	22.591599833406306	21.776059631998805	28.809218184342328	26.82312235025256
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	144.0
1	153.0
2	162.0
3	453.5
4	745.0
5	745.0
6	985.5
7	1226.0
8	1209.0
9	1192.0
10	1770.5
11	2349.0
12	2349.0
13	4000.0
14	5651.0
15	8695.0
16	11739.0
17	11041.5
18	10344.0
19	10344.0
20	11662.5
21	12981.0
22	12316.0
23	11651.0
24	13774.0
25	15897.0
26	15897.0
27	19250.0
28	22603.0
29	27739.0
30	32875.0
31	39451.0
32	46027.0
33	46027.0
34	55147.5
35	64268.0
36	73982.0
37	83696.0
38	92910.5
39	102125.0
40	102125.0
41	111592.5
42	121060.0
43	128291.5
44	135523.0
45	143309.5
46	151096.0
47	151096.0
48	158280.5
49	165465.0
50	169759.5
51	174054.0
52	176662.5
53	179271.0
54	179271.0
55	177681.5
56	176092.0
57	171622.5
58	167153.0
59	161481.0
60	155809.0
61	155809.0
62	143094.0
63	130379.0
64	117621.5
65	104864.0
66	92691.5
67	80519.0
68	80519.0
69	69821.0
70	59123.0
71	51119.0
72	43115.0
73	34623.0
74	26131.0
75	26131.0
76	21206.5
77	16282.0
78	13619.0
79	10956.0
80	9331.0
81	7706.0
82	7706.0
83	6114.0
84	4522.0
85	3775.5
86	3029.0
87	2480.5
88	1932.0
89	1932.0
90	1481.0
91	1030.0
92	597.5
93	165.0
94	108.0
95	51.0
96	51.0
97	33.5
98	16.0
99	11.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2341025.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.89860251618584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.63253588506097	54.49065439603656
2	7.765361050472578	9.76860716216692
3	2.1623760698467263	4.0803129872340405
4	0.974166694577799	2.450948948270224
5	0.5724092926693536	1.800187228809038
6	0.3909367297176845	1.475362438289413
7	0.25218666411786056	1.1103532122362525
8	0.18275544192850698	0.9196049519624837
9	0.13908745356102464	0.787356581087101
>10	0.7945566236167523	9.41040802611282
>50	0.07356057998128702	3.22071982756945
>100	0.05342080139283802	6.818038256047915
>500	0.005290241004287696	2.1780966349985404
>1k	0.0012886484497620003	1.2424327006616607
>5k	6.782360261905264E-5	0.24691664851770678
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5788	0.24724212684614646	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4565	0.1950000533954144	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4055	0.17321472431947543	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.271633152144894E-5
2	0.0	0.0	0.0	0.0	4.271633152144894E-5
3	0.0	0.0	0.0	0.0	4.271633152144894E-5
4	0.0	0.0	0.0	0.0	4.271633152144894E-5
5	0.0	0.0	0.0	0.0	4.271633152144894E-5
6	0.0	0.0	0.0	0.0	4.271633152144894E-5
7	0.0	0.0	0.0	0.0	4.271633152144894E-5
8	0.0	0.0	0.0	0.0	4.271633152144894E-5
9	0.0	0.0	0.0	8.543266304289788E-5	4.271633152144894E-5
10	0.0	0.0	0.0	1.7086532608579576E-4	4.271633152144894E-5
11	0.0	0.0	0.0	2.135816576072447E-4	4.271633152144894E-5
12	0.0	0.0	0.0	2.135816576072447E-4	4.271633152144894E-5
13	0.0	0.0	0.0	2.135816576072447E-4	4.271633152144894E-5
14	0.0	0.0	0.0	2.9901432065014257E-4	4.271633152144894E-5
15	0.0	0.0	0.0	4.271633152144894E-4	4.271633152144894E-5
16	0.0	0.0	0.0	8.543266304289787E-4	4.271633152144894E-5
17	0.0	0.0	0.0	0.0014523552717292639	4.271633152144894E-5
18	0.0	0.0	0.0	0.0014950716032507128	4.271633152144894E-5
19	0.0	0.0	0.0	0.0017513695923794064	4.271633152144894E-5
20	0.0	0.0	0.0	0.001964951249986651	4.271633152144894E-5
21	0.0	0.0	0.0	0.0024775472282440383	4.271633152144894E-5
22	0.0	0.0	0.0	0.0036308881793231597	4.271633152144894E-5
23	0.0	0.0	0.0	0.005296825108659669	4.271633152144894E-5
24	0.0	0.0	0.0	0.007347209021689218	4.271633152144894E-5
25	0.0	0.0	0.0	0.009397592934718766	4.271633152144894E-5
26	0.0	0.0	0.0	0.01362650975534221	4.271633152144894E-5
27	0.0	0.0	0.0	0.036009867472581454	4.271633152144894E-5
28	0.0	0.0	0.0	0.1104217169829455	4.271633152144894E-5
29	0.0	0.0	0.0	0.22438888948217128	4.271633152144894E-5
30	0.0	0.0	0.0	0.3866255166006343	4.271633152144894E-5
31	0.0	0.0	0.0	0.7664164201578368	4.271633152144894E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1980	0.0	21.770203	1
CTTATAC	2055	0.0	16.564478	37
TATACAC	690	0.0	16.086956	37
TCTATAC	150	4.6784407E-9	16.033335	3
GTATCAA	2840	0.0	15.177817	2
TATAGGA	125	2.961735E-6	14.799999	2
CGAACGA	365	0.0	14.69863	16
TAATACT	255	0.0	14.509804	4
TCTTATA	3230	0.0	14.376162	37
ACGGACC	580	0.0	14.034483	8
TAACGCC	435	0.0	14.034482	4
ATAACGA	370	0.0	14.0	12
TAATACG	80	0.006301195	13.875	4
ACGAACG	375	0.0	13.813333	15
ACGTTAT	95	0.0012457486	13.631579	9
AGGCGTA	150	1.3070385E-6	13.566668	7
CAAGACG	645	0.0	13.48062	4
GACGGAC	595	0.0	13.369748	7
AGACGGA	615	0.0	13.235772	6
TCGTTTA	425	0.0	13.058824	30
>>END_MODULE