FastQCFastQC Report
Fri 10 Feb 2017
ERR1632153.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632153.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578885
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA7560.13059588692054552No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC6560.11332129870354214No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT5910.10209281636248996No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTT200.001841181137.018
GGTATCA1900.028.2368431
GTACTAT358.864057E-426.428571
GTATATA358.864057E-426.428571
GTATAGA601.3354911E-624.6666681
GTGTTAG502.699858E-422.1999991
TGTACCG502.699858E-422.1999995
CCTAAGC806.94783E-720.81253
CGATTTG450.003823548320.55555528
GCATTAA450.003823548320.5555551
CTTATAT450.003823548320.5555551
GCGATTT450.003823548320.55555527
TAGGGTT555.138855E-420.1818185
TATAGAA759.2527425E-619.7333352
TCCTAAG851.2431228E-619.5882362
GCACCGT701.2178713E-418.56
ATACCCA609.2292664E-418.54
TATATAT500.00703076818.4999982
TATACAG500.00703076818.4999985
TAAGCCC500.00703076818.4999984
Produced by FastQC (version 0.11.5)